Knockdown of lncRNA MIR31HG inhibits adipocyte differentiation of human adipose-derived stem cells via histone modification of FABP4

Abstract Adipogenesis plays an important role in the regulation of whole-body energy homeostasis and is inextricably related to obesity. Several studies have highlighted the relevance of microRNAs in adipocyte differentiation, but the contributions of long non-coding RNAs (lncRNAs) are still largely...

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Autores principales:	Yiping Huang, Chanyuan Jin, Yunfei Zheng, Xiaobei Li, Shan Zhang, Yixin Zhang, Lingfei Jia, Weiran Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9a8c8cbe3f3742c5b147dcc48607fcf7
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spelling	oai:doaj.org-article:9a8c8cbe3f3742c5b147dcc48607fcf72021-12-02T15:06:23ZKnockdown of lncRNA MIR31HG inhibits adipocyte differentiation of human adipose-derived stem cells via histone modification of FABP410.1038/s41598-017-08131-62045-2322https://doaj.org/article/9a8c8cbe3f3742c5b147dcc48607fcf72017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08131-6https://doaj.org/toc/2045-2322Abstract Adipogenesis plays an important role in the regulation of whole-body energy homeostasis and is inextricably related to obesity. Several studies have highlighted the relevance of microRNAs in adipocyte differentiation, but the contributions of long non-coding RNAs (lncRNAs) are still largely uncharacterized. Here, we determined that lncRNA MIR31HG is related to adipocyte lineage commitment. We demonstrated that knockdown of MIR31HG inhibited adipocyte differentiation, whereas overexpression of MIR31HG promoted adipogenesis in vitro and in vivo. Furthermore, inhibition of MIR31HG reduced the enrichment of active histone markers, histone H3 lysine 4 trimethylation (H3K4me3) and acetylation (AcH3), in the promoter of the adipogenic-related gene, fatty acid binding protein 4 (FABP4), leading to suppression of its expression and adipogenesis. These results provide new insights into the molecular mechanisms of MIR31HG in terms of adipogenesis and may have implications for obesity and associated disorders.Yiping HuangChanyuan JinYunfei ZhengXiaobei LiShan ZhangYixin ZhangLingfei JiaWeiran LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yiping Huang Chanyuan Jin Yunfei Zheng Xiaobei Li Shan Zhang Yixin Zhang Lingfei Jia Weiran Li Knockdown of lncRNA MIR31HG inhibits adipocyte differentiation of human adipose-derived stem cells via histone modification of FABP4
description	Abstract Adipogenesis plays an important role in the regulation of whole-body energy homeostasis and is inextricably related to obesity. Several studies have highlighted the relevance of microRNAs in adipocyte differentiation, but the contributions of long non-coding RNAs (lncRNAs) are still largely uncharacterized. Here, we determined that lncRNA MIR31HG is related to adipocyte lineage commitment. We demonstrated that knockdown of MIR31HG inhibited adipocyte differentiation, whereas overexpression of MIR31HG promoted adipogenesis in vitro and in vivo. Furthermore, inhibition of MIR31HG reduced the enrichment of active histone markers, histone H3 lysine 4 trimethylation (H3K4me3) and acetylation (AcH3), in the promoter of the adipogenic-related gene, fatty acid binding protein 4 (FABP4), leading to suppression of its expression and adipogenesis. These results provide new insights into the molecular mechanisms of MIR31HG in terms of adipogenesis and may have implications for obesity and associated disorders.
format	article
author	Yiping Huang Chanyuan Jin Yunfei Zheng Xiaobei Li Shan Zhang Yixin Zhang Lingfei Jia Weiran Li
author_facet	Yiping Huang Chanyuan Jin Yunfei Zheng Xiaobei Li Shan Zhang Yixin Zhang Lingfei Jia Weiran Li
author_sort	Yiping Huang
title	Knockdown of lncRNA MIR31HG inhibits adipocyte differentiation of human adipose-derived stem cells via histone modification of FABP4
title_short	Knockdown of lncRNA MIR31HG inhibits adipocyte differentiation of human adipose-derived stem cells via histone modification of FABP4
title_full	Knockdown of lncRNA MIR31HG inhibits adipocyte differentiation of human adipose-derived stem cells via histone modification of FABP4
title_fullStr	Knockdown of lncRNA MIR31HG inhibits adipocyte differentiation of human adipose-derived stem cells via histone modification of FABP4
title_full_unstemmed	Knockdown of lncRNA MIR31HG inhibits adipocyte differentiation of human adipose-derived stem cells via histone modification of FABP4
title_sort	knockdown of lncrna mir31hg inhibits adipocyte differentiation of human adipose-derived stem cells via histone modification of fabp4
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9a8c8cbe3f3742c5b147dcc48607fcf7
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Knockdown of lncRNA MIR31HG inhibits adipocyte differentiation of human adipose-derived stem cells via histone modification of FABP4

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