Neutrophil granulocyte functional status and expression of apoptosis markers in children with type 1 diabetes

In recent years, polymorphonuclear leukocytes have been proven to play an important role in the development of diabetes mellitus (DM). Neutrophil dysfunction contributes to pancreatic tissue damage as well as an increased susceptibility to infection in type 1 DM (T1DM) patients. Objective. The obje...

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Autores principales:	Lyudmila Yur'evna Barycheva, Natalya Eduardovna Erdni-Goryaeva, Galina Alekseevna Alexandrovich
Formato:	article
Lenguaje:	EN RU
Publicado:	Endocrinology Research Centre 2014
Materias:	diabetes mellitus neutrophils phagocytosis myeloperoxidase lysosomal cationic proteins oxidase activity apoptosis Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/9ac7b05d3da44ca592a86db045a645fd
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spelling	oai:doaj.org-article:9ac7b05d3da44ca592a86db045a645fd2021-11-14T09:00:19ZNeutrophil granulocyte functional status and expression of apoptosis markers in children with type 1 diabetes2072-03512072-037810.14341/DM2014377-82https://doaj.org/article/9ac7b05d3da44ca592a86db045a645fd2014-10-01T00:00:00Zhttps://www.dia-endojournals.ru/jour/article/view/6496https://doaj.org/toc/2072-0351https://doaj.org/toc/2072-0378In recent years, polymorphonuclear leukocytes have been proven to play an important role in the development of diabetes mellitus (DM). Neutrophil dysfunction contributes to pancreatic tissue damage as well as an increased susceptibility to infection in type 1 DM (T1DM) patients. Objective. The objective of this study was to investigate the functional activity of neutrophil granulocytes (NGs) in children with T1DM. Materials and Methods. This study involved 25 children aged 7?15 years. To evaluate programmed cell death, the number of NGs expressing apoptosis markers (CD95, CD95L and BCL2) was determined. The functional activity of NGs was determined in terms of phagocytosis and the levels of myeloperoxidase, lysosomal cationic proteins and active oxygen radicals. Results. A reduction in the bactericidal activity of NGs with deficiencies in phagocytosis, secretion of active oxygen radicals and functional reserve was found. An increase in the apoptotic potential of NGs was demonstrated, which was accompanied by an increase in CD95 expression and a decrease in BCL2 expression. An increase in the cytotoxic potential of neutrophils in the form of enhanced levels of myeloperoxidase and lysosomal cationic proteins was revealed. Conclusions. Therefore, an increase in the apoptotic potential of NGs associated with functional and metabolic changes may reflect the active involvement of NGs in the immunopathogenesis of T1DM.Lyudmila Yur'evna BarychevaNatalya Eduardovna Erdni-GoryaevaGalina Alekseevna AlexandrovichEndocrinology Research Centrearticlediabetes mellitusneutrophilsphagocytosismyeloperoxidaselysosomal cationic proteinsoxidase activityapoptosisNutritional diseases. Deficiency diseasesRC620-627ENRUСахарный диабет, Vol 17, Iss 3, Pp 77-82 (2014)
institution	DOAJ
collection	DOAJ
language	EN RU
topic	diabetes mellitus neutrophils phagocytosis myeloperoxidase lysosomal cationic proteins oxidase activity apoptosis Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	diabetes mellitus neutrophils phagocytosis myeloperoxidase lysosomal cationic proteins oxidase activity apoptosis Nutritional diseases. Deficiency diseases RC620-627 Lyudmila Yur'evna Barycheva Natalya Eduardovna Erdni-Goryaeva Galina Alekseevna Alexandrovich Neutrophil granulocyte functional status and expression of apoptosis markers in children with type 1 diabetes
description	In recent years, polymorphonuclear leukocytes have been proven to play an important role in the development of diabetes mellitus (DM). Neutrophil dysfunction contributes to pancreatic tissue damage as well as an increased susceptibility to infection in type 1 DM (T1DM) patients. Objective. The objective of this study was to investigate the functional activity of neutrophil granulocytes (NGs) in children with T1DM. Materials and Methods. This study involved 25 children aged 7?15 years. To evaluate programmed cell death, the number of NGs expressing apoptosis markers (CD95, CD95L and BCL2) was determined. The functional activity of NGs was determined in terms of phagocytosis and the levels of myeloperoxidase, lysosomal cationic proteins and active oxygen radicals. Results. A reduction in the bactericidal activity of NGs with deficiencies in phagocytosis, secretion of active oxygen radicals and functional reserve was found. An increase in the apoptotic potential of NGs was demonstrated, which was accompanied by an increase in CD95 expression and a decrease in BCL2 expression. An increase in the cytotoxic potential of neutrophils in the form of enhanced levels of myeloperoxidase and lysosomal cationic proteins was revealed. Conclusions. Therefore, an increase in the apoptotic potential of NGs associated with functional and metabolic changes may reflect the active involvement of NGs in the immunopathogenesis of T1DM.
format	article
author	Lyudmila Yur'evna Barycheva Natalya Eduardovna Erdni-Goryaeva Galina Alekseevna Alexandrovich
author_facet	Lyudmila Yur'evna Barycheva Natalya Eduardovna Erdni-Goryaeva Galina Alekseevna Alexandrovich
author_sort	Lyudmila Yur'evna Barycheva
title	Neutrophil granulocyte functional status and expression of apoptosis markers in children with type 1 diabetes
title_short	Neutrophil granulocyte functional status and expression of apoptosis markers in children with type 1 diabetes
title_full	Neutrophil granulocyte functional status and expression of apoptosis markers in children with type 1 diabetes
title_fullStr	Neutrophil granulocyte functional status and expression of apoptosis markers in children with type 1 diabetes
title_full_unstemmed	Neutrophil granulocyte functional status and expression of apoptosis markers in children with type 1 diabetes
title_sort	neutrophil granulocyte functional status and expression of apoptosis markers in children with type 1 diabetes
publisher	Endocrinology Research Centre
publishDate	2014
url	https://doaj.org/article/9ac7b05d3da44ca592a86db045a645fd
work_keys_str_mv	AT lyudmilayurevnabarycheva neutrophilgranulocytefunctionalstatusandexpressionofapoptosismarkersinchildrenwithtype1diabetes AT natalyaeduardovnaerdnigoryaeva neutrophilgranulocytefunctionalstatusandexpressionofapoptosismarkersinchildrenwithtype1diabetes AT galinaalekseevnaalexandrovich neutrophilgranulocytefunctionalstatusandexpressionofapoptosismarkersinchildrenwithtype1diabetes
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Neutrophil granulocyte functional status and expression of apoptosis markers in children with type 1 diabetes

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