Self-sampling of capillary blood for SARS-CoV-2 serology

Abstract Serological testing is emerging as a powerful tool to progress our understanding of COVID-19 exposure, transmission and immune response. Large-scale testing is limited by the need for in-person blood collection by staff trained in venepuncture, and the limited sensitivity of lateral flow te...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lottie Brown, Rachel L. Byrne, Alice Fraser, Sophie I. Owen, Ana I. Cubas-Atienzar, Christopher T. Williams, Grant A. Kay, Luis E. Cuevas, Joseph R. A. Fitchett, Tom Fletcher, Gala Garrod, Konstantina Kontogianni, Sanjeev Krishna, Stefanie Menzies, Tim Planche, Chris Sainter, Henry M. Staines, Lance Turtle, Emily R. Adams
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9aa2f5d1ea794a83af7a2a1c7036d7bf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9aa2f5d1ea794a83af7a2a1c7036d7bf
record_format	dspace
spelling	oai:doaj.org-article:9aa2f5d1ea794a83af7a2a1c7036d7bf2021-12-02T14:37:46ZSelf-sampling of capillary blood for SARS-CoV-2 serology10.1038/s41598-021-86008-52045-2322https://doaj.org/article/9aa2f5d1ea794a83af7a2a1c7036d7bf2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86008-5https://doaj.org/toc/2045-2322Abstract Serological testing is emerging as a powerful tool to progress our understanding of COVID-19 exposure, transmission and immune response. Large-scale testing is limited by the need for in-person blood collection by staff trained in venepuncture, and the limited sensitivity of lateral flow tests. Capillary blood self-sampling and postage to laboratories for analysis could provide a reliable alternative. Two-hundred and nine matched venous and capillary blood samples were obtained from thirty nine participants and analysed using a COVID-19 IgG ELISA to detect antibodies against SARS-CoV-2. Thirty eight out of thirty nine participants were able to self-collect an adequate sample of capillary blood (≥ 50 µl). Using plasma from venous blood collected in lithium heparin as the reference standard, matched capillary blood samples, collected in lithium heparin-treated tubes and on filter paper as dried blood spots, achieved a Cohen’s kappa coefficient of > 0.88 (near-perfect agreement, 95% CI 0.738–1.000). Storage of capillary blood at room temperature for up to 7 days post sampling did not affect concordance. Our results indicate that capillary blood self-sampling is a reliable and feasible alternative to venepuncture for serological assessment in COVID-19.Lottie BrownRachel L. ByrneAlice FraserSophie I. OwenAna I. Cubas-AtienzarChristopher T. WilliamsGrant A. KayLuis E. CuevasJoseph R. A. FitchettTom FletcherGala GarrodKonstantina KontogianniSanjeev KrishnaStefanie MenziesTim PlancheChris SainterHenry M. StainesLance TurtleEmily R. AdamsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lottie Brown Rachel L. Byrne Alice Fraser Sophie I. Owen Ana I. Cubas-Atienzar Christopher T. Williams Grant A. Kay Luis E. Cuevas Joseph R. A. Fitchett Tom Fletcher Gala Garrod Konstantina Kontogianni Sanjeev Krishna Stefanie Menzies Tim Planche Chris Sainter Henry M. Staines Lance Turtle Emily R. Adams Self-sampling of capillary blood for SARS-CoV-2 serology
description	Abstract Serological testing is emerging as a powerful tool to progress our understanding of COVID-19 exposure, transmission and immune response. Large-scale testing is limited by the need for in-person blood collection by staff trained in venepuncture, and the limited sensitivity of lateral flow tests. Capillary blood self-sampling and postage to laboratories for analysis could provide a reliable alternative. Two-hundred and nine matched venous and capillary blood samples were obtained from thirty nine participants and analysed using a COVID-19 IgG ELISA to detect antibodies against SARS-CoV-2. Thirty eight out of thirty nine participants were able to self-collect an adequate sample of capillary blood (≥ 50 µl). Using plasma from venous blood collected in lithium heparin as the reference standard, matched capillary blood samples, collected in lithium heparin-treated tubes and on filter paper as dried blood spots, achieved a Cohen’s kappa coefficient of > 0.88 (near-perfect agreement, 95% CI 0.738–1.000). Storage of capillary blood at room temperature for up to 7 days post sampling did not affect concordance. Our results indicate that capillary blood self-sampling is a reliable and feasible alternative to venepuncture for serological assessment in COVID-19.
format	article
author	Lottie Brown Rachel L. Byrne Alice Fraser Sophie I. Owen Ana I. Cubas-Atienzar Christopher T. Williams Grant A. Kay Luis E. Cuevas Joseph R. A. Fitchett Tom Fletcher Gala Garrod Konstantina Kontogianni Sanjeev Krishna Stefanie Menzies Tim Planche Chris Sainter Henry M. Staines Lance Turtle Emily R. Adams
author_facet	Lottie Brown Rachel L. Byrne Alice Fraser Sophie I. Owen Ana I. Cubas-Atienzar Christopher T. Williams Grant A. Kay Luis E. Cuevas Joseph R. A. Fitchett Tom Fletcher Gala Garrod Konstantina Kontogianni Sanjeev Krishna Stefanie Menzies Tim Planche Chris Sainter Henry M. Staines Lance Turtle Emily R. Adams
author_sort	Lottie Brown
title	Self-sampling of capillary blood for SARS-CoV-2 serology
title_short	Self-sampling of capillary blood for SARS-CoV-2 serology
title_full	Self-sampling of capillary blood for SARS-CoV-2 serology
title_fullStr	Self-sampling of capillary blood for SARS-CoV-2 serology
title_full_unstemmed	Self-sampling of capillary blood for SARS-CoV-2 serology
title_sort	self-sampling of capillary blood for sars-cov-2 serology
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9aa2f5d1ea794a83af7a2a1c7036d7bf
work_keys_str_mv	AT lottiebrown selfsamplingofcapillarybloodforsarscov2serology AT rachellbyrne selfsamplingofcapillarybloodforsarscov2serology AT alicefraser selfsamplingofcapillarybloodforsarscov2serology AT sophieiowen selfsamplingofcapillarybloodforsarscov2serology AT anaicubasatienzar selfsamplingofcapillarybloodforsarscov2serology AT christophertwilliams selfsamplingofcapillarybloodforsarscov2serology AT grantakay selfsamplingofcapillarybloodforsarscov2serology AT luisecuevas selfsamplingofcapillarybloodforsarscov2serology AT josephrafitchett selfsamplingofcapillarybloodforsarscov2serology AT tomfletcher selfsamplingofcapillarybloodforsarscov2serology AT galagarrod selfsamplingofcapillarybloodforsarscov2serology AT konstantinakontogianni selfsamplingofcapillarybloodforsarscov2serology AT sanjeevkrishna selfsamplingofcapillarybloodforsarscov2serology AT stefaniemenzies selfsamplingofcapillarybloodforsarscov2serology AT timplanche selfsamplingofcapillarybloodforsarscov2serology AT chrissainter selfsamplingofcapillarybloodforsarscov2serology AT henrymstaines selfsamplingofcapillarybloodforsarscov2serology AT lanceturtle selfsamplingofcapillarybloodforsarscov2serology AT emilyradams selfsamplingofcapillarybloodforsarscov2serology
_version_	1718390966951346176

Self-sampling of capillary blood for SARS-CoV-2 serology

Ejemplares similares