Genome-Wide Identification and Characterization of Soybean GmLOR Gene Family and Expression Analysis in Response to Abiotic Stresses

The LOR (LURP-one related) family genes encode proteins containing a conserved LOR domain. Several members of the LOR family genes are required for defense against Hyaloperonospora parasitica (Hpa) in Arabidopsis. However, there are few report...

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Autores principales:	Yisheng Fang, Dong Cao, Hongli Yang, Wei Guo, Wenqi Ouyang, Haifeng Chen, Zhihui Shan, Zhonglu Yang, Shuilian Chen, Xia Li, Limiao Chen, Xinan Zhou
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	soybean LOR gene family phylogenetic analysis gene expression osmotic stress salt stress Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/9b04fe7e54a94d6796a1fe8c338e8515
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spelling	oai:doaj.org-article:9b04fe7e54a94d6796a1fe8c338e85152021-11-25T17:57:25ZGenome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses10.3390/ijms2222125151422-00671661-6596https://doaj.org/article/9b04fe7e54a94d6796a1fe8c338e85152021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12515https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The LOR (<i>LURP</i>-one related) family genes encode proteins containing a conserved LOR domain. Several members of the LOR family genes are required for defense against <i>Hyaloperonospora parasitica</i> (<i>Hpa</i>) in Arabidopsis. However, there are few reports of LOR genes in response to abiotic stresses in plants. In this study, a genome-wide survey and expression levels in response to abiotic stresses of 36 LOR genes from <i>Glycine max</i> were conducted. The results indicated that the <i>GmLOR</i> gene family was divided into eight subgroups, distributed on 14 chromosomes. A majority of members contained three extremely conservative motifs. There were four pairs of tandem duplicated <i>GmLORs</i> and nineteen pairs of segmental duplicated genes identified, which led to the expansion of the number of <i>GmLOR</i> genes. The expansion patterns of the <i>GmLOR</i> family were mainly segmental duplication. A heatmap of soybean LOR family genes showed that 36 <i>GmLOR</i> genes exhibited various expression patterns in different tissues. The cis-acting elements in promoter regions of <i>GmLORs</i> include abiotic stress-responsive elements, such as dehydration-responsive elements and drought-inducible elements. Real-time quantitative PCR was used to detect the expression level of <i>GmLOR</i> genes, and most of them were expressed in the leaf or root except that <i>GmLOR6</i> was induced by osmotic and salt stresses. Moreover, <i>GmLOR4/10/14/19</i> were significantly upregulated after PEG and salt treatments, indicating important roles in the improvement of plant tolerance to abiotic stress. Overall, our study provides a foundation for future investigations of <i>GmLOR</i> gene functions in soybean.Yisheng FangDong CaoHongli YangWei GuoWenqi OuyangHaifeng ChenZhihui ShanZhonglu YangShuilian ChenXia LiLimiao ChenXinan ZhouMDPI AGarticlesoybeanLOR gene familyphylogenetic analysisgene expressionosmotic stresssalt stressBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12515, p 12515 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	soybean LOR gene family phylogenetic analysis gene expression osmotic stress salt stress Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	soybean LOR gene family phylogenetic analysis gene expression osmotic stress salt stress Biology (General) QH301-705.5 Chemistry QD1-999 Yisheng Fang Dong Cao Hongli Yang Wei Guo Wenqi Ouyang Haifeng Chen Zhihui Shan Zhonglu Yang Shuilian Chen Xia Li Limiao Chen Xinan Zhou Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses
description	The LOR (<i>LURP</i>-one related) family genes encode proteins containing a conserved LOR domain. Several members of the LOR family genes are required for defense against <i>Hyaloperonospora parasitica</i> (<i>Hpa</i>) in Arabidopsis. However, there are few reports of LOR genes in response to abiotic stresses in plants. In this study, a genome-wide survey and expression levels in response to abiotic stresses of 36 LOR genes from <i>Glycine max</i> were conducted. The results indicated that the <i>GmLOR</i> gene family was divided into eight subgroups, distributed on 14 chromosomes. A majority of members contained three extremely conservative motifs. There were four pairs of tandem duplicated <i>GmLORs</i> and nineteen pairs of segmental duplicated genes identified, which led to the expansion of the number of <i>GmLOR</i> genes. The expansion patterns of the <i>GmLOR</i> family were mainly segmental duplication. A heatmap of soybean LOR family genes showed that 36 <i>GmLOR</i> genes exhibited various expression patterns in different tissues. The cis-acting elements in promoter regions of <i>GmLORs</i> include abiotic stress-responsive elements, such as dehydration-responsive elements and drought-inducible elements. Real-time quantitative PCR was used to detect the expression level of <i>GmLOR</i> genes, and most of them were expressed in the leaf or root except that <i>GmLOR6</i> was induced by osmotic and salt stresses. Moreover, <i>GmLOR4/10/14/19</i> were significantly upregulated after PEG and salt treatments, indicating important roles in the improvement of plant tolerance to abiotic stress. Overall, our study provides a foundation for future investigations of <i>GmLOR</i> gene functions in soybean.
format	article
author	Yisheng Fang Dong Cao Hongli Yang Wei Guo Wenqi Ouyang Haifeng Chen Zhihui Shan Zhonglu Yang Shuilian Chen Xia Li Limiao Chen Xinan Zhou
author_facet	Yisheng Fang Dong Cao Hongli Yang Wei Guo Wenqi Ouyang Haifeng Chen Zhihui Shan Zhonglu Yang Shuilian Chen Xia Li Limiao Chen Xinan Zhou
author_sort	Yisheng Fang
title	Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses
title_short	Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses
title_full	Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses
title_fullStr	Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses
title_full_unstemmed	Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses
title_sort	genome-wide identification and characterization of soybean <i>gmlor</i> gene family and expression analysis in response to abiotic stresses
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9b04fe7e54a94d6796a1fe8c338e8515
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Genome-Wide Identification and Characterization of Soybean <i>GmLOR</i> Gene Family and Expression Analysis in Response to Abiotic Stresses

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