Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene

During transcription, RNA polymerase II (RNAP2) is recruited to promoters and phosphorylated stepwise; so far, these steps have not been visualized in a single-copy gene in live cells. Here the authors use single-molecule microscopy to visualize endogenous phosphorylated RNAP2 and nascent mRNA synth...

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Autores principales:	Linda S. Forero-Quintero, William Raymond, Tetsuya Handa, Matthew N. Saxton, Tatsuya Morisaki, Hiroshi Kimura, Edouard Bertrand, Brian Munsky, Timothy J. Stasevich
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9ac9a177c745472c9f09664f8916d4d9
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spelling	oai:doaj.org-article:9ac9a177c745472c9f09664f8916d4d92021-12-02T16:53:16ZLive-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene10.1038/s41467-021-23417-02041-1723https://doaj.org/article/9ac9a177c745472c9f09664f8916d4d92021-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23417-0https://doaj.org/toc/2041-1723During transcription, RNA polymerase II (RNAP2) is recruited to promoters and phosphorylated stepwise; so far, these steps have not been visualized in a single-copy gene in live cells. Here the authors use single-molecule microscopy to visualize endogenous phosphorylated RNAP2 and nascent mRNA synthesis at a single locus in living cells.Linda S. Forero-QuinteroWilliam RaymondTetsuya HandaMatthew N. SaxtonTatsuya MorisakiHiroshi KimuraEdouard BertrandBrian MunskyTimothy J. StasevichNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Linda S. Forero-Quintero William Raymond Tetsuya Handa Matthew N. Saxton Tatsuya Morisaki Hiroshi Kimura Edouard Bertrand Brian Munsky Timothy J. Stasevich Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene
description	During transcription, RNA polymerase II (RNAP2) is recruited to promoters and phosphorylated stepwise; so far, these steps have not been visualized in a single-copy gene in live cells. Here the authors use single-molecule microscopy to visualize endogenous phosphorylated RNAP2 and nascent mRNA synthesis at a single locus in living cells.
format	article
author	Linda S. Forero-Quintero William Raymond Tetsuya Handa Matthew N. Saxton Tatsuya Morisaki Hiroshi Kimura Edouard Bertrand Brian Munsky Timothy J. Stasevich
author_facet	Linda S. Forero-Quintero William Raymond Tetsuya Handa Matthew N. Saxton Tatsuya Morisaki Hiroshi Kimura Edouard Bertrand Brian Munsky Timothy J. Stasevich
author_sort	Linda S. Forero-Quintero
title	Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene
title_short	Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene
title_full	Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene
title_fullStr	Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene
title_full_unstemmed	Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene
title_sort	live-cell imaging reveals the spatiotemporal organization of endogenous rna polymerase ii phosphorylation at a single gene
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9ac9a177c745472c9f09664f8916d4d9
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Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene

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