Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes

Abstract Staphylococcus epidermidis (S. epidermidis) ATCC 12228 was incubated with 2% polyethylene glycol (PEG)-8 Laurate to yield electricity which was measured by a voltage difference between electrodes. Production of electron was validated by a Ferrozine assay. The anti-Cutibacterium acnes (C. ac...

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Autores principales:	Shinta Marito, Sunita Keshari, Supitchaya Traisaeng, Do Thi Tra My, Arun Balasubramaniam, Prakoso Adi, Ming-Fa Hsieh, Deron Raymond Herr, Chun-Ming Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9aa6e03f045a43ebb0e20def25d9cd4b
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spelling	oai:doaj.org-article:9aa6e03f045a43ebb0e20def25d9cd4b2021-12-02T17:38:26ZElectricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes10.1038/s41598-021-91398-72045-2322https://doaj.org/article/9aa6e03f045a43ebb0e20def25d9cd4b2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91398-7https://doaj.org/toc/2045-2322Abstract Staphylococcus epidermidis (S. epidermidis) ATCC 12228 was incubated with 2% polyethylene glycol (PEG)-8 Laurate to yield electricity which was measured by a voltage difference between electrodes. Production of electron was validated by a Ferrozine assay. The anti-Cutibacterium acnes (C. acnes) activity of electrogenic S. epidermidis was assessed in vitro and in vivo. The voltage change (~ 4.4 mV) reached a peak 60 min after pipetting S. epidermidis plus 2% PEG-8 Laurate onto anodes. The electricity produced by S. epidermidis caused significant growth attenuation and cell lysis of C. acnes. Intradermal injection of C. acnes and S. epidermidis plus PEG-8 Laurate into the mouse ear considerably suppressed the growth of C. acnes. This suppressive effect was noticeably reversed when cyclophilin A of S. epidermidis was inhibited, indicating the essential role of cyclophilin A in electricity production of S. epidermidis against C. acnes. In summary, we demonstrate for the first time that skin S. epidermidis, in the presence of PEG-8 Laurate, can mediate cyclophilin A to elicit an electrical current that has anti-C. acnes effects. Electricity generated by S. epidermidis may confer immediate innate immunity in acne lesions to rein in the overgrowth of C. acnes at the onset of acne vulgaris.Shinta MaritoSunita KeshariSupitchaya TraisaengDo Thi Tra MyArun BalasubramaniamPrakoso AdiMing-Fa HsiehDeron Raymond HerrChun-Ming HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shinta Marito Sunita Keshari Supitchaya Traisaeng Do Thi Tra My Arun Balasubramaniam Prakoso Adi Ming-Fa Hsieh Deron Raymond Herr Chun-Ming Huang Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes
description	Abstract Staphylococcus epidermidis (S. epidermidis) ATCC 12228 was incubated with 2% polyethylene glycol (PEG)-8 Laurate to yield electricity which was measured by a voltage difference between electrodes. Production of electron was validated by a Ferrozine assay. The anti-Cutibacterium acnes (C. acnes) activity of electrogenic S. epidermidis was assessed in vitro and in vivo. The voltage change (~ 4.4 mV) reached a peak 60 min after pipetting S. epidermidis plus 2% PEG-8 Laurate onto anodes. The electricity produced by S. epidermidis caused significant growth attenuation and cell lysis of C. acnes. Intradermal injection of C. acnes and S. epidermidis plus PEG-8 Laurate into the mouse ear considerably suppressed the growth of C. acnes. This suppressive effect was noticeably reversed when cyclophilin A of S. epidermidis was inhibited, indicating the essential role of cyclophilin A in electricity production of S. epidermidis against C. acnes. In summary, we demonstrate for the first time that skin S. epidermidis, in the presence of PEG-8 Laurate, can mediate cyclophilin A to elicit an electrical current that has anti-C. acnes effects. Electricity generated by S. epidermidis may confer immediate innate immunity in acne lesions to rein in the overgrowth of C. acnes at the onset of acne vulgaris.
format	article
author	Shinta Marito Sunita Keshari Supitchaya Traisaeng Do Thi Tra My Arun Balasubramaniam Prakoso Adi Ming-Fa Hsieh Deron Raymond Herr Chun-Ming Huang
author_facet	Shinta Marito Sunita Keshari Supitchaya Traisaeng Do Thi Tra My Arun Balasubramaniam Prakoso Adi Ming-Fa Hsieh Deron Raymond Herr Chun-Ming Huang
author_sort	Shinta Marito
title	Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes
title_short	Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes
title_full	Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes
title_fullStr	Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes
title_full_unstemmed	Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes
title_sort	electricity-producing staphylococcus epidermidis counteracts cutibacterium acnes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9aa6e03f045a43ebb0e20def25d9cd4b
work_keys_str_mv	AT shintamarito electricityproducingstaphylococcusepidermidiscounteractscutibacteriumacnes AT sunitakeshari electricityproducingstaphylococcusepidermidiscounteractscutibacteriumacnes AT supitchayatraisaeng electricityproducingstaphylococcusepidermidiscounteractscutibacteriumacnes AT dothitramy electricityproducingstaphylococcusepidermidiscounteractscutibacteriumacnes AT arunbalasubramaniam electricityproducingstaphylococcusepidermidiscounteractscutibacteriumacnes AT prakosoadi electricityproducingstaphylococcusepidermidiscounteractscutibacteriumacnes AT mingfahsieh electricityproducingstaphylococcusepidermidiscounteractscutibacteriumacnes AT deronraymondherr electricityproducingstaphylococcusepidermidiscounteractscutibacteriumacnes AT chunminghuang electricityproducingstaphylococcusepidermidiscounteractscutibacteriumacnes
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Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes

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