Secretory laccase 1 in Bemisia tabaci MED is involved in whitefly-plant interaction

Abstract The whitefly Bemisia tabaci is a phloem-feeding pest that lives predominantly on herbaceous species and causes serious damage to hosts. Whitefly saliva is thought to contain proteins that modulate plant defences and facilitate feeding. A predicted secreted protein, laccase 1 (LAC1), was fou...

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Autores principales:	Chun-Hong Yang, Jian-Yang Guo, Dong Chu, Tian-Bo Ding, Ke-Ke Wei, Deng-Fa Cheng, Fang-Hao Wan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9aa70906be0041cb92deec05f9262e99
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spelling	oai:doaj.org-article:9aa70906be0041cb92deec05f9262e992021-12-02T15:05:09ZSecretory laccase 1 in Bemisia tabaci MED is involved in whitefly-plant interaction10.1038/s41598-017-03765-y2045-2322https://doaj.org/article/9aa70906be0041cb92deec05f9262e992017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03765-yhttps://doaj.org/toc/2045-2322Abstract The whitefly Bemisia tabaci is a phloem-feeding pest that lives predominantly on herbaceous species and causes serious damage to hosts. Whitefly saliva is thought to contain proteins that modulate plant defences and facilitate feeding. A predicted secreted protein, laccase 1 (LAC1), was found in the salivary gland transcriptome of B. tabaci and might be existed in the watery saliva of B. tabaci. As LAC1 has a potential role in detoxification of secondary plant compounds in insects, we speculated that it may participate in the insect’s response to plant defences. Here, we cloned the complete cDNA of LAC1 and found that (1) LAC1 was highly expressed in the salivary gland (SG) and midgut; (2) LAC1 transcript level in head (containing SG) was 2.1 times higher in plant-fed than in diet-fed whiteflies and 1.6 times higher in the head and 23.8 times higher in the midgut of whiteflies that fed on jasmonic acid (JA)-sprayed plants than on control plants; and (3) silencing LAC1 decreased the survival rate of plant-fed whiteflies but had a marginal effect on whiteflies raised on an artificial diet. These results indicate that LAC1 enables whiteflies to overcome the chemical defences of host plants and might act as an effector in saliva.Chun-Hong YangJian-Yang GuoDong ChuTian-Bo DingKe-Ke WeiDeng-Fa ChengFang-Hao WanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chun-Hong Yang Jian-Yang Guo Dong Chu Tian-Bo Ding Ke-Ke Wei Deng-Fa Cheng Fang-Hao Wan Secretory laccase 1 in Bemisia tabaci MED is involved in whitefly-plant interaction
description	Abstract The whitefly Bemisia tabaci is a phloem-feeding pest that lives predominantly on herbaceous species and causes serious damage to hosts. Whitefly saliva is thought to contain proteins that modulate plant defences and facilitate feeding. A predicted secreted protein, laccase 1 (LAC1), was found in the salivary gland transcriptome of B. tabaci and might be existed in the watery saliva of B. tabaci. As LAC1 has a potential role in detoxification of secondary plant compounds in insects, we speculated that it may participate in the insect’s response to plant defences. Here, we cloned the complete cDNA of LAC1 and found that (1) LAC1 was highly expressed in the salivary gland (SG) and midgut; (2) LAC1 transcript level in head (containing SG) was 2.1 times higher in plant-fed than in diet-fed whiteflies and 1.6 times higher in the head and 23.8 times higher in the midgut of whiteflies that fed on jasmonic acid (JA)-sprayed plants than on control plants; and (3) silencing LAC1 decreased the survival rate of plant-fed whiteflies but had a marginal effect on whiteflies raised on an artificial diet. These results indicate that LAC1 enables whiteflies to overcome the chemical defences of host plants and might act as an effector in saliva.
format	article
author	Chun-Hong Yang Jian-Yang Guo Dong Chu Tian-Bo Ding Ke-Ke Wei Deng-Fa Cheng Fang-Hao Wan
author_facet	Chun-Hong Yang Jian-Yang Guo Dong Chu Tian-Bo Ding Ke-Ke Wei Deng-Fa Cheng Fang-Hao Wan
author_sort	Chun-Hong Yang
title	Secretory laccase 1 in Bemisia tabaci MED is involved in whitefly-plant interaction
title_short	Secretory laccase 1 in Bemisia tabaci MED is involved in whitefly-plant interaction
title_full	Secretory laccase 1 in Bemisia tabaci MED is involved in whitefly-plant interaction
title_fullStr	Secretory laccase 1 in Bemisia tabaci MED is involved in whitefly-plant interaction
title_full_unstemmed	Secretory laccase 1 in Bemisia tabaci MED is involved in whitefly-plant interaction
title_sort	secretory laccase 1 in bemisia tabaci med is involved in whitefly-plant interaction
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9aa70906be0041cb92deec05f9262e99
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Secretory laccase 1 in Bemisia tabaci MED is involved in whitefly-plant interaction

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