Gene polymorphisms of IL-1β (C511T), IL-17A (G197A), IL-12B (A1188C), TNFα (G308A) and IL-4 (C589T) associated with threat of early reproductive losses

Prevalence of threatened miscarriage is 16-25% of all pregnancies. The symptomatics of the threatened miscarriage in the first trimester may develop due to conversion of initial inflammatory reaction that disturbs the intersystemic and local interactions in the endometrium, followed by placental ins...

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Autores principales:	E. A. Tatarkova, A. R. Tuguz, A. A. Tsikunib, D. S. Shumilov, I. V. Smolkov, K. A. Rudenko, D. V. Muzhenya
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2020
Materias:	threatened miscarriage snps of cytokine genes il-17a (g197a rs2275913) il-1β (с511т rs16944) il-4 (c589t rs2243250) tnfα (g308a rs1800629) il-12b (a1188c rs3212227) Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/9a8ef8f989da432b8bcea26af7101118
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spelling	oai:doaj.org-article:9a8ef8f989da432b8bcea26af71011182021-11-18T08:03:49ZGene polymorphisms of IL-1β (C511T), IL-17A (G197A), IL-12B (A1188C), TNFα (G308A) and IL-4 (C589T) associated with threat of early reproductive losses1563-06252313-741X10.15789/1563-0625-2019-6-1179-1186https://doaj.org/article/9a8ef8f989da432b8bcea26af71011182020-01-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1741https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XPrevalence of threatened miscarriage is 16-25% of all pregnancies. The symptomatics of the threatened miscarriage in the first trimester may develop due to conversion of initial inflammatory reaction that disturbs the intersystemic and local interactions in the endometrium, followed by placental insufficiency, intrauterine fetal affection and spontaneous abortion. The aim of our work was to study the association between single nucleotide polymorphisms (SNPs) of some cytokine genes, e.g., IL-1β (C511T, rs16944), IL-17A (G197A, rs2275913), IL-12B (A1188C, rs3212227), TNFα (G308A, rs1800629), and IL-4 (C589T, rs2243250) and the risk of early reproductive losses among residents of Adyghe Republic (RA). The work was carried out at the Immunogenetic Laboratory of our Research Institute of Complex Problems. The allelic variants of cytokine genes were detected by SNP-method in 106 samples of genomic DNA in women with the threatened abortion in 1st trimester (n = 58) and the uncomplicated gestation (n = 48). SNP-typing of polymorphic variants of cytokine genes IL-1β, IL-17A, IL-12B, TNFα and IL-4 was carried out by PCR (polymerase chain reaction) with allele-specific primers and electrophoretic detection of results on test systems of (Litech, Moscow). Statistical analysis of experimental data was carried out by SPSS Statistical program 17.0. The correspondence of SNP distributions to expected values at Hardy—Weinberg equilibrium and comparison of allelic variants/ genotypes frequencies were performed using the χ2 criterion (Chi-square with Yates correction), odd ratios (OR) detected at significance level p < 0.05 and 95% confidence interval (95% CI). Reliability of the differences for the SNP frequencies for small samples was evaluated using the Fisher’s exact criterion. Heterozygous variant (C511T; OR = 3.46; 95% Cl: 1.04-11.54) and homozygous “mutant” genotypes (T511T; OR = 5.71; 95% Cl: 1.12-29.09) of the main proinflammatory IL-1β was significantly associated (p < 0.05) with the risk of developing threatening miscarriage in the Adygea residents. The -511Т allele of IL-1β gene, and -1188C variant of IL-12B gene increase the risk of the early termination of pregnancy, respectively, 5.8-fold (95% Cl: 2.4213.92; p = 0.00004), and 2.97-fold (95% Cl: 1.23-7.19; p = 0.01). The “mutant” -511T allelic variant of the IL-1 p gene is associated with the risk of developing a symptome complex of threatening miscarriage in Russian ethnic group (p = 0.0001; OR = 14.09), and in Adygea ethnic group (p = 0.02; OR = 8.17), which is almost undetectable in women with normal pregnancy in the first trimester. Thus, only С511Т (rs16944) in IL-1β gene and A1188C (rs3212227) in IL-12B gene of the five typed cytokine genes may be used as marker polymorphisms of gestational distress for the women in Adygea.E. A. TatarkovaA. R. TuguzA. A. TsikunibD. S. ShumilovI. V. SmolkovK. A. RudenkoD. V. MuzhenyaSPb RAACIarticlethreatened miscarriagesnps of cytokine genesil-17a (g197ars2275913)il-1β (с511тrs16944)il-4 (c589trs2243250)tnfα (g308ars1800629)il-12b (a1188crs3212227)Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 6, Pp 1179-1186 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	threatened miscarriage snps of cytokine genes il-17a (g197a rs2275913) il-1β (с511т rs16944) il-4 (c589t rs2243250) tnfα (g308a rs1800629) il-12b (a1188c rs3212227) Immunologic diseases. Allergy RC581-607
spellingShingle	threatened miscarriage snps of cytokine genes il-17a (g197a rs2275913) il-1β (с511т rs16944) il-4 (c589t rs2243250) tnfα (g308a rs1800629) il-12b (a1188c rs3212227) Immunologic diseases. Allergy RC581-607 E. A. Tatarkova A. R. Tuguz A. A. Tsikunib D. S. Shumilov I. V. Smolkov K. A. Rudenko D. V. Muzhenya Gene polymorphisms of IL-1β (C511T), IL-17A (G197A), IL-12B (A1188C), TNFα (G308A) and IL-4 (C589T) associated with threat of early reproductive losses
description	Prevalence of threatened miscarriage is 16-25% of all pregnancies. The symptomatics of the threatened miscarriage in the first trimester may develop due to conversion of initial inflammatory reaction that disturbs the intersystemic and local interactions in the endometrium, followed by placental insufficiency, intrauterine fetal affection and spontaneous abortion. The aim of our work was to study the association between single nucleotide polymorphisms (SNPs) of some cytokine genes, e.g., IL-1β (C511T, rs16944), IL-17A (G197A, rs2275913), IL-12B (A1188C, rs3212227), TNFα (G308A, rs1800629), and IL-4 (C589T, rs2243250) and the risk of early reproductive losses among residents of Adyghe Republic (RA). The work was carried out at the Immunogenetic Laboratory of our Research Institute of Complex Problems. The allelic variants of cytokine genes were detected by SNP-method in 106 samples of genomic DNA in women with the threatened abortion in 1st trimester (n = 58) and the uncomplicated gestation (n = 48). SNP-typing of polymorphic variants of cytokine genes IL-1β, IL-17A, IL-12B, TNFα and IL-4 was carried out by PCR (polymerase chain reaction) with allele-specific primers and electrophoretic detection of results on test systems of (Litech, Moscow). Statistical analysis of experimental data was carried out by SPSS Statistical program 17.0. The correspondence of SNP distributions to expected values at Hardy—Weinberg equilibrium and comparison of allelic variants/ genotypes frequencies were performed using the χ2 criterion (Chi-square with Yates correction), odd ratios (OR) detected at significance level p < 0.05 and 95% confidence interval (95% CI). Reliability of the differences for the SNP frequencies for small samples was evaluated using the Fisher’s exact criterion. Heterozygous variant (C511T; OR = 3.46; 95% Cl: 1.04-11.54) and homozygous “mutant” genotypes (T511T; OR = 5.71; 95% Cl: 1.12-29.09) of the main proinflammatory IL-1β was significantly associated (p < 0.05) with the risk of developing threatening miscarriage in the Adygea residents. The -511Т allele of IL-1β gene, and -1188C variant of IL-12B gene increase the risk of the early termination of pregnancy, respectively, 5.8-fold (95% Cl: 2.4213.92; p = 0.00004), and 2.97-fold (95% Cl: 1.23-7.19; p = 0.01). The “mutant” -511T allelic variant of the IL-1 p gene is associated with the risk of developing a symptome complex of threatening miscarriage in Russian ethnic group (p = 0.0001; OR = 14.09), and in Adygea ethnic group (p = 0.02; OR = 8.17), which is almost undetectable in women with normal pregnancy in the first trimester. Thus, only С511Т (rs16944) in IL-1β gene and A1188C (rs3212227) in IL-12B gene of the five typed cytokine genes may be used as marker polymorphisms of gestational distress for the women in Adygea.
format	article
author	E. A. Tatarkova A. R. Tuguz A. A. Tsikunib D. S. Shumilov I. V. Smolkov K. A. Rudenko D. V. Muzhenya
author_facet	E. A. Tatarkova A. R. Tuguz A. A. Tsikunib D. S. Shumilov I. V. Smolkov K. A. Rudenko D. V. Muzhenya
author_sort	E. A. Tatarkova
title	Gene polymorphisms of IL-1β (C511T), IL-17A (G197A), IL-12B (A1188C), TNFα (G308A) and IL-4 (C589T) associated with threat of early reproductive losses
title_short	Gene polymorphisms of IL-1β (C511T), IL-17A (G197A), IL-12B (A1188C), TNFα (G308A) and IL-4 (C589T) associated with threat of early reproductive losses
title_full	Gene polymorphisms of IL-1β (C511T), IL-17A (G197A), IL-12B (A1188C), TNFα (G308A) and IL-4 (C589T) associated with threat of early reproductive losses
title_fullStr	Gene polymorphisms of IL-1β (C511T), IL-17A (G197A), IL-12B (A1188C), TNFα (G308A) and IL-4 (C589T) associated with threat of early reproductive losses
title_full_unstemmed	Gene polymorphisms of IL-1β (C511T), IL-17A (G197A), IL-12B (A1188C), TNFα (G308A) and IL-4 (C589T) associated with threat of early reproductive losses
title_sort	gene polymorphisms of il-1β (c511t), il-17a (g197a), il-12b (a1188c), tnfα (g308a) and il-4 (c589t) associated with threat of early reproductive losses
publisher	SPb RAACI
publishDate	2020
url	https://doaj.org/article/9a8ef8f989da432b8bcea26af7101118
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Gene polymorphisms of IL-1β (C511T), IL-17A (G197A), IL-12B (A1188C), TNFα (G308A) and IL-4 (C589T) associated with threat of early reproductive losses

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