Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension.

Vps13 family proteins are proposed to function in bulk lipid transfer between membranes, but little is known about their regulation. During sporulation of Saccharomyces cerevisiae, Vps13 localizes to the prospore membrane (PSM) via the Spo71-Spo73 adaptor complex. We previously reported that loss of...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Tsuyoshi S Nakamura, Yasuyuki Suda, Kenji Muneshige, Yuji Fujieda, Yuuya Okumura, Ichiro Inoue, Takayuki Tanaka, Tetsuo Takahashi, Hideki Nakanishi, Xiao-Dong Gao, Yasushi Okada, Aaron M Neiman, Hiroyuki Tachikawa
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2021
Materias:
Acceso en línea:https://doaj.org/article/9ab51db7f8e54813a90466d78f16b47b
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:9ab51db7f8e54813a90466d78f16b47b
record_format dspace
spelling oai:doaj.org-article:9ab51db7f8e54813a90466d78f16b47b2021-12-02T20:02:52ZSuppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension.1553-73901553-740410.1371/journal.pgen.1009727https://doaj.org/article/9ab51db7f8e54813a90466d78f16b47b2021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009727https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Vps13 family proteins are proposed to function in bulk lipid transfer between membranes, but little is known about their regulation. During sporulation of Saccharomyces cerevisiae, Vps13 localizes to the prospore membrane (PSM) via the Spo71-Spo73 adaptor complex. We previously reported that loss of any of these proteins causes PSM extension and subsequent sporulation defects, yet their precise function remains unclear. Here, we performed a genetic screen and identified genes coding for a fragment of phosphatidylinositol (PI) 4-kinase catalytic subunit and PI 4-kinase noncatalytic subunit as multicopy suppressors of spo73Δ. Further genetic and cytological analyses revealed that lowering PI4P levels in the PSM rescues the spo73Δ defects. Furthermore, overexpression of VPS13 and lowering PI4P levels synergistically rescued the defect of a spo71Δ spo73Δ double mutant, suggesting that PI4P might regulate Vps13 function. In addition, we show that an N-terminal fragment of Vps13 has affinity for the endoplasmic reticulum (ER), and ER-plasma membrane (PM) tethers localize along the PSM in a manner dependent on Vps13 and the adaptor complex. These observations suggest that Vps13 and the adaptor complex recruit ER-PM tethers to ER-PSM contact sites. Our analysis revealed that involvement of a phosphoinositide, PI4P, in regulation of Vps13, and also suggest that distinct contact site proteins function cooperatively to promote de novo membrane formation.Tsuyoshi S NakamuraYasuyuki SudaKenji MuneshigeYuji FujiedaYuuya OkumuraIchiro InoueTakayuki TanakaTetsuo TakahashiHideki NakanishiXiao-Dong GaoYasushi OkadaAaron M NeimanHiroyuki TachikawaPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009727 (2021)
institution DOAJ
collection DOAJ
language EN
topic Genetics
QH426-470
spellingShingle Genetics
QH426-470
Tsuyoshi S Nakamura
Yasuyuki Suda
Kenji Muneshige
Yuji Fujieda
Yuuya Okumura
Ichiro Inoue
Takayuki Tanaka
Tetsuo Takahashi
Hideki Nakanishi
Xiao-Dong Gao
Yasushi Okada
Aaron M Neiman
Hiroyuki Tachikawa
Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension.
description Vps13 family proteins are proposed to function in bulk lipid transfer between membranes, but little is known about their regulation. During sporulation of Saccharomyces cerevisiae, Vps13 localizes to the prospore membrane (PSM) via the Spo71-Spo73 adaptor complex. We previously reported that loss of any of these proteins causes PSM extension and subsequent sporulation defects, yet their precise function remains unclear. Here, we performed a genetic screen and identified genes coding for a fragment of phosphatidylinositol (PI) 4-kinase catalytic subunit and PI 4-kinase noncatalytic subunit as multicopy suppressors of spo73Δ. Further genetic and cytological analyses revealed that lowering PI4P levels in the PSM rescues the spo73Δ defects. Furthermore, overexpression of VPS13 and lowering PI4P levels synergistically rescued the defect of a spo71Δ spo73Δ double mutant, suggesting that PI4P might regulate Vps13 function. In addition, we show that an N-terminal fragment of Vps13 has affinity for the endoplasmic reticulum (ER), and ER-plasma membrane (PM) tethers localize along the PSM in a manner dependent on Vps13 and the adaptor complex. These observations suggest that Vps13 and the adaptor complex recruit ER-PM tethers to ER-PSM contact sites. Our analysis revealed that involvement of a phosphoinositide, PI4P, in regulation of Vps13, and also suggest that distinct contact site proteins function cooperatively to promote de novo membrane formation.
format article
author Tsuyoshi S Nakamura
Yasuyuki Suda
Kenji Muneshige
Yuji Fujieda
Yuuya Okumura
Ichiro Inoue
Takayuki Tanaka
Tetsuo Takahashi
Hideki Nakanishi
Xiao-Dong Gao
Yasushi Okada
Aaron M Neiman
Hiroyuki Tachikawa
author_facet Tsuyoshi S Nakamura
Yasuyuki Suda
Kenji Muneshige
Yuji Fujieda
Yuuya Okumura
Ichiro Inoue
Takayuki Tanaka
Tetsuo Takahashi
Hideki Nakanishi
Xiao-Dong Gao
Yasushi Okada
Aaron M Neiman
Hiroyuki Tachikawa
author_sort Tsuyoshi S Nakamura
title Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension.
title_short Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension.
title_full Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension.
title_fullStr Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension.
title_full_unstemmed Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension.
title_sort suppression of vps13 adaptor protein mutants reveals a central role for pi4p in regulating prospore membrane extension.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/9ab51db7f8e54813a90466d78f16b47b
work_keys_str_mv AT tsuyoshisnakamura suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
AT yasuyukisuda suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
AT kenjimuneshige suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
AT yujifujieda suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
AT yuuyaokumura suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
AT ichiroinoue suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
AT takayukitanaka suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
AT tetsuotakahashi suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
AT hidekinakanishi suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
AT xiaodonggao suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
AT yasushiokada suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
AT aaronmneiman suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
AT hiroyukitachikawa suppressionofvps13adaptorproteinmutantsrevealsacentralroleforpi4pinregulatingprosporemembraneextension
_version_ 1718375674572439552