A simple method for isolation of rest of trypsinized stem cells with magnetic beads

Till now, there is no laboratory in the world, which makes thoughts that there may be still stem cells remaining in the flasks after the trypsin treatment. The user checks the presence of stem cells during trypsinization with the microscope whether the whole population of stem cells has been collect...

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Autor principal:	Sudhir Bhatia
Formato:	article
Lenguaje:	ID
Publicado:	Poltekkes Kemenkes Yogyakarta 2021
Materias:	trypsinized stem cells magnetic beads Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9af1b4f6fd7041e8b0d0c1663db3d6c6
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spelling	oai:doaj.org-article:9af1b4f6fd7041e8b0d0c1663db3d6c62021-11-13T13:29:49ZA simple method for isolation of rest of trypsinized stem cells with magnetic beads2338-56342580-019110.29238/teknolabjournal.v10i1.268https://doaj.org/article/9af1b4f6fd7041e8b0d0c1663db3d6c62021-06-01T00:00:00Zhttps://teknolabjournal.com/index.php/Jtl/article/view/268https://doaj.org/toc/2338-5634https://doaj.org/toc/2580-0191Till now, there is no laboratory in the world, which makes thoughts that there may be still stem cells remaining in the flasks after the trypsin treatment. The user checks the presence of stem cells during trypsinization with the microscope whether the whole population of stem cells has been collected. Magnetic beads are being used around the world to isolate different kinds of cells like CD4, CD8, CD34, and other cells. One of the advantages of magnetic isolation is that they can be used to isolate cells from solutions containing even a low number of targeted cells. We conducted the experiments to see whether all remaining MSC can be obtained from a trypsin-treated flask with magnetic beads isolation. During the magnetic isolation with CD90 specific Magnetic beads, it was found under the microscope that there are huge numbers of cells being attached to beads. The results indicate that there are still a huge number of rest cells in the trypsinized flask but most of the users think that they have isolated all cells, which is not true and they are throwing away valuable stem cells. The magnetic beads can be used to isolate the rest of MSC because for many applications, there is a need of the largest number of stem cells for conducting studies like flow cytometry, molecular testing along with pre-clinical and clinical studies.Sudhir BhatiaPoltekkes Kemenkes Yogyakartaarticletrypsinizedstem cellsmagnetic beadsMedicine (General)R5-920IDJurnal Teknologi Laboratorium, Vol 10, Iss 1, Pp 01-02 (2021)
institution	DOAJ
collection	DOAJ
language	ID
topic	trypsinized stem cells magnetic beads Medicine (General) R5-920
spellingShingle	trypsinized stem cells magnetic beads Medicine (General) R5-920 Sudhir Bhatia A simple method for isolation of rest of trypsinized stem cells with magnetic beads
description	Till now, there is no laboratory in the world, which makes thoughts that there may be still stem cells remaining in the flasks after the trypsin treatment. The user checks the presence of stem cells during trypsinization with the microscope whether the whole population of stem cells has been collected. Magnetic beads are being used around the world to isolate different kinds of cells like CD4, CD8, CD34, and other cells. One of the advantages of magnetic isolation is that they can be used to isolate cells from solutions containing even a low number of targeted cells. We conducted the experiments to see whether all remaining MSC can be obtained from a trypsin-treated flask with magnetic beads isolation. During the magnetic isolation with CD90 specific Magnetic beads, it was found under the microscope that there are huge numbers of cells being attached to beads. The results indicate that there are still a huge number of rest cells in the trypsinized flask but most of the users think that they have isolated all cells, which is not true and they are throwing away valuable stem cells. The magnetic beads can be used to isolate the rest of MSC because for many applications, there is a need of the largest number of stem cells for conducting studies like flow cytometry, molecular testing along with pre-clinical and clinical studies.
format	article
author	Sudhir Bhatia
author_facet	Sudhir Bhatia
author_sort	Sudhir Bhatia
title	A simple method for isolation of rest of trypsinized stem cells with magnetic beads
title_short	A simple method for isolation of rest of trypsinized stem cells with magnetic beads
title_full	A simple method for isolation of rest of trypsinized stem cells with magnetic beads
title_fullStr	A simple method for isolation of rest of trypsinized stem cells with magnetic beads
title_full_unstemmed	A simple method for isolation of rest of trypsinized stem cells with magnetic beads
title_sort	simple method for isolation of rest of trypsinized stem cells with magnetic beads
publisher	Poltekkes Kemenkes Yogyakarta
publishDate	2021
url	https://doaj.org/article/9af1b4f6fd7041e8b0d0c1663db3d6c6
work_keys_str_mv	AT sudhirbhatia asimplemethodforisolationofrestoftrypsinizedstemcellswithmagneticbeads AT sudhirbhatia simplemethodforisolationofrestoftrypsinizedstemcellswithmagneticbeads
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A simple method for isolation of rest of trypsinized stem cells with magnetic beads

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