A subset of cerebrovascular pericytes originates from mature macrophages in the very early phase of vascular development in CNS
Abstract Pericytes are believed to originate from either mesenchymal or neural crest cells. It has recently been reported that pericytes play important roles in the central nervous system (CNS) by regulating blood-brain barrier homeostasis and blood flow at the capillary level. However, the origin o...
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2017
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oai:doaj.org-article:9aa3cc86afa343a8b1e09b837010f4832021-12-02T12:30:51ZA subset of cerebrovascular pericytes originates from mature macrophages in the very early phase of vascular development in CNS10.1038/s41598-017-03994-12045-2322https://doaj.org/article/9aa3cc86afa343a8b1e09b837010f4832017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03994-1https://doaj.org/toc/2045-2322Abstract Pericytes are believed to originate from either mesenchymal or neural crest cells. It has recently been reported that pericytes play important roles in the central nervous system (CNS) by regulating blood-brain barrier homeostasis and blood flow at the capillary level. However, the origin of CNS microvascular pericytes and the mechanism of their recruitment remain unknown. Here, we show a new source of cerebrovascular pericytes during neurogenesis. In the CNS of embryonic day 10.5 mouse embryos, CD31+F4/80+ hematopoietic lineage cells were observed in the avascular region around the dorsal midline of the developing midbrain. These cells expressed additional macrophage markers such as CD206 and CD11b. Moreover, the CD31+F4/80+ cells phagocytosed apoptotic cells as functionally matured macrophages, adhered to the newly formed subventricular vascular plexus, and then divided into daughter cells. Eventually, these CD31+F4/80+ cells transdifferentiated into NG2/PDGFRβ/desmin-expressing cerebrovascular pericytes, enwrapping and associating with vascular endothelial cells. These data indicate that a subset of cerebrovascular pericytes derive from mature macrophages in the very early phase of CNS vascular development, which in turn are recruited from sites of embryonic hematopoiesis such as the yolk sac by way of blood flow.Seiji YamamotoMasashi MuramatsuErika AzumaMasashi IkutaniYoshinori NagaiHiroshi SagaraBon-Nyeo KooSatomi KitaErin O’DonnellTsuyoshi OsawaHiroyuki TakahashiKen-ichi TakanoMitsuko DohmotoMichiya SugimoriIsao UsuiYasuhide WatanabeNoboru HatakeyamaTakahiro IwamotoIssei KomuroKiyoshi TakatsuKazuyuki TobeShumpei NiidaNaoyuki MatsudaMasabumi ShibuyaMasakiyo SasaharaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017) |
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Medicine R Science Q Seiji Yamamoto Masashi Muramatsu Erika Azuma Masashi Ikutani Yoshinori Nagai Hiroshi Sagara Bon-Nyeo Koo Satomi Kita Erin O’Donnell Tsuyoshi Osawa Hiroyuki Takahashi Ken-ichi Takano Mitsuko Dohmoto Michiya Sugimori Isao Usui Yasuhide Watanabe Noboru Hatakeyama Takahiro Iwamoto Issei Komuro Kiyoshi Takatsu Kazuyuki Tobe Shumpei Niida Naoyuki Matsuda Masabumi Shibuya Masakiyo Sasahara A subset of cerebrovascular pericytes originates from mature macrophages in the very early phase of vascular development in CNS |
| description |
Abstract Pericytes are believed to originate from either mesenchymal or neural crest cells. It has recently been reported that pericytes play important roles in the central nervous system (CNS) by regulating blood-brain barrier homeostasis and blood flow at the capillary level. However, the origin of CNS microvascular pericytes and the mechanism of their recruitment remain unknown. Here, we show a new source of cerebrovascular pericytes during neurogenesis. In the CNS of embryonic day 10.5 mouse embryos, CD31+F4/80+ hematopoietic lineage cells were observed in the avascular region around the dorsal midline of the developing midbrain. These cells expressed additional macrophage markers such as CD206 and CD11b. Moreover, the CD31+F4/80+ cells phagocytosed apoptotic cells as functionally matured macrophages, adhered to the newly formed subventricular vascular plexus, and then divided into daughter cells. Eventually, these CD31+F4/80+ cells transdifferentiated into NG2/PDGFRβ/desmin-expressing cerebrovascular pericytes, enwrapping and associating with vascular endothelial cells. These data indicate that a subset of cerebrovascular pericytes derive from mature macrophages in the very early phase of CNS vascular development, which in turn are recruited from sites of embryonic hematopoiesis such as the yolk sac by way of blood flow. |
| format |
article |
| author |
Seiji Yamamoto Masashi Muramatsu Erika Azuma Masashi Ikutani Yoshinori Nagai Hiroshi Sagara Bon-Nyeo Koo Satomi Kita Erin O’Donnell Tsuyoshi Osawa Hiroyuki Takahashi Ken-ichi Takano Mitsuko Dohmoto Michiya Sugimori Isao Usui Yasuhide Watanabe Noboru Hatakeyama Takahiro Iwamoto Issei Komuro Kiyoshi Takatsu Kazuyuki Tobe Shumpei Niida Naoyuki Matsuda Masabumi Shibuya Masakiyo Sasahara |
| author_facet |
Seiji Yamamoto Masashi Muramatsu Erika Azuma Masashi Ikutani Yoshinori Nagai Hiroshi Sagara Bon-Nyeo Koo Satomi Kita Erin O’Donnell Tsuyoshi Osawa Hiroyuki Takahashi Ken-ichi Takano Mitsuko Dohmoto Michiya Sugimori Isao Usui Yasuhide Watanabe Noboru Hatakeyama Takahiro Iwamoto Issei Komuro Kiyoshi Takatsu Kazuyuki Tobe Shumpei Niida Naoyuki Matsuda Masabumi Shibuya Masakiyo Sasahara |
| author_sort |
Seiji Yamamoto |
| title |
A subset of cerebrovascular pericytes originates from mature macrophages in the very early phase of vascular development in CNS |
| title_short |
A subset of cerebrovascular pericytes originates from mature macrophages in the very early phase of vascular development in CNS |
| title_full |
A subset of cerebrovascular pericytes originates from mature macrophages in the very early phase of vascular development in CNS |
| title_fullStr |
A subset of cerebrovascular pericytes originates from mature macrophages in the very early phase of vascular development in CNS |
| title_full_unstemmed |
A subset of cerebrovascular pericytes originates from mature macrophages in the very early phase of vascular development in CNS |
| title_sort |
subset of cerebrovascular pericytes originates from mature macrophages in the very early phase of vascular development in cns |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/9aa3cc86afa343a8b1e09b837010f483 |
| work_keys_str_mv |
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