The interaction between flagellin and the glycosphingolipid Gb3 on host cells contributes to Bacillus cereus acute infection

Bacillus cereus is an opportunistic pathogen that can cause emetic or diarrheal foodborne illness. Previous studies have identified multiple pathogenic B. cereus strains and characterized a variety of virulence factors. Here, we demonstrate that the virulence and lethality of B. cereus for mammalian...

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Autores principales:	Song Gao, Chengpei Ni, Wenhua Huang, Huaijie Hao, Hua Jiang, Qingyu Lv, Yuling Zheng, Peng Liu, Decong Kong, Yongqiang Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	bacillus cereus gb3 flagellin adhesion virulence factor Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/9ab6fd4411404e929a08b6e675f3c490
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spelling	oai:doaj.org-article:9ab6fd4411404e929a08b6e675f3c4902021-11-17T14:21:58ZThe interaction between flagellin and the glycosphingolipid Gb3 on host cells contributes to Bacillus cereus acute infection2150-55942150-560810.1080/21505594.2020.1773077https://doaj.org/article/9ab6fd4411404e929a08b6e675f3c4902020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1773077https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Bacillus cereus is an opportunistic pathogen that can cause emetic or diarrheal foodborne illness. Previous studies have identified multiple pathogenic B. cereus strains and characterized a variety of virulence factors. Here, we demonstrate that the virulence and lethality of B. cereus for mammalian cells and host animals involve the interaction of B. cereus flagellin proteins and the host-cell-surface-localized glycosphingolipid Gb3 (CD77, Galα1-4Galβ1-4Glcβ1-Cer). We initially found that B. cereus infection was less lethal for Gb3-deficiencient A4galt−/- mice than for wild-type mice. Subsequent experiments established that some factor other than secreted toxins must account of the observed differential lethality: Gb3-deficiencient A4galt−/- mice were equally susceptible to secreted-virulence-factor-mediated death as WT mice, and we observed no differences in the bacterial loads of spleens or livers of mice treated with B. cereus strain vs. mice infected with a mutant variant of incapable of producing many secreted toxins. A screen for host-interacting B. cereus cell wall components identified the well-known flagellin protein, and both flagellin knockout strain assays and Gb3 inhibitor studies confirmed that flagellin does interact with Gb3 in a manner that affects B. cereus infection of host cells. Finally, we show that treatment with polyclonal antibody against flagellin can protect mice against B. cereus infection. Thus, beyond demonstrating a previously unappreciated interaction between a bacterial motor protein and a mammalian cell wall glycosphingolipid, our study will provide useful information for the development of therapies to treat infection of B. cereus.Song GaoChengpei NiWenhua HuangHuaijie HaoHua JiangQingyu LvYuling ZhengPeng LiuDecong KongYongqiang JiangTaylor & Francis Grouparticlebacillus cereusgb3flagellinadhesionvirulence factorInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 769-780 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacillus cereus gb3 flagellin adhesion virulence factor Infectious and parasitic diseases RC109-216
spellingShingle	bacillus cereus gb3 flagellin adhesion virulence factor Infectious and parasitic diseases RC109-216 Song Gao Chengpei Ni Wenhua Huang Huaijie Hao Hua Jiang Qingyu Lv Yuling Zheng Peng Liu Decong Kong Yongqiang Jiang The interaction between flagellin and the glycosphingolipid Gb3 on host cells contributes to Bacillus cereus acute infection
description	Bacillus cereus is an opportunistic pathogen that can cause emetic or diarrheal foodborne illness. Previous studies have identified multiple pathogenic B. cereus strains and characterized a variety of virulence factors. Here, we demonstrate that the virulence and lethality of B. cereus for mammalian cells and host animals involve the interaction of B. cereus flagellin proteins and the host-cell-surface-localized glycosphingolipid Gb3 (CD77, Galα1-4Galβ1-4Glcβ1-Cer). We initially found that B. cereus infection was less lethal for Gb3-deficiencient A4galt−/- mice than for wild-type mice. Subsequent experiments established that some factor other than secreted toxins must account of the observed differential lethality: Gb3-deficiencient A4galt−/- mice were equally susceptible to secreted-virulence-factor-mediated death as WT mice, and we observed no differences in the bacterial loads of spleens or livers of mice treated with B. cereus strain vs. mice infected with a mutant variant of incapable of producing many secreted toxins. A screen for host-interacting B. cereus cell wall components identified the well-known flagellin protein, and both flagellin knockout strain assays and Gb3 inhibitor studies confirmed that flagellin does interact with Gb3 in a manner that affects B. cereus infection of host cells. Finally, we show that treatment with polyclonal antibody against flagellin can protect mice against B. cereus infection. Thus, beyond demonstrating a previously unappreciated interaction between a bacterial motor protein and a mammalian cell wall glycosphingolipid, our study will provide useful information for the development of therapies to treat infection of B. cereus.
format	article
author	Song Gao Chengpei Ni Wenhua Huang Huaijie Hao Hua Jiang Qingyu Lv Yuling Zheng Peng Liu Decong Kong Yongqiang Jiang
author_facet	Song Gao Chengpei Ni Wenhua Huang Huaijie Hao Hua Jiang Qingyu Lv Yuling Zheng Peng Liu Decong Kong Yongqiang Jiang
author_sort	Song Gao
title	The interaction between flagellin and the glycosphingolipid Gb3 on host cells contributes to Bacillus cereus acute infection
title_short	The interaction between flagellin and the glycosphingolipid Gb3 on host cells contributes to Bacillus cereus acute infection
title_full	The interaction between flagellin and the glycosphingolipid Gb3 on host cells contributes to Bacillus cereus acute infection
title_fullStr	The interaction between flagellin and the glycosphingolipid Gb3 on host cells contributes to Bacillus cereus acute infection
title_full_unstemmed	The interaction between flagellin and the glycosphingolipid Gb3 on host cells contributes to Bacillus cereus acute infection
title_sort	interaction between flagellin and the glycosphingolipid gb3 on host cells contributes to bacillus cereus acute infection
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/9ab6fd4411404e929a08b6e675f3c490
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The interaction between flagellin and the glycosphingolipid Gb3 on host cells contributes to Bacillus cereus acute infection

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