Transcriptional activation of the adenoviral genome is mediated by capsid protein VI.

Gene expression of DNA viruses requires nuclear import of the viral genome. Human Adenoviruses (Ads), like most DNA viruses, encode factors within early transcription units promoting their own gene expression and counteracting cellular antiviral defense mechanisms. The cellular transcriptional repre...

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Autores principales:	Sabrina Schreiner, Ruben Martinez, Peter Groitl, Fabienne Rayne, Remi Vaillant, Peter Wimmer, Guillaume Bossis, Thomas Sternsdorf, Lisa Marcinowski, Zsolt Ruzsics, Thomas Dobner, Harald Wodrich
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9a7b10c4f48d44289d02c77faa09275f
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spelling	oai:doaj.org-article:9a7b10c4f48d44289d02c77faa09275f2021-11-18T06:04:43ZTranscriptional activation of the adenoviral genome is mediated by capsid protein VI.1553-73661553-737410.1371/journal.ppat.1002549https://doaj.org/article/9a7b10c4f48d44289d02c77faa09275f2012-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22427750/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Gene expression of DNA viruses requires nuclear import of the viral genome. Human Adenoviruses (Ads), like most DNA viruses, encode factors within early transcription units promoting their own gene expression and counteracting cellular antiviral defense mechanisms. The cellular transcriptional repressor Daxx prevents viral gene expression through the assembly of repressive chromatin remodeling complexes targeting incoming viral genomes. However, it has remained unclear how initial transcriptional activation of the adenoviral genome is achieved. Here we show that Daxx mediated repression of the immediate early Ad E1A promoter is efficiently counteracted by the capsid protein VI. This requires a conserved PPxY motif in protein VI. Capsid proteins from other DNA viruses were also shown to activate the Ad E1A promoter independent of Ad gene expression and support virus replication. Our results show how Ad entry is connected to transcriptional activation of their genome in the nucleus. Our data further suggest a common principle for genome activation of DNA viruses by counteracting Daxx related repressive mechanisms through virion proteins.Sabrina SchreinerRuben MartinezPeter GroitlFabienne RayneRemi VaillantPeter WimmerGuillaume BossisThomas SternsdorfLisa MarcinowskiZsolt RuzsicsThomas DobnerHarald WodrichPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 2, p e1002549 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Sabrina Schreiner Ruben Martinez Peter Groitl Fabienne Rayne Remi Vaillant Peter Wimmer Guillaume Bossis Thomas Sternsdorf Lisa Marcinowski Zsolt Ruzsics Thomas Dobner Harald Wodrich Transcriptional activation of the adenoviral genome is mediated by capsid protein VI.
description	Gene expression of DNA viruses requires nuclear import of the viral genome. Human Adenoviruses (Ads), like most DNA viruses, encode factors within early transcription units promoting their own gene expression and counteracting cellular antiviral defense mechanisms. The cellular transcriptional repressor Daxx prevents viral gene expression through the assembly of repressive chromatin remodeling complexes targeting incoming viral genomes. However, it has remained unclear how initial transcriptional activation of the adenoviral genome is achieved. Here we show that Daxx mediated repression of the immediate early Ad E1A promoter is efficiently counteracted by the capsid protein VI. This requires a conserved PPxY motif in protein VI. Capsid proteins from other DNA viruses were also shown to activate the Ad E1A promoter independent of Ad gene expression and support virus replication. Our results show how Ad entry is connected to transcriptional activation of their genome in the nucleus. Our data further suggest a common principle for genome activation of DNA viruses by counteracting Daxx related repressive mechanisms through virion proteins.
format	article
author	Sabrina Schreiner Ruben Martinez Peter Groitl Fabienne Rayne Remi Vaillant Peter Wimmer Guillaume Bossis Thomas Sternsdorf Lisa Marcinowski Zsolt Ruzsics Thomas Dobner Harald Wodrich
author_facet	Sabrina Schreiner Ruben Martinez Peter Groitl Fabienne Rayne Remi Vaillant Peter Wimmer Guillaume Bossis Thomas Sternsdorf Lisa Marcinowski Zsolt Ruzsics Thomas Dobner Harald Wodrich
author_sort	Sabrina Schreiner
title	Transcriptional activation of the adenoviral genome is mediated by capsid protein VI.
title_short	Transcriptional activation of the adenoviral genome is mediated by capsid protein VI.
title_full	Transcriptional activation of the adenoviral genome is mediated by capsid protein VI.
title_fullStr	Transcriptional activation of the adenoviral genome is mediated by capsid protein VI.
title_full_unstemmed	Transcriptional activation of the adenoviral genome is mediated by capsid protein VI.
title_sort	transcriptional activation of the adenoviral genome is mediated by capsid protein vi.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9a7b10c4f48d44289d02c77faa09275f
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Transcriptional activation of the adenoviral genome is mediated by capsid protein VI.

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