Molecular imaging can identify the location to perform a frozen biopsy during intraoperative frozen section consultation.

<h4>Background</h4>Intraoperative frozen section (FS) consultation is an important tool in surgical oncology that suffers from sampling error because the pathologist does not always know where to perform a biopsy of the surgical specimen. Intraoperative molecular imaging is a technology...

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Autores principales:	Mitchell G Bryski, Lydia G Frenzel-Sulyok, E James Delikatny, Charuhas Deshpande, Leslie A Litzky, Sunil Singhal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b085d30b461440480281eb3e77ba32f
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spelling	oai:doaj.org-article:9b085d30b461440480281eb3e77ba32f2021-12-02T20:07:16ZMolecular imaging can identify the location to perform a frozen biopsy during intraoperative frozen section consultation.1932-620310.1371/journal.pone.0252731https://doaj.org/article/9b085d30b461440480281eb3e77ba32f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252731https://doaj.org/toc/1932-6203<h4>Background</h4>Intraoperative frozen section (FS) consultation is an important tool in surgical oncology that suffers from sampling error because the pathologist does not always know where to perform a biopsy of the surgical specimen. Intraoperative molecular imaging is a technology used in the OR to visualize lesions during surgery. We hypothesized that molecular imaging can address this pathology challenge in FS by visualizing the cancer cells in the specimen in the pathology suite. Here, we report the development and validation of a molecular-imaging capable cryostat called Smart-Cut.<h4>Methods</h4>A molecular imaging capable cryostat prototype was developed and tested using a murine model. Tumors grown in mice were targeted with a NIR contrast agent, indocyanine green (ICG), via tail vein injection. Tumors and adjacent normal tissue samples were frozen sectioned with Smart-Cut. Fluorescent sections and non-fluorescent sections were prepared for H&E and fluorescent microscopy. Fluorescent signal was quantified by tumor-to-background ratio (TBR). NIR fluorescence was tested in one patient enrolled in a clinical trial.<h4>Results</h4>The Smart-Cut prototype has a small footprint and fits well in the pathology suite. Fluorescence imaging with Smart-Cut identified cancerous tissue in the specimen in all 12 mice. No false positives or false negatives were seen, as confirmed by H&E. The mean TBR in Smart-Cut positive tissue sections was 6.8 (SD±3.8). In a clinical application in the pathology suite, NIR imaging identified two lesions in a pulmonary resection specimen, where traditional grossing only identified one.<h4>Conclusion</h4>Molecular imaging can be integrated into the pathology suite via the Smart-Cut device, and can detect cancer in frozen tissue sections using molecular imaging in a murine model.Mitchell G BryskiLydia G Frenzel-SulyokE James DelikatnyCharuhas DeshpandeLeslie A LitzkySunil SinghalPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252731 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mitchell G Bryski Lydia G Frenzel-Sulyok E James Delikatny Charuhas Deshpande Leslie A Litzky Sunil Singhal Molecular imaging can identify the location to perform a frozen biopsy during intraoperative frozen section consultation.
description	<h4>Background</h4>Intraoperative frozen section (FS) consultation is an important tool in surgical oncology that suffers from sampling error because the pathologist does not always know where to perform a biopsy of the surgical specimen. Intraoperative molecular imaging is a technology used in the OR to visualize lesions during surgery. We hypothesized that molecular imaging can address this pathology challenge in FS by visualizing the cancer cells in the specimen in the pathology suite. Here, we report the development and validation of a molecular-imaging capable cryostat called Smart-Cut.<h4>Methods</h4>A molecular imaging capable cryostat prototype was developed and tested using a murine model. Tumors grown in mice were targeted with a NIR contrast agent, indocyanine green (ICG), via tail vein injection. Tumors and adjacent normal tissue samples were frozen sectioned with Smart-Cut. Fluorescent sections and non-fluorescent sections were prepared for H&E and fluorescent microscopy. Fluorescent signal was quantified by tumor-to-background ratio (TBR). NIR fluorescence was tested in one patient enrolled in a clinical trial.<h4>Results</h4>The Smart-Cut prototype has a small footprint and fits well in the pathology suite. Fluorescence imaging with Smart-Cut identified cancerous tissue in the specimen in all 12 mice. No false positives or false negatives were seen, as confirmed by H&E. The mean TBR in Smart-Cut positive tissue sections was 6.8 (SD±3.8). In a clinical application in the pathology suite, NIR imaging identified two lesions in a pulmonary resection specimen, where traditional grossing only identified one.<h4>Conclusion</h4>Molecular imaging can be integrated into the pathology suite via the Smart-Cut device, and can detect cancer in frozen tissue sections using molecular imaging in a murine model.
format	article
author	Mitchell G Bryski Lydia G Frenzel-Sulyok E James Delikatny Charuhas Deshpande Leslie A Litzky Sunil Singhal
author_facet	Mitchell G Bryski Lydia G Frenzel-Sulyok E James Delikatny Charuhas Deshpande Leslie A Litzky Sunil Singhal
author_sort	Mitchell G Bryski
title	Molecular imaging can identify the location to perform a frozen biopsy during intraoperative frozen section consultation.
title_short	Molecular imaging can identify the location to perform a frozen biopsy during intraoperative frozen section consultation.
title_full	Molecular imaging can identify the location to perform a frozen biopsy during intraoperative frozen section consultation.
title_fullStr	Molecular imaging can identify the location to perform a frozen biopsy during intraoperative frozen section consultation.
title_full_unstemmed	Molecular imaging can identify the location to perform a frozen biopsy during intraoperative frozen section consultation.
title_sort	molecular imaging can identify the location to perform a frozen biopsy during intraoperative frozen section consultation.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/9b085d30b461440480281eb3e77ba32f
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Molecular imaging can identify the location to perform a frozen biopsy during intraoperative frozen section consultation.

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