Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study

Chenwen Li,1,* Ruoqiu Fu,1,* Caiping Yu,1 Zhuoheng Li,1 Haiyan Guan,1 Daqiang Hu,1 Dehua Zhao,2 Laichun Lu1 1Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 2College of Pharmacy, Chongqing Medical University, Chongqing, People's Repu...

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Autores principales:	Li CW, Fu RQ, Yu CP, Li ZH, Guan HY, Hu DQ, Zhao DH, Lu LC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9aa76910c44242c69d40f83c7cf4f82e
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spelling	oai:doaj.org-article:9aa76910c44242c69d40f83c7cf4f82e2021-12-02T09:08:47ZSilver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study1176-91141178-2013https://doaj.org/article/9aa76910c44242c69d40f83c7cf4f82e2013-11-01T00:00:00Zhttp://www.dovepress.com/silver-nanoparticlechitosan-oligosaccharidepolyvinyl-alcohol-nanofiber-a14854https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Chenwen Li,1,* Ruoqiu Fu,1,* Caiping Yu,1 Zhuoheng Li,1 Haiyan Guan,1 Daqiang Hu,1 Dehua Zhao,2 Laichun Lu1 1Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 2College of Pharmacy, Chongqing Medical University, Chongqing, People's Republic of China *These authors contributed equally to this work Abstract: In this study, a mixture of poly(vinyl alcohol) (PVA) and chitosan oligosaccharides (COS) was electrospun with silver nanoparticles (AgNPs) to produce fibrous mats for use in wound healing. The AgNPs were reduced by COS prior to electrospinning or Ag+ was reduced via ultraviolet irradiation in nanofibers. The morphologies of the PVA/COS/AgNO3 and PVA/COS-AgNP nanofibers were analyzed by scanning electron microscopy. Formation of the AgNPs was investigated by field emission transmission electron microscopy, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. We also evaluated the biocompatibility of the nanofibers, particularly their cytotoxicity to human skin fibroblasts and potential to cause primary skin irritation. The in vitro antibacterial activity and in vivo wound healing capacity of the nanofibers were also investigated. The nanofibers had a smooth surface with an average diameter of 130–192 nm. The diameters of the AgNPs were in the range of 15–22 nm. The nanofibers significantly inhibited growth of Escherichia coli and Staphylococcus aureus bacteria. PVA/COS-AgNP nanofibers accelerated the rate of wound healing over that of the control (gauze). The results of our in vitro and in vivo animal experiments suggest that PVA/COS-AgNP nanofibers should be of greater interest than PVA/COS/AgNO3 nanofibers for clinical use as a bioactive wound dressing. Keywords: preclinical study, wound dressing, electrospinning, silver nanoparticles, poly(vinyl alcohol), chitosan oligosaccharidesLi CWFu RQYu CPLi ZHGuan HYHu DQZhao DHLu LCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 4131-4145 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Li CW Fu RQ Yu CP Li ZH Guan HY Hu DQ Zhao DH Lu LC Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study
description	Chenwen Li,1,* Ruoqiu Fu,1,* Caiping Yu,1 Zhuoheng Li,1 Haiyan Guan,1 Daqiang Hu,1 Dehua Zhao,2 Laichun Lu1 1Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 2College of Pharmacy, Chongqing Medical University, Chongqing, People's Republic of China *These authors contributed equally to this work Abstract: In this study, a mixture of poly(vinyl alcohol) (PVA) and chitosan oligosaccharides (COS) was electrospun with silver nanoparticles (AgNPs) to produce fibrous mats for use in wound healing. The AgNPs were reduced by COS prior to electrospinning or Ag+ was reduced via ultraviolet irradiation in nanofibers. The morphologies of the PVA/COS/AgNO3 and PVA/COS-AgNP nanofibers were analyzed by scanning electron microscopy. Formation of the AgNPs was investigated by field emission transmission electron microscopy, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. We also evaluated the biocompatibility of the nanofibers, particularly their cytotoxicity to human skin fibroblasts and potential to cause primary skin irritation. The in vitro antibacterial activity and in vivo wound healing capacity of the nanofibers were also investigated. The nanofibers had a smooth surface with an average diameter of 130–192 nm. The diameters of the AgNPs were in the range of 15–22 nm. The nanofibers significantly inhibited growth of Escherichia coli and Staphylococcus aureus bacteria. PVA/COS-AgNP nanofibers accelerated the rate of wound healing over that of the control (gauze). The results of our in vitro and in vivo animal experiments suggest that PVA/COS-AgNP nanofibers should be of greater interest than PVA/COS/AgNO3 nanofibers for clinical use as a bioactive wound dressing. Keywords: preclinical study, wound dressing, electrospinning, silver nanoparticles, poly(vinyl alcohol), chitosan oligosaccharides
format	article
author	Li CW Fu RQ Yu CP Li ZH Guan HY Hu DQ Zhao DH Lu LC
author_facet	Li CW Fu RQ Yu CP Li ZH Guan HY Hu DQ Zhao DH Lu LC
author_sort	Li CW
title	Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study
title_short	Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study
title_full	Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study
title_fullStr	Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study
title_full_unstemmed	Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study
title_sort	silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/9aa76910c44242c69d40f83c7cf4f82e
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Silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) nanofibers as wound dressings: a preclinical study

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