Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region

Abstract Trachoma, the leading infectious cause of blindness, is caused by Chlamydia trachomatis (Ct), a bacterium of the phylum Chlamydiae. Recent investigations revealed the existence of additional families within the phylum Chlamydiae, also termed Chlamydia-like organisms (CLOs). In this study, t...

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Autores principales:	Ehsan Ghasemian, Aleksandra Inic-Kanada, Astrid Collingro, Florian Tagini, Elisabeth Stein, Hadeel Alchalabi, Nadine Schuerer, Darja Keše, Balgesa Elkheir Babiker, Nicole Borel, Gilbert Greub, Talin Barisani-Asenbauer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b173ff4c2f144639dcfd7be29c0a66c
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spelling	oai:doaj.org-article:9b173ff4c2f144639dcfd7be29c0a66c2021-12-02T11:40:25ZDetection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region10.1038/s41598-018-23887-12045-2322https://doaj.org/article/9b173ff4c2f144639dcfd7be29c0a66c2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-23887-1https://doaj.org/toc/2045-2322Abstract Trachoma, the leading infectious cause of blindness, is caused by Chlamydia trachomatis (Ct), a bacterium of the phylum Chlamydiae. Recent investigations revealed the existence of additional families within the phylum Chlamydiae, also termed Chlamydia-like organisms (CLOs). In this study, the frequency of Ct and CLOs was examined in the eyes of healthy Sudanese (control) participants and those with trachoma (case). We tested 96 children (54 cases and 42 controls) and 93 adults (51 cases and 42 controls) using broad-range Chlamydiae and Ct-specific (omcB) real-time PCR. Samples positive by broad-range Chlamydiae testing were subjected to DNA sequencing. Overall Chlamydiae prevalence was 36%. Sequences corresponded to unclassified and classified Chlamydiae. Ct infection rate was significantly higher in children (31.5%) compared to adults (0%) with trachoma (p < 0.0001). In general, 21.5% of adults and 4.2% of children tested positive for CLOs (p = 0.0003). Our findings are consistent with previous investigations describing the central role of Ct in trachoma among children. This is the first study examining human eyes for the presence of CLOs. We found an age-dependent distribution of CLO DNA in human eyes with significantly higher positivity in adults. Further studies are needed to understand the impact of CLOs in trachoma pathogenicity and/or protection.Ehsan GhasemianAleksandra Inic-KanadaAstrid CollingroFlorian TaginiElisabeth SteinHadeel AlchalabiNadine SchuererDarja KešeBalgesa Elkheir BabikerNicole BorelGilbert GreubTalin Barisani-AsenbauerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-16 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ehsan Ghasemian Aleksandra Inic-Kanada Astrid Collingro Florian Tagini Elisabeth Stein Hadeel Alchalabi Nadine Schuerer Darja Keše Balgesa Elkheir Babiker Nicole Borel Gilbert Greub Talin Barisani-Asenbauer Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region
description	Abstract Trachoma, the leading infectious cause of blindness, is caused by Chlamydia trachomatis (Ct), a bacterium of the phylum Chlamydiae. Recent investigations revealed the existence of additional families within the phylum Chlamydiae, also termed Chlamydia-like organisms (CLOs). In this study, the frequency of Ct and CLOs was examined in the eyes of healthy Sudanese (control) participants and those with trachoma (case). We tested 96 children (54 cases and 42 controls) and 93 adults (51 cases and 42 controls) using broad-range Chlamydiae and Ct-specific (omcB) real-time PCR. Samples positive by broad-range Chlamydiae testing were subjected to DNA sequencing. Overall Chlamydiae prevalence was 36%. Sequences corresponded to unclassified and classified Chlamydiae. Ct infection rate was significantly higher in children (31.5%) compared to adults (0%) with trachoma (p < 0.0001). In general, 21.5% of adults and 4.2% of children tested positive for CLOs (p = 0.0003). Our findings are consistent with previous investigations describing the central role of Ct in trachoma among children. This is the first study examining human eyes for the presence of CLOs. We found an age-dependent distribution of CLO DNA in human eyes with significantly higher positivity in adults. Further studies are needed to understand the impact of CLOs in trachoma pathogenicity and/or protection.
format	article
author	Ehsan Ghasemian Aleksandra Inic-Kanada Astrid Collingro Florian Tagini Elisabeth Stein Hadeel Alchalabi Nadine Schuerer Darja Keše Balgesa Elkheir Babiker Nicole Borel Gilbert Greub Talin Barisani-Asenbauer
author_facet	Ehsan Ghasemian Aleksandra Inic-Kanada Astrid Collingro Florian Tagini Elisabeth Stein Hadeel Alchalabi Nadine Schuerer Darja Keše Balgesa Elkheir Babiker Nicole Borel Gilbert Greub Talin Barisani-Asenbauer
author_sort	Ehsan Ghasemian
title	Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region
title_short	Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region
title_full	Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region
title_fullStr	Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region
title_full_unstemmed	Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region
title_sort	detection of chlamydiaceae and chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9b173ff4c2f144639dcfd7be29c0a66c
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Detection of Chlamydiaceae and Chlamydia-like organisms on the ocular surface of children and adults from a trachoma-endemic region

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