Dopamine 2 Receptor Activation Entrains Circadian Clocks in Mouse Retinal Pigment Epithelium

Abstract Many of the physiological, cellular, and molecular rhythms that are present within the eye are under the control of circadian clocks. Experimental evidence suggests that the retinal circadian clock, or its output signals (e.g., dopamine and melatonin), may contribute to eye disease and path...

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Autores principales:	Kenkichi Baba, Jason P. DeBruyne, Gianluca Tosini
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a86b5ab9f60454b992b71fa807d3a56
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spelling	oai:doaj.org-article:9a86b5ab9f60454b992b71fa807d3a562021-12-02T11:52:57ZDopamine 2 Receptor Activation Entrains Circadian Clocks in Mouse Retinal Pigment Epithelium10.1038/s41598-017-05394-x2045-2322https://doaj.org/article/9a86b5ab9f60454b992b71fa807d3a562017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05394-xhttps://doaj.org/toc/2045-2322Abstract Many of the physiological, cellular, and molecular rhythms that are present within the eye are under the control of circadian clocks. Experimental evidence suggests that the retinal circadian clock, or its output signals (e.g., dopamine and melatonin), may contribute to eye disease and pathology. We recently developed a retinal pigment ephithelium (RPE)-choroid preparation to monitor the circadian clock using PERIOD2 (PER2)::LUC knock-in mouse. In this study we report that dopamine, but not melatonin, is responsible for entrainment of the PER2::LUC bioluminescence rhythm in mouse RPE-choroid. Dopamine induced phase-advances of the PER2::LUC bioluminescence rhythm during the subjective day and phase-delays in the late subjective night. We found that dopamine acts exclusively through Dopamine 2 Receptors to entrain the circadian rhythm in PER2::LUC bioluminescence. Finallly, we found that DA-induced expression of core circadian clock genes Period1 and Period2 accompanied both phase advances and phase delays of the RPE-choroid clock, thus suggesting that – as in other tissues – the rapid induction of these circadian clock genes drives the resetting process. Since the RPE cells persist for the entire lifespan of an organism, we believe that RPE-choroid preparation may represent a new and unique tool to study the effects of circadian disruption during aging.Kenkichi BabaJason P. DeBruyneGianluca TosiniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kenkichi Baba Jason P. DeBruyne Gianluca Tosini Dopamine 2 Receptor Activation Entrains Circadian Clocks in Mouse Retinal Pigment Epithelium
description	Abstract Many of the physiological, cellular, and molecular rhythms that are present within the eye are under the control of circadian clocks. Experimental evidence suggests that the retinal circadian clock, or its output signals (e.g., dopamine and melatonin), may contribute to eye disease and pathology. We recently developed a retinal pigment ephithelium (RPE)-choroid preparation to monitor the circadian clock using PERIOD2 (PER2)::LUC knock-in mouse. In this study we report that dopamine, but not melatonin, is responsible for entrainment of the PER2::LUC bioluminescence rhythm in mouse RPE-choroid. Dopamine induced phase-advances of the PER2::LUC bioluminescence rhythm during the subjective day and phase-delays in the late subjective night. We found that dopamine acts exclusively through Dopamine 2 Receptors to entrain the circadian rhythm in PER2::LUC bioluminescence. Finallly, we found that DA-induced expression of core circadian clock genes Period1 and Period2 accompanied both phase advances and phase delays of the RPE-choroid clock, thus suggesting that – as in other tissues – the rapid induction of these circadian clock genes drives the resetting process. Since the RPE cells persist for the entire lifespan of an organism, we believe that RPE-choroid preparation may represent a new and unique tool to study the effects of circadian disruption during aging.
format	article
author	Kenkichi Baba Jason P. DeBruyne Gianluca Tosini
author_facet	Kenkichi Baba Jason P. DeBruyne Gianluca Tosini
author_sort	Kenkichi Baba
title	Dopamine 2 Receptor Activation Entrains Circadian Clocks in Mouse Retinal Pigment Epithelium
title_short	Dopamine 2 Receptor Activation Entrains Circadian Clocks in Mouse Retinal Pigment Epithelium
title_full	Dopamine 2 Receptor Activation Entrains Circadian Clocks in Mouse Retinal Pigment Epithelium
title_fullStr	Dopamine 2 Receptor Activation Entrains Circadian Clocks in Mouse Retinal Pigment Epithelium
title_full_unstemmed	Dopamine 2 Receptor Activation Entrains Circadian Clocks in Mouse Retinal Pigment Epithelium
title_sort	dopamine 2 receptor activation entrains circadian clocks in mouse retinal pigment epithelium
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9a86b5ab9f60454b992b71fa807d3a56
work_keys_str_mv	AT kenkichibaba dopamine2receptoractivationentrainscircadianclocksinmouseretinalpigmentepithelium AT jasonpdebruyne dopamine2receptoractivationentrainscircadianclocksinmouseretinalpigmentepithelium AT gianlucatosini dopamine2receptoractivationentrainscircadianclocksinmouseretinalpigmentepithelium
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Dopamine 2 Receptor Activation Entrains Circadian Clocks in Mouse Retinal Pigment Epithelium

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