SHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis.

SHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis.

Allergic inflammation and severe allergic reactions (anaphylaxis) are important in allergen induced diseases. Bacterial products such as lipopolysaccharide (LPS) are ubiquitous and can facilitate allergen induced Th2 immune responses. Phosphatase SHP-1 is critical in regulating immunological homeost...

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Autores principales: Li Zhou, Sun Young Oh, Yuqi Zhou, Baojun Yuan, Fan Wu, Min Hee Oh, Yefu Wang, Cliff Takemoto, Nico Van Rooijen, Tao Zheng, Zhou Zhu
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2013
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/9a9aaf580e9b45fc822c7b44f068abc8
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spelling oai:doaj.org-article:9a9aaf580e9b45fc822c7b44f068abc82021-11-18T07:58:51ZSHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis.1932-620310.1371/journal.pone.0055763https://doaj.org/article/9a9aaf580e9b45fc822c7b44f068abc82013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23390550/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Allergic inflammation and severe allergic reactions (anaphylaxis) are important in allergen induced diseases. Bacterial products such as lipopolysaccharide (LPS) are ubiquitous and can facilitate allergen induced Th2 immune responses. Phosphatase SHP-1 is critical in regulating immunological homeostasis and in allergen induced Th2 immune responses in the lung. However, the mechanisms underlying the initiation of allergic inflammation and allergen induced anaphylaxis are still not completely elucidated and it is unclear whether SHP-1 plays any role in LPS-induced airway inflammation and in allergen-induced anaphylaxis. In this study we tested the hypothesis that phosphatase SHP-1 plays an important role in allergic inflammation and anaphylaxis and determined whether its effects are through regulation of mast cell functions. SHP-1 deficient (mev/+ and mev/mev) and mast cell deficient (Kit(W-sh)) mice were examined in their responses to LPS airway stimulation and to ovalbumin (OVA) allergen induced systemic anaphylaxis. Compared to wild type mice, mev/+ mice had significantly enhanced LPS induced airway inflammation and OVA induced anaphylactic responses, including hypothermia and clinical symptoms. These changes were mast cell dependent as Kit(W-sh) mice had reduced responses whereas adoptive transfer of mast cells restored the responses. However, T and B cells were not involved and macrophages did not play a significant role in LPS induced airway inflammation. Interestingly, basophil differentiation from SHP-1 deficient bone marrow cells was significantly reduced. These findings provided evidence that through regulation of mast cell functions SHP-1 plays a critical role as a negative regulator in allergic inflammation and in allergen induced anaphylaxis. In addition, SHP-1 seems to be required for normal basophil development.Li ZhouSun Young OhYuqi ZhouBaojun YuanFan WuMin Hee OhYefu WangCliff TakemotoNico Van RooijenTao ZhengZhou ZhuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e55763 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Li Zhou
Sun Young Oh
Yuqi Zhou
Baojun Yuan
Fan Wu
Min Hee Oh
Yefu Wang
Cliff Takemoto
Nico Van Rooijen
Tao Zheng
Zhou Zhu
SHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis.
description Allergic inflammation and severe allergic reactions (anaphylaxis) are important in allergen induced diseases. Bacterial products such as lipopolysaccharide (LPS) are ubiquitous and can facilitate allergen induced Th2 immune responses. Phosphatase SHP-1 is critical in regulating immunological homeostasis and in allergen induced Th2 immune responses in the lung. However, the mechanisms underlying the initiation of allergic inflammation and allergen induced anaphylaxis are still not completely elucidated and it is unclear whether SHP-1 plays any role in LPS-induced airway inflammation and in allergen-induced anaphylaxis. In this study we tested the hypothesis that phosphatase SHP-1 plays an important role in allergic inflammation and anaphylaxis and determined whether its effects are through regulation of mast cell functions. SHP-1 deficient (mev/+ and mev/mev) and mast cell deficient (Kit(W-sh)) mice were examined in their responses to LPS airway stimulation and to ovalbumin (OVA) allergen induced systemic anaphylaxis. Compared to wild type mice, mev/+ mice had significantly enhanced LPS induced airway inflammation and OVA induced anaphylactic responses, including hypothermia and clinical symptoms. These changes were mast cell dependent as Kit(W-sh) mice had reduced responses whereas adoptive transfer of mast cells restored the responses. However, T and B cells were not involved and macrophages did not play a significant role in LPS induced airway inflammation. Interestingly, basophil differentiation from SHP-1 deficient bone marrow cells was significantly reduced. These findings provided evidence that through regulation of mast cell functions SHP-1 plays a critical role as a negative regulator in allergic inflammation and in allergen induced anaphylaxis. In addition, SHP-1 seems to be required for normal basophil development.
format article
author Li Zhou
Sun Young Oh
Yuqi Zhou
Baojun Yuan
Fan Wu
Min Hee Oh
Yefu Wang
Cliff Takemoto
Nico Van Rooijen
Tao Zheng
Zhou Zhu
author_facet Li Zhou
Sun Young Oh
Yuqi Zhou
Baojun Yuan
Fan Wu
Min Hee Oh
Yefu Wang
Cliff Takemoto
Nico Van Rooijen
Tao Zheng
Zhou Zhu
author_sort Li Zhou
title SHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis.
title_short SHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis.
title_full SHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis.
title_fullStr SHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis.
title_full_unstemmed SHP-1 regulation of mast cell function in allergic inflammation and anaphylaxis.
title_sort shp-1 regulation of mast cell function in allergic inflammation and anaphylaxis.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/9a9aaf580e9b45fc822c7b44f068abc8
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AT yuqizhou shp1regulationofmastcellfunctioninallergicinflammationandanaphylaxis
AT baojunyuan shp1regulationofmastcellfunctioninallergicinflammationandanaphylaxis
AT fanwu shp1regulationofmastcellfunctioninallergicinflammationandanaphylaxis
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AT nicovanrooijen shp1regulationofmastcellfunctioninallergicinflammationandanaphylaxis
AT taozheng shp1regulationofmastcellfunctioninallergicinflammationandanaphylaxis
AT zhouzhu shp1regulationofmastcellfunctioninallergicinflammationandanaphylaxis
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