The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region

Abstract Jaw1/LRMP is a type II integral membrane protein that is localized at the endoplasmic reticulum (ER) and outer nuclear membrane. We previously reported that a function of Jaw1 is to maintain the nuclear shape as a KASH protein via its carboxyl terminal region, a component of linker of nucle...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Takuma Kozono, Hiroyuki Sato, Wataru Okumura, Chifuyu Jogano, Miwa Tamura-Nakano, Yuki I. Kawamura, Jack Rohrer, Takashi Tonozuka, Atsushi Nishikawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b0bbb77d4e84230962f6a8b257e98b9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9b0bbb77d4e84230962f6a8b257e98b9
record_format	dspace
spelling	oai:doaj.org-article:9b0bbb77d4e84230962f6a8b257e98b92021-12-02T14:12:40ZThe N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region10.1038/s41598-020-80258-52045-2322https://doaj.org/article/9b0bbb77d4e84230962f6a8b257e98b92021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80258-5https://doaj.org/toc/2045-2322Abstract Jaw1/LRMP is a type II integral membrane protein that is localized at the endoplasmic reticulum (ER) and outer nuclear membrane. We previously reported that a function of Jaw1 is to maintain the nuclear shape as a KASH protein via its carboxyl terminal region, a component of linker of nucleoskeleton and cytoskeleton complex in the oligomeric state. Although the oligomerization of some KASH proteins via the cytosolic regions serves to stabilize protein-protein interactions, the issue of how the oligomerization of Jaw1 is regulated is not completely understood. Therefore, we focused on three distinct regions on the cytosolic face of Jaw1: the N-terminal region, the coiled-coil domain and the stem region, in terms of oligomerization. A co-immunoprecipitation assay showed that its coiled-coil domain is a candidate for the oligomerization site. Furthermore, our data indicated that the N-terminal region prevents the aberrant oligomerization of Jaw1 as an intrinsically disordered region (IDR). Importantly, the ectopic expression of an N-terminal region deleted mutant caused the formation of organized smooth ER (OSER), structures such as nuclear karmellae and whorls, in B16F10 cells. Furthermore, this OSER interfered with the localization of the oligomer and interactors such as the type III inositol 1,4,5-triphosphate receptor (IP3R3) and SUN2. In summary, the N-terminal region of Jaw1 inhibits the formation of OSER as an IDR to maintain the homeostatic localization of interactors on the ER membrane.Takuma KozonoHiroyuki SatoWataru OkumuraChifuyu JoganoMiwa Tamura-NakanoYuki I. KawamuraJack RohrerTakashi TonozukaAtsushi NishikawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takuma Kozono Hiroyuki Sato Wataru Okumura Chifuyu Jogano Miwa Tamura-Nakano Yuki I. Kawamura Jack Rohrer Takashi Tonozuka Atsushi Nishikawa The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region
description	Abstract Jaw1/LRMP is a type II integral membrane protein that is localized at the endoplasmic reticulum (ER) and outer nuclear membrane. We previously reported that a function of Jaw1 is to maintain the nuclear shape as a KASH protein via its carboxyl terminal region, a component of linker of nucleoskeleton and cytoskeleton complex in the oligomeric state. Although the oligomerization of some KASH proteins via the cytosolic regions serves to stabilize protein-protein interactions, the issue of how the oligomerization of Jaw1 is regulated is not completely understood. Therefore, we focused on three distinct regions on the cytosolic face of Jaw1: the N-terminal region, the coiled-coil domain and the stem region, in terms of oligomerization. A co-immunoprecipitation assay showed that its coiled-coil domain is a candidate for the oligomerization site. Furthermore, our data indicated that the N-terminal region prevents the aberrant oligomerization of Jaw1 as an intrinsically disordered region (IDR). Importantly, the ectopic expression of an N-terminal region deleted mutant caused the formation of organized smooth ER (OSER), structures such as nuclear karmellae and whorls, in B16F10 cells. Furthermore, this OSER interfered with the localization of the oligomer and interactors such as the type III inositol 1,4,5-triphosphate receptor (IP3R3) and SUN2. In summary, the N-terminal region of Jaw1 inhibits the formation of OSER as an IDR to maintain the homeostatic localization of interactors on the ER membrane.
format	article
author	Takuma Kozono Hiroyuki Sato Wataru Okumura Chifuyu Jogano Miwa Tamura-Nakano Yuki I. Kawamura Jack Rohrer Takashi Tonozuka Atsushi Nishikawa
author_facet	Takuma Kozono Hiroyuki Sato Wataru Okumura Chifuyu Jogano Miwa Tamura-Nakano Yuki I. Kawamura Jack Rohrer Takashi Tonozuka Atsushi Nishikawa
author_sort	Takuma Kozono
title	The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region
title_short	The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region
title_full	The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region
title_fullStr	The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region
title_full_unstemmed	The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region
title_sort	n-terminal region of jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9b0bbb77d4e84230962f6a8b257e98b9
work_keys_str_mv	AT takumakozono thenterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT hiroyukisato thenterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT wataruokumura thenterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT chifuyujogano thenterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT miwatamuranakano thenterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT yukiikawamura thenterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT jackrohrer thenterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT takashitonozuka thenterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT atsushinishikawa thenterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT takumakozono nterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT hiroyukisato nterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT wataruokumura nterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT chifuyujogano nterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT miwatamuranakano nterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT yukiikawamura nterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT jackrohrer nterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT takashitonozuka nterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion AT atsushinishikawa nterminalregionofjaw1hasaroletoinhibittheformationoforganizedsmoothendoplasmicreticulumasanintrinsicallydisorderedregion
_version_	1718391799779688448

The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region

Ejemplares similares