Identification of a New Cyclovirus in Cerebrospinal Fluid of Patients with Acute Central Nervous System Infections
ABSTRACT Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology remains unknown in a large proportion of cases. We identified and characterized the full genome of a novel cyclovirus (tentatively named cyclovirus-Vietnam [CyCV-VN]) in cerebrospinal f...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
American Society for Microbiology
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9ae36551c31642fbb3ba20b9bf8474d6 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:9ae36551c31642fbb3ba20b9bf8474d6 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:9ae36551c31642fbb3ba20b9bf8474d62021-11-15T15:40:06ZIdentification of a New Cyclovirus in Cerebrospinal Fluid of Patients with Acute Central Nervous System Infections10.1128/mBio.00231-132150-7511https://doaj.org/article/9ae36551c31642fbb3ba20b9bf8474d62013-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00231-13https://doaj.org/toc/2150-7511ABSTRACT Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology remains unknown in a large proportion of cases. We identified and characterized the full genome of a novel cyclovirus (tentatively named cyclovirus-Vietnam [CyCV-VN]) in cerebrospinal fluid (CSF) specimens of two Vietnamese patients with CNS infections of unknown etiology. CyCV-VN was subsequently detected in 4% of 642 CSF specimens from Vietnamese patients with suspected CNS infections and none of 122 CSFs from patients with noninfectious neurological disorders. Detection rates were similar in patients with CNS infections of unknown etiology and those in whom other pathogens were detected. A similar detection rate in feces from healthy children suggested food-borne or orofecal transmission routes, while high detection rates in feces from pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further research is needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus. IMPORTANCE Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology frequently remains unknown, which hampers development of therapeutic or preventive strategies. Hence, identification of novel pathogens is essential and is facilitated by current next-generation sequencing-based methods. Using such technology, we identified and characterized the full genome of a novel cyclovirus in cerebrospinal fluid (CSF) specimens from two Vietnamese patients with CNS infections of unknown etiology, which was subsequently detected in none of 122 CSF specimens from patients with noninfectious neurological disorders but 4% of 642 CSF specimens from Vietnamese patients with suspected or confirmed CNS infections. Similar detection rates in feces from healthy children suggested food-borne or orofecal transmission routes, while frequent detection in feces from Vietnamese pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further studies are needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus.Le Van TanH. Rogier van DoornHo Dang Trung NghiaTran Thi Hong ChauLe Thi Phuong TuMichel de VriesMarta CanutiMartin DeijsMaarten F. JebbinkStephen BakerJuliet E. BryantNguyen Thi ThamNguyen Thi Thuy Chinh BKrongMaciej F. BoniTran Quoc LoiLe Thi PhuongJoost T. P. VerhoevenMartin CrusatRienk E. JeeningaConstance SchultszNguyen Van Vinh ChauTran Tinh HienLia van der HoekJeremy FarrarMenno D. de JongAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 3 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Microbiology QR1-502 |
| spellingShingle |
Microbiology QR1-502 Le Van Tan H. Rogier van Doorn Ho Dang Trung Nghia Tran Thi Hong Chau Le Thi Phuong Tu Michel de Vries Marta Canuti Martin Deijs Maarten F. Jebbink Stephen Baker Juliet E. Bryant Nguyen Thi Tham Nguyen Thi Thuy Chinh BKrong Maciej F. Boni Tran Quoc Loi Le Thi Phuong Joost T. P. Verhoeven Martin Crusat Rienk E. Jeeninga Constance Schultsz Nguyen Van Vinh Chau Tran Tinh Hien Lia van der Hoek Jeremy Farrar Menno D. de Jong Identification of a New Cyclovirus in Cerebrospinal Fluid of Patients with Acute Central Nervous System Infections |
| description |
ABSTRACT Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology remains unknown in a large proportion of cases. We identified and characterized the full genome of a novel cyclovirus (tentatively named cyclovirus-Vietnam [CyCV-VN]) in cerebrospinal fluid (CSF) specimens of two Vietnamese patients with CNS infections of unknown etiology. CyCV-VN was subsequently detected in 4% of 642 CSF specimens from Vietnamese patients with suspected CNS infections and none of 122 CSFs from patients with noninfectious neurological disorders. Detection rates were similar in patients with CNS infections of unknown etiology and those in whom other pathogens were detected. A similar detection rate in feces from healthy children suggested food-borne or orofecal transmission routes, while high detection rates in feces from pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further research is needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus. IMPORTANCE Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology frequently remains unknown, which hampers development of therapeutic or preventive strategies. Hence, identification of novel pathogens is essential and is facilitated by current next-generation sequencing-based methods. Using such technology, we identified and characterized the full genome of a novel cyclovirus in cerebrospinal fluid (CSF) specimens from two Vietnamese patients with CNS infections of unknown etiology, which was subsequently detected in none of 122 CSF specimens from patients with noninfectious neurological disorders but 4% of 642 CSF specimens from Vietnamese patients with suspected or confirmed CNS infections. Similar detection rates in feces from healthy children suggested food-borne or orofecal transmission routes, while frequent detection in feces from Vietnamese pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further studies are needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus. |
| format |
article |
| author |
Le Van Tan H. Rogier van Doorn Ho Dang Trung Nghia Tran Thi Hong Chau Le Thi Phuong Tu Michel de Vries Marta Canuti Martin Deijs Maarten F. Jebbink Stephen Baker Juliet E. Bryant Nguyen Thi Tham Nguyen Thi Thuy Chinh BKrong Maciej F. Boni Tran Quoc Loi Le Thi Phuong Joost T. P. Verhoeven Martin Crusat Rienk E. Jeeninga Constance Schultsz Nguyen Van Vinh Chau Tran Tinh Hien Lia van der Hoek Jeremy Farrar Menno D. de Jong |
| author_facet |
Le Van Tan H. Rogier van Doorn Ho Dang Trung Nghia Tran Thi Hong Chau Le Thi Phuong Tu Michel de Vries Marta Canuti Martin Deijs Maarten F. Jebbink Stephen Baker Juliet E. Bryant Nguyen Thi Tham Nguyen Thi Thuy Chinh BKrong Maciej F. Boni Tran Quoc Loi Le Thi Phuong Joost T. P. Verhoeven Martin Crusat Rienk E. Jeeninga Constance Schultsz Nguyen Van Vinh Chau Tran Tinh Hien Lia van der Hoek Jeremy Farrar Menno D. de Jong |
| author_sort |
Le Van Tan |
| title |
Identification of a New Cyclovirus in Cerebrospinal Fluid of Patients with Acute Central Nervous System Infections |
| title_short |
Identification of a New Cyclovirus in Cerebrospinal Fluid of Patients with Acute Central Nervous System Infections |
| title_full |
Identification of a New Cyclovirus in Cerebrospinal Fluid of Patients with Acute Central Nervous System Infections |
| title_fullStr |
Identification of a New Cyclovirus in Cerebrospinal Fluid of Patients with Acute Central Nervous System Infections |
| title_full_unstemmed |
Identification of a New Cyclovirus in Cerebrospinal Fluid of Patients with Acute Central Nervous System Infections |
| title_sort |
identification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections |
| publisher |
American Society for Microbiology |
| publishDate |
2013 |
| url |
https://doaj.org/article/9ae36551c31642fbb3ba20b9bf8474d6 |
| work_keys_str_mv |
AT levantan identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT hrogiervandoorn identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT hodangtrungnghia identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT tranthihongchau identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT lethiphuongtu identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT micheldevries identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT martacanuti identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT martindeijs identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT maartenfjebbink identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT stephenbaker identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT julietebryant identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT nguyenthitham identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT nguyenthithuychinhbkrong identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT maciejfboni identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT tranquocloi identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT lethiphuong identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT joosttpverhoeven identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT martincrusat identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT rienkejeeninga identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT constanceschultsz identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT nguyenvanvinhchau identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT trantinhhien identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT liavanderhoek identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT jeremyfarrar identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections AT mennoddejong identificationofanewcyclovirusincerebrospinalfluidofpatientswithacutecentralnervoussysteminfections |
| _version_ |
1718427781882183680 |