Serotyping of Toxoplasma gondii in cats (Felis domesticus) reveals predominance of type II infections in Germany.

<h4>Background</h4>Cats are definitive hosts of Toxoplasma gondii and play an essential role in the epidemiology of this parasite. The study aims at clarifying whether cats are able to develop specific antibodies against different clonal types of T. gondii and to determine by serotyping...

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Autores principales:	Pavlo Maksimov, Johannes Zerweck, Jitender P Dubey, Nikola Pantchev, Caroline F Frey, Aline Maksimov, Ulf Reimer, Mike Schutkowski, Morteza Hosseininejad, Mario Ziller, Franz J Conraths, Gereon Schares
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9aa952d432b64cb9a22cff440319bf22
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spelling	oai:doaj.org-article:9aa952d432b64cb9a22cff440319bf222021-11-18T08:47:51ZSerotyping of Toxoplasma gondii in cats (Felis domesticus) reveals predominance of type II infections in Germany.1932-620310.1371/journal.pone.0080213https://doaj.org/article/9aa952d432b64cb9a22cff440319bf222013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24244652/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Cats are definitive hosts of Toxoplasma gondii and play an essential role in the epidemiology of this parasite. The study aims at clarifying whether cats are able to develop specific antibodies against different clonal types of T. gondii and to determine by serotyping the T. gondii clonal types prevailing in cats as intermediate hosts in Germany.<h4>Methodology</h4>To establish a peptide-microarray serotyping test, we identified 24 suitable peptides using serological T. gondii positive (n=21) and negative cat sera (n=52). To determine the clonal type-specific antibody response of cats in Germany, 86 field sera from T. gondii seropositive naturally infected cats were tested. In addition, we analyzed the antibody response in cats experimentally infected with non-canonical T. gondii types (n=7).<h4>Findings</h4>Positive cat reference sera reacted predominantly with peptides harbouring amino acid sequences specific for the clonal T. gondii type the cats were infected with. When the array was applied to field sera from Germany, 98.8% (85/86) of naturally-infected cats recognized similar peptide patterns as T. gondii type II reference sera and showed the strongest reaction intensities with clonal type II-specific peptides. In addition, naturally infected cats recognized type II-specific peptides significantly more frequently than peptides of other type-specificities. Cats infected with non-canonical types showed the strongest reactivity with peptides presenting amino-acid sequences specific for both, type I and type III.<h4>Conclusions</h4>Cats are able to mount a clonal type-specific antibody response against T. gondii. Serotyping revealed for most seropositive field sera patterns resembling those observed after clonal type II-T. gondii infection. This finding is in accord with our previous results on the occurrence of T. gondii clonal types in oocysts shed by cats in Germany.Pavlo MaksimovJohannes ZerweckJitender P DubeyNikola PantchevCaroline F FreyAline MaksimovUlf ReimerMike SchutkowskiMorteza HosseininejadMario ZillerFranz J ConrathsGereon ScharesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80213 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pavlo Maksimov Johannes Zerweck Jitender P Dubey Nikola Pantchev Caroline F Frey Aline Maksimov Ulf Reimer Mike Schutkowski Morteza Hosseininejad Mario Ziller Franz J Conraths Gereon Schares Serotyping of Toxoplasma gondii in cats (Felis domesticus) reveals predominance of type II infections in Germany.
description	<h4>Background</h4>Cats are definitive hosts of Toxoplasma gondii and play an essential role in the epidemiology of this parasite. The study aims at clarifying whether cats are able to develop specific antibodies against different clonal types of T. gondii and to determine by serotyping the T. gondii clonal types prevailing in cats as intermediate hosts in Germany.<h4>Methodology</h4>To establish a peptide-microarray serotyping test, we identified 24 suitable peptides using serological T. gondii positive (n=21) and negative cat sera (n=52). To determine the clonal type-specific antibody response of cats in Germany, 86 field sera from T. gondii seropositive naturally infected cats were tested. In addition, we analyzed the antibody response in cats experimentally infected with non-canonical T. gondii types (n=7).<h4>Findings</h4>Positive cat reference sera reacted predominantly with peptides harbouring amino acid sequences specific for the clonal T. gondii type the cats were infected with. When the array was applied to field sera from Germany, 98.8% (85/86) of naturally-infected cats recognized similar peptide patterns as T. gondii type II reference sera and showed the strongest reaction intensities with clonal type II-specific peptides. In addition, naturally infected cats recognized type II-specific peptides significantly more frequently than peptides of other type-specificities. Cats infected with non-canonical types showed the strongest reactivity with peptides presenting amino-acid sequences specific for both, type I and type III.<h4>Conclusions</h4>Cats are able to mount a clonal type-specific antibody response against T. gondii. Serotyping revealed for most seropositive field sera patterns resembling those observed after clonal type II-T. gondii infection. This finding is in accord with our previous results on the occurrence of T. gondii clonal types in oocysts shed by cats in Germany.
format	article
author	Pavlo Maksimov Johannes Zerweck Jitender P Dubey Nikola Pantchev Caroline F Frey Aline Maksimov Ulf Reimer Mike Schutkowski Morteza Hosseininejad Mario Ziller Franz J Conraths Gereon Schares
author_facet	Pavlo Maksimov Johannes Zerweck Jitender P Dubey Nikola Pantchev Caroline F Frey Aline Maksimov Ulf Reimer Mike Schutkowski Morteza Hosseininejad Mario Ziller Franz J Conraths Gereon Schares
author_sort	Pavlo Maksimov
title	Serotyping of Toxoplasma gondii in cats (Felis domesticus) reveals predominance of type II infections in Germany.
title_short	Serotyping of Toxoplasma gondii in cats (Felis domesticus) reveals predominance of type II infections in Germany.
title_full	Serotyping of Toxoplasma gondii in cats (Felis domesticus) reveals predominance of type II infections in Germany.
title_fullStr	Serotyping of Toxoplasma gondii in cats (Felis domesticus) reveals predominance of type II infections in Germany.
title_full_unstemmed	Serotyping of Toxoplasma gondii in cats (Felis domesticus) reveals predominance of type II infections in Germany.
title_sort	serotyping of toxoplasma gondii in cats (felis domesticus) reveals predominance of type ii infections in germany.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/9aa952d432b64cb9a22cff440319bf22
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Serotyping of Toxoplasma gondii in cats (Felis domesticus) reveals predominance of type II infections in Germany.

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