Clinical and analytical comparison of six Simoa assays for plasma P-tau isoforms P-tau181, P-tau217, and P-tau231

Abstract Introduction Studies using different assays and technologies showed highly promising diagnostic value of plasma phosphorylated (P-)tau levels for Alzheimer’s disease (AD). We aimed to compare six P-tau Simoa assays, including three P-tau181 (Eli Lilly, ADx, Quanterix), one P-tau217 (Eli Lil...

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Autores principales:	Sherif Bayoumy, Inge M. W. Verberk, Ben den Dulk, Zulaiga Hussainali, Marissa Zwan, Wiesje M. van der Flier, Nicholas J. Ashton, Henrik Zetterberg, Kaj Blennow, Jeroen Vanbrabant, Erik Stoops, Eugeen Vanmechelen, Jeffrey L. Dage, Charlotte E. Teunissen
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Alzheimer’s disease Blood biomarkers P-tau181 P-tau217 P-tau231 Phosphorylated tau proteins Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/9a80f98ef3db432c84a8599a0bf54390
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spelling	oai:doaj.org-article:9a80f98ef3db432c84a8599a0bf543902021-12-05T12:24:30ZClinical and analytical comparison of six Simoa assays for plasma P-tau isoforms P-tau181, P-tau217, and P-tau23110.1186/s13195-021-00939-91758-9193https://doaj.org/article/9a80f98ef3db432c84a8599a0bf543902021-12-01T00:00:00Zhttps://doi.org/10.1186/s13195-021-00939-9https://doaj.org/toc/1758-9193Abstract Introduction Studies using different assays and technologies showed highly promising diagnostic value of plasma phosphorylated (P-)tau levels for Alzheimer’s disease (AD). We aimed to compare six P-tau Simoa assays, including three P-tau181 (Eli Lilly, ADx, Quanterix), one P-tau217 (Eli Lilly), and two P-tau231 (ADx, Gothenburg). Methods We studied the analytical (sensitivity, precision, parallelism, dilution linearity, and recovery) and clinical (40 AD dementia patients, age 66±8years, 50%F; 40 age- and sex-matched controls) performance of the assays. Results All assays showed robust analytical performance, and particularly P-tau217 Eli Lilly; P-tau231 Gothenburg and all P-tau181 assays showed robust clinical performance to differentiate AD from controls, with AUCs 0.936–0.995 (P-tau231 ADx: AUC = 0.719). Results obtained with all P-tau181 assays, P-tau217 Eli Lilly assay, and P-tau231 Gothenburg assay strongly correlated (Spearman’s rho > 0.86), while correlations with P-tau231 ADx results were moderate (rho < 0.65). Discussion P-tau isoforms can be measured robustly by several novel high-sensitive Simoa assays.Sherif BayoumyInge M. W. VerberkBen den DulkZulaiga HussainaliMarissa ZwanWiesje M. van der FlierNicholas J. AshtonHenrik ZetterbergKaj BlennowJeroen VanbrabantErik StoopsEugeen VanmechelenJeffrey L. DageCharlotte E. TeunissenBMCarticleAlzheimer’s diseaseBlood biomarkersP-tau181P-tau217P-tau231Phosphorylated tau proteinsNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENAlzheimer’s Research & Therapy, Vol 13, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Alzheimer’s disease Blood biomarkers P-tau181 P-tau217 P-tau231 Phosphorylated tau proteins Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Alzheimer’s disease Blood biomarkers P-tau181 P-tau217 P-tau231 Phosphorylated tau proteins Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Sherif Bayoumy Inge M. W. Verberk Ben den Dulk Zulaiga Hussainali Marissa Zwan Wiesje M. van der Flier Nicholas J. Ashton Henrik Zetterberg Kaj Blennow Jeroen Vanbrabant Erik Stoops Eugeen Vanmechelen Jeffrey L. Dage Charlotte E. Teunissen Clinical and analytical comparison of six Simoa assays for plasma P-tau isoforms P-tau181, P-tau217, and P-tau231
description	Abstract Introduction Studies using different assays and technologies showed highly promising diagnostic value of plasma phosphorylated (P-)tau levels for Alzheimer’s disease (AD). We aimed to compare six P-tau Simoa assays, including three P-tau181 (Eli Lilly, ADx, Quanterix), one P-tau217 (Eli Lilly), and two P-tau231 (ADx, Gothenburg). Methods We studied the analytical (sensitivity, precision, parallelism, dilution linearity, and recovery) and clinical (40 AD dementia patients, age 66±8years, 50%F; 40 age- and sex-matched controls) performance of the assays. Results All assays showed robust analytical performance, and particularly P-tau217 Eli Lilly; P-tau231 Gothenburg and all P-tau181 assays showed robust clinical performance to differentiate AD from controls, with AUCs 0.936–0.995 (P-tau231 ADx: AUC = 0.719). Results obtained with all P-tau181 assays, P-tau217 Eli Lilly assay, and P-tau231 Gothenburg assay strongly correlated (Spearman’s rho > 0.86), while correlations with P-tau231 ADx results were moderate (rho < 0.65). Discussion P-tau isoforms can be measured robustly by several novel high-sensitive Simoa assays.
format	article
author	Sherif Bayoumy Inge M. W. Verberk Ben den Dulk Zulaiga Hussainali Marissa Zwan Wiesje M. van der Flier Nicholas J. Ashton Henrik Zetterberg Kaj Blennow Jeroen Vanbrabant Erik Stoops Eugeen Vanmechelen Jeffrey L. Dage Charlotte E. Teunissen
author_facet	Sherif Bayoumy Inge M. W. Verberk Ben den Dulk Zulaiga Hussainali Marissa Zwan Wiesje M. van der Flier Nicholas J. Ashton Henrik Zetterberg Kaj Blennow Jeroen Vanbrabant Erik Stoops Eugeen Vanmechelen Jeffrey L. Dage Charlotte E. Teunissen
author_sort	Sherif Bayoumy
title	Clinical and analytical comparison of six Simoa assays for plasma P-tau isoforms P-tau181, P-tau217, and P-tau231
title_short	Clinical and analytical comparison of six Simoa assays for plasma P-tau isoforms P-tau181, P-tau217, and P-tau231
title_full	Clinical and analytical comparison of six Simoa assays for plasma P-tau isoforms P-tau181, P-tau217, and P-tau231
title_fullStr	Clinical and analytical comparison of six Simoa assays for plasma P-tau isoforms P-tau181, P-tau217, and P-tau231
title_full_unstemmed	Clinical and analytical comparison of six Simoa assays for plasma P-tau isoforms P-tau181, P-tau217, and P-tau231
title_sort	clinical and analytical comparison of six simoa assays for plasma p-tau isoforms p-tau181, p-tau217, and p-tau231
publisher	BMC
publishDate	2021
url	https://doaj.org/article/9a80f98ef3db432c84a8599a0bf54390
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Clinical and analytical comparison of six Simoa assays for plasma P-tau isoforms P-tau181, P-tau217, and P-tau231

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