Prognostic Significance of Alternative Splicing Genes in Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma

Xiaoyu Wang,&ast; Weichun Tang,&ast; Yilin Lu, Jun You, Yun Han, Yanli Zheng Department of Obstetrics and Gynecology, Nantong First People’s Hospital, Nantong, Jiangsu, 226001, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Yanli ZhengDepartm...

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Autores principales:	Wang X, Tang W, Lu Y, You J, Han Y, Zheng Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	cervical squamous cell carcinoma and endocervical adenocarcinoma (cesc) alternative splicing (as) prognosis tcga Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9a8be4877d544b2d85042f4185518494
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spelling	oai:doaj.org-article:9a8be4877d544b2d85042f41855184942021-11-09T18:40:34ZPrognostic Significance of Alternative Splicing Genes in Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma1178-7074https://doaj.org/article/9a8be4877d544b2d85042f41855184942021-11-01T00:00:00Zhttps://www.dovepress.com/prognostic-significance-of-alternative-splicing-genes-in-cervical-squa-peer-reviewed-fulltext-article-IJGMhttps://doaj.org/toc/1178-7074Xiaoyu Wang,&ast; Weichun Tang,&ast; Yilin Lu, Jun You, Yun Han, Yanli Zheng Department of Obstetrics and Gynecology, Nantong First People’s Hospital, Nantong, Jiangsu, 226001, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Yanli ZhengDepartment of Obstetrics and Gynecology, Nantong First People’s Hospital, Nantong, Jiangsu, 226001, People’s Republic of ChinaTel/Fax +86-513-85051875Email gaoshan1189@sina.comBackground: Alternative splicing (AS) acts on many tumors and its relationship with cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) needs to be researched.Methods: RNA sequencing data and clinical information of CESC cohorts were obtained from the Cancer Genome Atlas (TCGA) and SpliceSeq was used to analyze the splicing profile of mRNA in CESC. UpSetR displayed the intersections among AS events and univariate analysis chose survival-associated AS and splicing factor (SF) genes. Functional analysis was operated on Enrichr, STRING database and MCODE analysis were used to evaluate protein–protein interaction (PPI) information. LASSO and multivariate analysis constructed prognostic model and risk analysis of tumor infiltrating immune cells was also conducted.Results: A total of 402 AS-generated genes were found to be associated with CESC prognosis. Functional analysis showed that Golgi to lysosome transport was enriched. PPI network suggested that UBA52 was most functional. Dendritic cells activated, dendritic cells resting, macrophages M0, mast cells resting, T cells CD4 memory activated and T cells CD8 were most correlative with the risk score.Conclusion: SFs and AS events can directly or indirectly affect the prognosis of CESC patients and this study identified SNRPA and CELF2 as two CESC-engaged SFs.Keywords: cervical squamous cell carcinoma and endocervical adenocarcinoma, CESC, alternative splicing, AS, prognosis, TCGAWang XTang WLu YYou JHan YZheng YDove Medical Pressarticlecervical squamous cell carcinoma and endocervical adenocarcinoma (cesc)alternative splicing (as)prognosistcgaMedicine (General)R5-920ENInternational Journal of General Medicine, Vol Volume 14, Pp 7933-7949 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cervical squamous cell carcinoma and endocervical adenocarcinoma (cesc) alternative splicing (as) prognosis tcga Medicine (General) R5-920
spellingShingle	cervical squamous cell carcinoma and endocervical adenocarcinoma (cesc) alternative splicing (as) prognosis tcga Medicine (General) R5-920 Wang X Tang W Lu Y You J Han Y Zheng Y Prognostic Significance of Alternative Splicing Genes in Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma
description	Xiaoyu Wang,&ast; Weichun Tang,&ast; Yilin Lu, Jun You, Yun Han, Yanli Zheng Department of Obstetrics and Gynecology, Nantong First People’s Hospital, Nantong, Jiangsu, 226001, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Yanli ZhengDepartment of Obstetrics and Gynecology, Nantong First People’s Hospital, Nantong, Jiangsu, 226001, People’s Republic of ChinaTel/Fax +86-513-85051875Email gaoshan1189@sina.comBackground: Alternative splicing (AS) acts on many tumors and its relationship with cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) needs to be researched.Methods: RNA sequencing data and clinical information of CESC cohorts were obtained from the Cancer Genome Atlas (TCGA) and SpliceSeq was used to analyze the splicing profile of mRNA in CESC. UpSetR displayed the intersections among AS events and univariate analysis chose survival-associated AS and splicing factor (SF) genes. Functional analysis was operated on Enrichr, STRING database and MCODE analysis were used to evaluate protein–protein interaction (PPI) information. LASSO and multivariate analysis constructed prognostic model and risk analysis of tumor infiltrating immune cells was also conducted.Results: A total of 402 AS-generated genes were found to be associated with CESC prognosis. Functional analysis showed that Golgi to lysosome transport was enriched. PPI network suggested that UBA52 was most functional. Dendritic cells activated, dendritic cells resting, macrophages M0, mast cells resting, T cells CD4 memory activated and T cells CD8 were most correlative with the risk score.Conclusion: SFs and AS events can directly or indirectly affect the prognosis of CESC patients and this study identified SNRPA and CELF2 as two CESC-engaged SFs.Keywords: cervical squamous cell carcinoma and endocervical adenocarcinoma, CESC, alternative splicing, AS, prognosis, TCGA
format	article
author	Wang X Tang W Lu Y You J Han Y Zheng Y
author_facet	Wang X Tang W Lu Y You J Han Y Zheng Y
author_sort	Wang X
title	Prognostic Significance of Alternative Splicing Genes in Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma
title_short	Prognostic Significance of Alternative Splicing Genes in Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma
title_full	Prognostic Significance of Alternative Splicing Genes in Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma
title_fullStr	Prognostic Significance of Alternative Splicing Genes in Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma
title_full_unstemmed	Prognostic Significance of Alternative Splicing Genes in Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma
title_sort	prognostic significance of alternative splicing genes in cervical squamous cell carcinoma and endocervical adenocarcinoma
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/9a8be4877d544b2d85042f4185518494
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_version_	1718440865322500096

Prognostic Significance of Alternative Splicing Genes in Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma

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