The Identified Hub Gene GlcN in Osteoarthritis Progression and Treatment

Background. As a chronic disease, osteoarthritis has caused great trouble to the health of middle-aged and elderly people. Studies have shown that glucosamine (GlcN) can be used to abate the progression and improve this disease. Based on this point of view, we try to verify the connection between Gl...

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Autores principales:	Jingsheng Liu, Xiaoli Dong, Yining Liu, Kai Wang, Shuanhu Lei, Mingxuan Yang, Haiyuan Yue, Haijun Feng, Kai Feng, Kang Li, Jianwei Zhou, Yanqiang Chen, Wenjia Du, Xuewen Kang, Yayi Xia
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Computer applications to medicine. Medical informatics R858-859.7
Acceso en línea:	https://doaj.org/article/9af684742ad84cbf91a1e77b8f25f7c5
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spelling	oai:doaj.org-article:9af684742ad84cbf91a1e77b8f25f7c52021-11-08T02:36:29ZThe Identified Hub Gene GlcN in Osteoarthritis Progression and Treatment1748-671810.1155/2021/5499450https://doaj.org/article/9af684742ad84cbf91a1e77b8f25f7c52021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/5499450https://doaj.org/toc/1748-6718Background. As a chronic disease, osteoarthritis has caused great trouble to the health of middle-aged and elderly people. Studies have shown that glucosamine (GlcN) can be used to abate the progression and improve this disease. Based on this point of view, we try to verify the connection between GlcN and osteoarthritis and find more effective biomarkers. Methods. We downloaded the GSE72575 data set from the GEO database, and used the R language to perform DEG analysis on the gene expression profile of the samples. Next, the GO function and the KEGG signaling pathways were analyzed through the DAVID database, and then, the KEGG pathways enriched in the gene set were analyzed based on GSEA. Then, the PPI network of DEGs was constructed based on the STRING online database, and finally, the hub genes were selected by Cytoscape. Results. Three GlcN-treated MH7A cell treatment groups and 3 control groups in the GSE72575 data set were studied. Through analysis, there were 52 DEGs in these samples. Then, through GO, KEGG, and GSEA, regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway, FoxO signaling pathway, JAK-STAT signaling pathway, PI3K-Akt signaling pathway, TGF-beta signaling pathway, and ECM receptor interaction were involved in the regulatory mechanisms of the osteoarthritis pathogenesis. After that, the hub genes IL6 and DDIT3 were identified through PPI network construction and analysis. And it was found that IL6 was lowly expressed in the group with GlcN-treated MH7A cells, while DDIT3 was highly expressed. Conclusion. The above results provide a basis for GlcN to participate in the treatment of osteoarthritis and a possibility for finding effective therapeutic targets.Jingsheng LiuXiaoli DongYining LiuKai WangShuanhu LeiMingxuan YangHaiyuan YueHaijun FengKai FengKang LiJianwei ZhouYanqiang ChenWenjia DuXuewen KangYayi XiaHindawi LimitedarticleComputer applications to medicine. Medical informaticsR858-859.7ENComputational and Mathematical Methods in Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Computer applications to medicine. Medical informatics R858-859.7
spellingShingle	Computer applications to medicine. Medical informatics R858-859.7 Jingsheng Liu Xiaoli Dong Yining Liu Kai Wang Shuanhu Lei Mingxuan Yang Haiyuan Yue Haijun Feng Kai Feng Kang Li Jianwei Zhou Yanqiang Chen Wenjia Du Xuewen Kang Yayi Xia The Identified Hub Gene GlcN in Osteoarthritis Progression and Treatment
description	Background. As a chronic disease, osteoarthritis has caused great trouble to the health of middle-aged and elderly people. Studies have shown that glucosamine (GlcN) can be used to abate the progression and improve this disease. Based on this point of view, we try to verify the connection between GlcN and osteoarthritis and find more effective biomarkers. Methods. We downloaded the GSE72575 data set from the GEO database, and used the R language to perform DEG analysis on the gene expression profile of the samples. Next, the GO function and the KEGG signaling pathways were analyzed through the DAVID database, and then, the KEGG pathways enriched in the gene set were analyzed based on GSEA. Then, the PPI network of DEGs was constructed based on the STRING online database, and finally, the hub genes were selected by Cytoscape. Results. Three GlcN-treated MH7A cell treatment groups and 3 control groups in the GSE72575 data set were studied. Through analysis, there were 52 DEGs in these samples. Then, through GO, KEGG, and GSEA, regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway, FoxO signaling pathway, JAK-STAT signaling pathway, PI3K-Akt signaling pathway, TGF-beta signaling pathway, and ECM receptor interaction were involved in the regulatory mechanisms of the osteoarthritis pathogenesis. After that, the hub genes IL6 and DDIT3 were identified through PPI network construction and analysis. And it was found that IL6 was lowly expressed in the group with GlcN-treated MH7A cells, while DDIT3 was highly expressed. Conclusion. The above results provide a basis for GlcN to participate in the treatment of osteoarthritis and a possibility for finding effective therapeutic targets.
format	article
author	Jingsheng Liu Xiaoli Dong Yining Liu Kai Wang Shuanhu Lei Mingxuan Yang Haiyuan Yue Haijun Feng Kai Feng Kang Li Jianwei Zhou Yanqiang Chen Wenjia Du Xuewen Kang Yayi Xia
author_facet	Jingsheng Liu Xiaoli Dong Yining Liu Kai Wang Shuanhu Lei Mingxuan Yang Haiyuan Yue Haijun Feng Kai Feng Kang Li Jianwei Zhou Yanqiang Chen Wenjia Du Xuewen Kang Yayi Xia
author_sort	Jingsheng Liu
title	The Identified Hub Gene GlcN in Osteoarthritis Progression and Treatment
title_short	The Identified Hub Gene GlcN in Osteoarthritis Progression and Treatment
title_full	The Identified Hub Gene GlcN in Osteoarthritis Progression and Treatment
title_fullStr	The Identified Hub Gene GlcN in Osteoarthritis Progression and Treatment
title_full_unstemmed	The Identified Hub Gene GlcN in Osteoarthritis Progression and Treatment
title_sort	identified hub gene glcn in osteoarthritis progression and treatment
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/9af684742ad84cbf91a1e77b8f25f7c5
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The Identified Hub Gene GlcN in Osteoarthritis Progression and Treatment

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