Germination and in vitro Propagation of Gundelia tournefontii an Important Medicinal Plant

Medicinal plants are important sources of medical and many other pharmaceutical goods. The conventional propagation scheme is the main means of proliferation and takes a long period for proliferation because of poor germination and also low clonal uniformity. Rapid shoot multiplication of Gundelia t...

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Autores principales: Mohamad Shatnawi, Majid Majdalawi, Wesam Shahrour, Taleb R. Abu-Zahra, Abdel Rahman Al-Tawaha
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Publicado: Polish Society of Ecological Engineering (PTIE) 2022
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Acceso en línea:https://doaj.org/article/9b1aef1fafb6477fb112c0435f4aa91c
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spelling oai:doaj.org-article:9b1aef1fafb6477fb112c0435f4aa91c2021-11-22T11:14:28ZGermination and in vitro Propagation of Gundelia tournefontii an Important Medicinal Plant2719-705010.12912/27197050/143006https://doaj.org/article/9b1aef1fafb6477fb112c0435f4aa91c2022-01-01T00:00:00Zhttp://www.ecoeet.com/Germination-and-in-vitro-Propagation-of-Gundelia-tournefontii-an-Important-Medicinal,143006,0,2.htmlhttps://doaj.org/toc/2719-7050Medicinal plants are important sources of medical and many other pharmaceutical goods. The conventional propagation scheme is the main means of proliferation and takes a long period for proliferation because of poor germination and also low clonal uniformity. Rapid shoot multiplication of Gundelia tournefontii was attained from meristems on Murashige and Skoog (MS) basal medium containing 6-Benzyladenine (BAP) or kinetin, with the adding of 30 g/L sucrose. The highest number of new microshoots per explant (9.2) was attained on MS medium enhanced with BAP and 0.05 mg/L IBA. After 13 to 14 days microhoots start rooted on MS medium enhanced either Indole-3-butyric acid (IBA), Indole -3-acetic acid (IAA), or Naphthalene acetic acid (NAA) with the addition of 30 g/L sucrose. Using 100 to 300 ppm Gibbreline GA3 resulted in an increase germination percentage when soaking with GA3 for 12 hours. Gamma radiation had a significant effect on the growth of the in vitro explants. Gamma radiation had a significant effect on the germination percentage G. tournefontii seeds. The in vitro propagation plant of G. tournefontii plants could be used for the marketable clonal propagation of this important species or for future studiesMohamad ShatnawiMajid MajdalawiWesam ShahrourTaleb R. Abu-ZahraAbdel Rahman Al-TawahaPolish Society of Ecological Engineering (PTIE)articleproliferationmedicinal plantroot formationgibbrelinegundelia tournefontiiEcologyQH540-549.5ENEcological Engineering & Environmental Technology, Vol 23, Iss 1, Pp 57-64 (2022)
institution DOAJ
collection DOAJ
language EN
topic proliferation
medicinal plant
root formation
gibbreline
gundelia tournefontii
Ecology
QH540-549.5
spellingShingle proliferation
medicinal plant
root formation
gibbreline
gundelia tournefontii
Ecology
QH540-549.5
Mohamad Shatnawi
Majid Majdalawi
Wesam Shahrour
Taleb R. Abu-Zahra
Abdel Rahman Al-Tawaha
Germination and in vitro Propagation of Gundelia tournefontii an Important Medicinal Plant
description Medicinal plants are important sources of medical and many other pharmaceutical goods. The conventional propagation scheme is the main means of proliferation and takes a long period for proliferation because of poor germination and also low clonal uniformity. Rapid shoot multiplication of Gundelia tournefontii was attained from meristems on Murashige and Skoog (MS) basal medium containing 6-Benzyladenine (BAP) or kinetin, with the adding of 30 g/L sucrose. The highest number of new microshoots per explant (9.2) was attained on MS medium enhanced with BAP and 0.05 mg/L IBA. After 13 to 14 days microhoots start rooted on MS medium enhanced either Indole-3-butyric acid (IBA), Indole -3-acetic acid (IAA), or Naphthalene acetic acid (NAA) with the addition of 30 g/L sucrose. Using 100 to 300 ppm Gibbreline GA3 resulted in an increase germination percentage when soaking with GA3 for 12 hours. Gamma radiation had a significant effect on the growth of the in vitro explants. Gamma radiation had a significant effect on the germination percentage G. tournefontii seeds. The in vitro propagation plant of G. tournefontii plants could be used for the marketable clonal propagation of this important species or for future studies
format article
author Mohamad Shatnawi
Majid Majdalawi
Wesam Shahrour
Taleb R. Abu-Zahra
Abdel Rahman Al-Tawaha
author_facet Mohamad Shatnawi
Majid Majdalawi
Wesam Shahrour
Taleb R. Abu-Zahra
Abdel Rahman Al-Tawaha
author_sort Mohamad Shatnawi
title Germination and in vitro Propagation of Gundelia tournefontii an Important Medicinal Plant
title_short Germination and in vitro Propagation of Gundelia tournefontii an Important Medicinal Plant
title_full Germination and in vitro Propagation of Gundelia tournefontii an Important Medicinal Plant
title_fullStr Germination and in vitro Propagation of Gundelia tournefontii an Important Medicinal Plant
title_full_unstemmed Germination and in vitro Propagation of Gundelia tournefontii an Important Medicinal Plant
title_sort germination and in vitro propagation of gundelia tournefontii an important medicinal plant
publisher Polish Society of Ecological Engineering (PTIE)
publishDate 2022
url https://doaj.org/article/9b1aef1fafb6477fb112c0435f4aa91c
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