MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response

Viral infections seriously affect the health of organisms including humans. Now, more and more researchers believe that microRNAs (miRNAs), one of the members of the non-coding RNA family, play significant roles in cell biological function, disease occurrence, and immunotherapy. However, the roles o...

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Autores principales:	Pin-Hong Li, Li-Qun Wang, Jia-Yang He, Xiang-Long Zhu, Wei Huang, Shao-Wen Wang, Qi-Wei Qin, Hong-Yan Sun
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	miR-124 Epinephelus coioides SGIV viral replication immune response Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/9aa2130dd3334dd6a30c7340fc7f7ede
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spelling	oai:doaj.org-article:9aa2130dd3334dd6a30c7340fc7f7ede2021-11-10T06:29:21ZMicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response1664-322410.3389/fimmu.2021.767813https://doaj.org/article/9aa2130dd3334dd6a30c7340fc7f7ede2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.767813/fullhttps://doaj.org/toc/1664-3224Viral infections seriously affect the health of organisms including humans. Now, more and more researchers believe that microRNAs (miRNAs), one of the members of the non-coding RNA family, play significant roles in cell biological function, disease occurrence, and immunotherapy. However, the roles of miRNAs in virus infection (entry and replication) and cellular immune response remain poorly understood, especially in low vertebrate fish. In this study, based on the established virus-cell infection model, Singapore grouper iridovirus (SGIV)-infected cells were used to explore the roles of miR-124 of Epinephelus coioides, an economically mariculture fish in southern China and Southeast Asia, in viral infection and host immune responses. The expression level of E. coioides miR-124 was significantly upregulated after SGIV infection; miR-124 cannot significantly affect the entry of SGIV, but the upregulated miR-124 could significantly promote the SGIV-induced cytopathic effects (CPEs), the viral titer, and the expressions of viral genes. The target genes of miR-124 were JNK3/p38α mitogen-activated protein kinase (MAPK). Overexpression of miR-124 could dramatically inhibit the activation of NF-κB/activating protein-1 (AP-1), the transcription of proinflammatory factors, caspase-9/3, and the cell apoptosis. And opposite results happen when the expression of miR-124 was inhibited. The results suggest that E. coioides miR-124 could promote viral replication and negatively regulate host immune response by targeting JNK3/p38α MAPK, which furthers our understanding of virus and host immune interactions.Pin-Hong LiLi-Qun WangJia-Yang HeXiang-Long ZhuWei HuangShao-Wen WangQi-Wei QinQi-Wei QinQi-Wei QinHong-Yan SunFrontiers Media S.A.articlemiR-124Epinephelus coioidesSGIVviral replicationimmune responseImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	miR-124 Epinephelus coioides SGIV viral replication immune response Immunologic diseases. Allergy RC581-607
spellingShingle	miR-124 Epinephelus coioides SGIV viral replication immune response Immunologic diseases. Allergy RC581-607 Pin-Hong Li Li-Qun Wang Jia-Yang He Xiang-Long Zhu Wei Huang Shao-Wen Wang Qi-Wei Qin Qi-Wei Qin Qi-Wei Qin Hong-Yan Sun MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
description	Viral infections seriously affect the health of organisms including humans. Now, more and more researchers believe that microRNAs (miRNAs), one of the members of the non-coding RNA family, play significant roles in cell biological function, disease occurrence, and immunotherapy. However, the roles of miRNAs in virus infection (entry and replication) and cellular immune response remain poorly understood, especially in low vertebrate fish. In this study, based on the established virus-cell infection model, Singapore grouper iridovirus (SGIV)-infected cells were used to explore the roles of miR-124 of Epinephelus coioides, an economically mariculture fish in southern China and Southeast Asia, in viral infection and host immune responses. The expression level of E. coioides miR-124 was significantly upregulated after SGIV infection; miR-124 cannot significantly affect the entry of SGIV, but the upregulated miR-124 could significantly promote the SGIV-induced cytopathic effects (CPEs), the viral titer, and the expressions of viral genes. The target genes of miR-124 were JNK3/p38α mitogen-activated protein kinase (MAPK). Overexpression of miR-124 could dramatically inhibit the activation of NF-κB/activating protein-1 (AP-1), the transcription of proinflammatory factors, caspase-9/3, and the cell apoptosis. And opposite results happen when the expression of miR-124 was inhibited. The results suggest that E. coioides miR-124 could promote viral replication and negatively regulate host immune response by targeting JNK3/p38α MAPK, which furthers our understanding of virus and host immune interactions.
format	article
author	Pin-Hong Li Li-Qun Wang Jia-Yang He Xiang-Long Zhu Wei Huang Shao-Wen Wang Qi-Wei Qin Qi-Wei Qin Qi-Wei Qin Hong-Yan Sun
author_facet	Pin-Hong Li Li-Qun Wang Jia-Yang He Xiang-Long Zhu Wei Huang Shao-Wen Wang Qi-Wei Qin Qi-Wei Qin Qi-Wei Qin Hong-Yan Sun
author_sort	Pin-Hong Li
title	MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
title_short	MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
title_full	MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
title_fullStr	MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
title_full_unstemmed	MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
title_sort	microrna-124 promotes singapore grouper iridovirus replication and negatively regulates innate immune response
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9aa2130dd3334dd6a30c7340fc7f7ede
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MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response

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