Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22

Abstract Comparative characterization of microRNA-mediated stress regulatory networks in contrasting rice cultivars is critical to decipher plant stress response. Consequently, a multi-level comparative analysis, using sRNA sequencing, degradome analysis, enzymatic and metabolite assays and metal io...

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Autores principales:	Sonia Balyan, Mukesh Kumar, Roseeta Devi Mutum, Utkarsh Raghuvanshi, Priyanka Agarwal, Saloni Mathur, Saurabh Raghuvanshi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9addf71e9df44fc99da667f0ee67f687
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spelling	oai:doaj.org-article:9addf71e9df44fc99da667f0ee67f6872021-12-02T15:04:57ZIdentification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 2210.1038/s41598-017-15450-12045-2322https://doaj.org/article/9addf71e9df44fc99da667f0ee67f6872017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-15450-1https://doaj.org/toc/2045-2322Abstract Comparative characterization of microRNA-mediated stress regulatory networks in contrasting rice cultivars is critical to decipher plant stress response. Consequently, a multi-level comparative analysis, using sRNA sequencing, degradome analysis, enzymatic and metabolite assays and metal ion analysis, in drought tolerant and sensitive rice cultivars was conducted. The study identified a group of miRNAs “Cultivar-specific drought responsive” (CSDR)-miRNAs (osa-miR159f, osa-miR1871, osa-miR398b, osa-miR408-3p, osa-miR2878-5p, osa-miR528-5p and osa-miR397a) that were up-regulated in the flag-leaves of tolerant cultivar, Nagina 22 (N22) and Vandana, but down-regulated in the sensitive cultivar, Pusa Basmati 1 (PB1) and IR64, during drought. Interestingly, CSDR-miRNAs target several copper-protein coding transcripts like plantacyanins, laccases and Copper/Zinc superoxide dismutases (Cu/Zn SODs) and are themselves found to be similarly induced under simulated copper-starvation in both N22 and PB1. Transcription factor OsSPL9, implicated in Cu-homeostasis also interacted with osa-miR408-3p and osa-miR528-5p promoters. Further, N22 flag leaves showed lower SOD activity, accumulated ROS and had a higher stomata closure. Interestingly, compared to PB1, internal Cu levels significantly decreased in the N22 flag-leaves, during drought. Thus, the study identifies the unique drought mediated dynamism and interplay of Cu and ROS homeostasis, in the flag leaves of drought tolerant rice, wherein CSDR-miRNAs play a pivotal role.Sonia BalyanMukesh KumarRoseeta Devi MutumUtkarsh RaghuvanshiPriyanka AgarwalSaloni MathurSaurabh RaghuvanshiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sonia Balyan Mukesh Kumar Roseeta Devi Mutum Utkarsh Raghuvanshi Priyanka Agarwal Saloni Mathur Saurabh Raghuvanshi Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22
description	Abstract Comparative characterization of microRNA-mediated stress regulatory networks in contrasting rice cultivars is critical to decipher plant stress response. Consequently, a multi-level comparative analysis, using sRNA sequencing, degradome analysis, enzymatic and metabolite assays and metal ion analysis, in drought tolerant and sensitive rice cultivars was conducted. The study identified a group of miRNAs “Cultivar-specific drought responsive” (CSDR)-miRNAs (osa-miR159f, osa-miR1871, osa-miR398b, osa-miR408-3p, osa-miR2878-5p, osa-miR528-5p and osa-miR397a) that were up-regulated in the flag-leaves of tolerant cultivar, Nagina 22 (N22) and Vandana, but down-regulated in the sensitive cultivar, Pusa Basmati 1 (PB1) and IR64, during drought. Interestingly, CSDR-miRNAs target several copper-protein coding transcripts like plantacyanins, laccases and Copper/Zinc superoxide dismutases (Cu/Zn SODs) and are themselves found to be similarly induced under simulated copper-starvation in both N22 and PB1. Transcription factor OsSPL9, implicated in Cu-homeostasis also interacted with osa-miR408-3p and osa-miR528-5p promoters. Further, N22 flag leaves showed lower SOD activity, accumulated ROS and had a higher stomata closure. Interestingly, compared to PB1, internal Cu levels significantly decreased in the N22 flag-leaves, during drought. Thus, the study identifies the unique drought mediated dynamism and interplay of Cu and ROS homeostasis, in the flag leaves of drought tolerant rice, wherein CSDR-miRNAs play a pivotal role.
format	article
author	Sonia Balyan Mukesh Kumar Roseeta Devi Mutum Utkarsh Raghuvanshi Priyanka Agarwal Saloni Mathur Saurabh Raghuvanshi
author_facet	Sonia Balyan Mukesh Kumar Roseeta Devi Mutum Utkarsh Raghuvanshi Priyanka Agarwal Saloni Mathur Saurabh Raghuvanshi
author_sort	Sonia Balyan
title	Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22
title_short	Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22
title_full	Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22
title_fullStr	Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22
title_full_unstemmed	Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22
title_sort	identification of mirna-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, nagina 22
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9addf71e9df44fc99da667f0ee67f687
work_keys_str_mv	AT soniabalyan identificationofmirnamediateddroughtresponsivemultitieredregulatorynetworkindroughttolerantricenagina22 AT mukeshkumar identificationofmirnamediateddroughtresponsivemultitieredregulatorynetworkindroughttolerantricenagina22 AT roseetadevimutum identificationofmirnamediateddroughtresponsivemultitieredregulatorynetworkindroughttolerantricenagina22 AT utkarshraghuvanshi identificationofmirnamediateddroughtresponsivemultitieredregulatorynetworkindroughttolerantricenagina22 AT priyankaagarwal identificationofmirnamediateddroughtresponsivemultitieredregulatorynetworkindroughttolerantricenagina22 AT salonimathur identificationofmirnamediateddroughtresponsivemultitieredregulatorynetworkindroughttolerantricenagina22 AT saurabhraghuvanshi identificationofmirnamediateddroughtresponsivemultitieredregulatorynetworkindroughttolerantricenagina22
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Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22

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