Establishment of a novel human lymphoblastic cell strain with the long arm of chromosome 11 aberration without MLL rearrangement

Abstract At present, all cell strains derived from acute lymphoblastic leukemia (ALL) patients with the long arm of chromosome 11 aberration are accompanied with mixed lineage leukemia (MLL) gene rearrangement. In this study, we established a permanent ALL cell strain CHH-1 with the long arm of chro...

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Autores principales:	Qian Wang, Lin Zhuang, Pei Li, Qiang Niu, Ping Zhu, Miao-Xia He, Hui Jiang, Chang-Cheng Liu, Min-Jun Wang, Li Chen, Hui Cheng, Yan Ma, Xiao-Xia Hu, Yi-Ping Hu, Xiao-Ping Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ad6fecdc8814178ac1bb2d97ac8277f
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spelling	oai:doaj.org-article:9ad6fecdc8814178ac1bb2d97ac8277f2021-12-02T15:05:49ZEstablishment of a novel human lymphoblastic cell strain with the long arm of chromosome 11 aberration without MLL rearrangement10.1038/s41598-017-00874-62045-2322https://doaj.org/article/9ad6fecdc8814178ac1bb2d97ac8277f2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00874-6https://doaj.org/toc/2045-2322Abstract At present, all cell strains derived from acute lymphoblastic leukemia (ALL) patients with the long arm of chromosome 11 aberration are accompanied with mixed lineage leukemia (MLL) gene rearrangement. In this study, we established a permanent ALL cell strain CHH-1 with the long arm of chromosome 11 aberration and without MLL rearrangement, hoping that it could be used for the research of ALL with such genetic abnormality. CHH-1 cell strain was certified through morphology, immunophenotype, genetics and immunoglobulin (Ig) gene rearrangement analysis. Cell characteristics including tumorigenic ability, semisolid colony forming ability, telomerase activity, autocrine and invasion were further detected. Cells were with an add(11)(q23) structural abnormality without MLL rearrangement, and were consistent with the genetic abnormality of the patient. In addition, these cells had features of tumor-forming ability, high colony forming capacity, unique cytokine autocrine mode, high telomerase activity, and high invasion ability. CHH-1 may prove to be a useful cell model for the research of human leukemia with genetic aberration in chromosome 11, and help explore the role of such genetic abnormality in the pathogenesis, progression and prognosis of ALL, and in developing new target drugs.Qian WangLin ZhuangPei LiQiang NiuPing ZhuMiao-Xia HeHui JiangChang-Cheng LiuMin-Jun WangLi ChenHui ChengYan MaXiao-Xia HuYi-Ping HuXiao-Ping XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qian Wang Lin Zhuang Pei Li Qiang Niu Ping Zhu Miao-Xia He Hui Jiang Chang-Cheng Liu Min-Jun Wang Li Chen Hui Cheng Yan Ma Xiao-Xia Hu Yi-Ping Hu Xiao-Ping Xu Establishment of a novel human lymphoblastic cell strain with the long arm of chromosome 11 aberration without MLL rearrangement
description	Abstract At present, all cell strains derived from acute lymphoblastic leukemia (ALL) patients with the long arm of chromosome 11 aberration are accompanied with mixed lineage leukemia (MLL) gene rearrangement. In this study, we established a permanent ALL cell strain CHH-1 with the long arm of chromosome 11 aberration and without MLL rearrangement, hoping that it could be used for the research of ALL with such genetic abnormality. CHH-1 cell strain was certified through morphology, immunophenotype, genetics and immunoglobulin (Ig) gene rearrangement analysis. Cell characteristics including tumorigenic ability, semisolid colony forming ability, telomerase activity, autocrine and invasion were further detected. Cells were with an add(11)(q23) structural abnormality without MLL rearrangement, and were consistent with the genetic abnormality of the patient. In addition, these cells had features of tumor-forming ability, high colony forming capacity, unique cytokine autocrine mode, high telomerase activity, and high invasion ability. CHH-1 may prove to be a useful cell model for the research of human leukemia with genetic aberration in chromosome 11, and help explore the role of such genetic abnormality in the pathogenesis, progression and prognosis of ALL, and in developing new target drugs.
format	article
author	Qian Wang Lin Zhuang Pei Li Qiang Niu Ping Zhu Miao-Xia He Hui Jiang Chang-Cheng Liu Min-Jun Wang Li Chen Hui Cheng Yan Ma Xiao-Xia Hu Yi-Ping Hu Xiao-Ping Xu
author_facet	Qian Wang Lin Zhuang Pei Li Qiang Niu Ping Zhu Miao-Xia He Hui Jiang Chang-Cheng Liu Min-Jun Wang Li Chen Hui Cheng Yan Ma Xiao-Xia Hu Yi-Ping Hu Xiao-Ping Xu
author_sort	Qian Wang
title	Establishment of a novel human lymphoblastic cell strain with the long arm of chromosome 11 aberration without MLL rearrangement
title_short	Establishment of a novel human lymphoblastic cell strain with the long arm of chromosome 11 aberration without MLL rearrangement
title_full	Establishment of a novel human lymphoblastic cell strain with the long arm of chromosome 11 aberration without MLL rearrangement
title_fullStr	Establishment of a novel human lymphoblastic cell strain with the long arm of chromosome 11 aberration without MLL rearrangement
title_full_unstemmed	Establishment of a novel human lymphoblastic cell strain with the long arm of chromosome 11 aberration without MLL rearrangement
title_sort	establishment of a novel human lymphoblastic cell strain with the long arm of chromosome 11 aberration without mll rearrangement
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9ad6fecdc8814178ac1bb2d97ac8277f
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Establishment of a novel human lymphoblastic cell strain with the long arm of chromosome 11 aberration without MLL rearrangement

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