Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner

Jia Liu,1 Yiyuan Kang,1 Suhan Yin,1 Bin Song,2 Limin Wei,3 Liangjiao Chen,4 Longquan Shao1 1Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, 2Department of Stomatology, Guizhou Provincial People’s Hospital, Guiyang, 3Department of Pediatric Dentistry, S...

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Autores principales:	Liu J, Kang Y, Yin S, Song B, Wei L, Chen L, Shao L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Zinc oxide nanoparticles SH-SY5Y cells reactive oxygen species apoptosis cell cytoskeleton Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9a88b72ab94e4911ac131115da7c85b0
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spelling	oai:doaj.org-article:9a88b72ab94e4911ac131115da7c85b02021-12-02T03:11:42ZZinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner1178-2013https://doaj.org/article/9a88b72ab94e4911ac131115da7c85b02017-11-01T00:00:00Zhttps://www.dovepress.com/zinc-oxide-nanoparticles-induce-toxic-responses-in-human-neuroblastoma-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jia Liu,1 Yiyuan Kang,1 Suhan Yin,1 Bin Song,2 Limin Wei,3 Liangjiao Chen,4 Longquan Shao1 1Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, 2Department of Stomatology, Guizhou Provincial People’s Hospital, Guiyang, 3Department of Pediatric Dentistry, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 4Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, China Abstract: Due to the widespread applications of zinc oxide nanoparticles (ZnO NPs), the potential exposure of workers, consumers, and scientists to these particles has increased. This potential for exposure has attracted extensive attention in the science community. Many studies have examined the toxicological profile of ZnO NPs in the immune system, digestive system, however, information regarding the toxicity of ZnO NPs in the nervous system is scarce. In this study, we detected the cytotoxicity of two types of ZnO NPs of various sizes – ZnOa NPs and ZnOb NPs – and we characterized the shedding ability of zinc ions within culture medium and the cytoplasm. We found that reactive oxygen species played a crucial role in ZnO NP-induced cytotoxicity, likely because zinc ions were leached from ZnO NPs. Apoptosis and cytoskeleton changes were also toxic responses induced by the ZnO NPs, and ZnOb NPs induced more significant toxic responses than ZnOa NPs in SHSY5Y cells. In conclusion, ZnO NPs induced toxic responses in SHSY5Y cells in a size-dependent manner, which can probably be attributed to their ion-shedding ability. Keywords: zinc oxide, nanoparticles, SHSY5Y cells, reactive oxygen species, apoptosis, cell cytoskeletonLiu JKang YYin SSong BWei LChen LShao LDove Medical PressarticleZinc oxidenanoparticlesSH-SY5Y cellsreactive oxygen speciesapoptosiscell cytoskeletonMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 8085-8099 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zinc oxide nanoparticles SH-SY5Y cells reactive oxygen species apoptosis cell cytoskeleton Medicine (General) R5-920
spellingShingle	Zinc oxide nanoparticles SH-SY5Y cells reactive oxygen species apoptosis cell cytoskeleton Medicine (General) R5-920 Liu J Kang Y Yin S Song B Wei L Chen L Shao L Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner
description	Jia Liu,1 Yiyuan Kang,1 Suhan Yin,1 Bin Song,2 Limin Wei,3 Liangjiao Chen,4 Longquan Shao1 1Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, 2Department of Stomatology, Guizhou Provincial People’s Hospital, Guiyang, 3Department of Pediatric Dentistry, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 4Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, China Abstract: Due to the widespread applications of zinc oxide nanoparticles (ZnO NPs), the potential exposure of workers, consumers, and scientists to these particles has increased. This potential for exposure has attracted extensive attention in the science community. Many studies have examined the toxicological profile of ZnO NPs in the immune system, digestive system, however, information regarding the toxicity of ZnO NPs in the nervous system is scarce. In this study, we detected the cytotoxicity of two types of ZnO NPs of various sizes – ZnOa NPs and ZnOb NPs – and we characterized the shedding ability of zinc ions within culture medium and the cytoplasm. We found that reactive oxygen species played a crucial role in ZnO NP-induced cytotoxicity, likely because zinc ions were leached from ZnO NPs. Apoptosis and cytoskeleton changes were also toxic responses induced by the ZnO NPs, and ZnOb NPs induced more significant toxic responses than ZnOa NPs in SHSY5Y cells. In conclusion, ZnO NPs induced toxic responses in SHSY5Y cells in a size-dependent manner, which can probably be attributed to their ion-shedding ability. Keywords: zinc oxide, nanoparticles, SHSY5Y cells, reactive oxygen species, apoptosis, cell cytoskeleton
format	article
author	Liu J Kang Y Yin S Song B Wei L Chen L Shao L
author_facet	Liu J Kang Y Yin S Song B Wei L Chen L Shao L
author_sort	Liu J
title	Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner
title_short	Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner
title_full	Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner
title_fullStr	Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner
title_full_unstemmed	Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner
title_sort	zinc oxide nanoparticles induce toxic responses in human neuroblastoma shsy5y cells in a size-dependent manner
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/9a88b72ab94e4911ac131115da7c85b0
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Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y cells in a size-dependent manner

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