Selective Detection of Nano-Sized Diagnostic Markers Using Au-ZnO Nanorod-Based Surface-Enhanced Raman Spectroscopy (SERS) in Ureteral Obstruction Models

Sanghwa Lee,1,* Jung-Man Namgoong,2,* Miyeon Jue,1 Yujin Joung,1 Chae-Min Ryu,3,4 Dong-Myung Shin,4 Myung-Soo Choo,3 Jun Ki Kim1,5 1Biomedical Engineering Research Center, Asan Medical Center, Seoul 05505, Republic of Korea; 2Department of Surgery, Asan Medical Center, University of Ulsan College of...

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Autores principales:	Lee S, Namgoong JM, Jue M, Joung Y, Ryu CM, Shin DM, Choo MS, Kim JK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	ureteral obstruction renal injury nano-sized biomarker zno nanorods surface-enhanced raman spectroscopy principal component analysis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9b1dfc23f16e4509bf8171c45e896d0a
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spelling	oai:doaj.org-article:9b1dfc23f16e4509bf8171c45e896d0a2021-12-02T11:10:25ZSelective Detection of Nano-Sized Diagnostic Markers Using Au-ZnO Nanorod-Based Surface-Enhanced Raman Spectroscopy (SERS) in Ureteral Obstruction Models1178-2013https://doaj.org/article/9b1dfc23f16e4509bf8171c45e896d0a2020-10-01T00:00:00Zhttps://www.dovepress.com/selective-detection-of-nano-sized-diagnostic-markers-using-au-zno-nano-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sanghwa Lee,1,* Jung-Man Namgoong,2,* Miyeon Jue,1 Yujin Joung,1 Chae-Min Ryu,3,4 Dong-Myung Shin,4 Myung-Soo Choo,3 Jun Ki Kim1,5 1Biomedical Engineering Research Center, Asan Medical Center, Seoul 05505, Republic of Korea; 2Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea; 3Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea; 4Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea; 5Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea*These authors contributed equally to this workCorrespondence: Jun Ki Kim Email kim@amc.seoul.krBackground: This study investigated the diagnosis of renal diseases using a biochip capable of detecting nano-sized biomarkers. Raman measurements from a kidney injury model were taken, and the feasibility of early diagnosis was assessed.Materials and Methods: Rat models with mild and severe unilateral ureteral obstructions were created, with the injury to the kidney varying according to the tightness of the stricture. After generating the animal ureteral obstruction models, urine was collected from the kidney and bladder.Results and Discussion: After confirming the presence of renal injury, urine drops were placed onto a Raman chip whose surface had been enhanced with Au-ZnO nanorods, allowing nano-sized biomarkers that diffused into the nanogaps to be selectively amplified. The Raman signals varied according to the severity of the renal damage, and these differences were statistically confirmed.Conclusion: These results confirm that ureteral stricture causes kidney injury and that signals in the urine from the release of nano-biomarkers can be monitored using surface-enhanced Raman spectroscopy.Keywords: ureteral obstruction, renal injury, nano-sized biomarker, ZnO nanorods, surface-enhanced Raman spectroscopy, principal component analysisLee SNamgoong JMJue MJoung YRyu CMShin DMChoo MSKim JKDove Medical Pressarticleureteral obstructionrenal injurynano-sized biomarkerzno nanorodssurface-enhanced raman spectroscopyprincipal component analysisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 8121-8130 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	ureteral obstruction renal injury nano-sized biomarker zno nanorods surface-enhanced raman spectroscopy principal component analysis Medicine (General) R5-920
spellingShingle	ureteral obstruction renal injury nano-sized biomarker zno nanorods surface-enhanced raman spectroscopy principal component analysis Medicine (General) R5-920 Lee S Namgoong JM Jue M Joung Y Ryu CM Shin DM Choo MS Kim JK Selective Detection of Nano-Sized Diagnostic Markers Using Au-ZnO Nanorod-Based Surface-Enhanced Raman Spectroscopy (SERS) in Ureteral Obstruction Models
description	Sanghwa Lee,1,* Jung-Man Namgoong,2,* Miyeon Jue,1 Yujin Joung,1 Chae-Min Ryu,3,4 Dong-Myung Shin,4 Myung-Soo Choo,3 Jun Ki Kim1,5 1Biomedical Engineering Research Center, Asan Medical Center, Seoul 05505, Republic of Korea; 2Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea; 3Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea; 4Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea; 5Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea*These authors contributed equally to this workCorrespondence: Jun Ki Kim Email kim@amc.seoul.krBackground: This study investigated the diagnosis of renal diseases using a biochip capable of detecting nano-sized biomarkers. Raman measurements from a kidney injury model were taken, and the feasibility of early diagnosis was assessed.Materials and Methods: Rat models with mild and severe unilateral ureteral obstructions were created, with the injury to the kidney varying according to the tightness of the stricture. After generating the animal ureteral obstruction models, urine was collected from the kidney and bladder.Results and Discussion: After confirming the presence of renal injury, urine drops were placed onto a Raman chip whose surface had been enhanced with Au-ZnO nanorods, allowing nano-sized biomarkers that diffused into the nanogaps to be selectively amplified. The Raman signals varied according to the severity of the renal damage, and these differences were statistically confirmed.Conclusion: These results confirm that ureteral stricture causes kidney injury and that signals in the urine from the release of nano-biomarkers can be monitored using surface-enhanced Raman spectroscopy.Keywords: ureteral obstruction, renal injury, nano-sized biomarker, ZnO nanorods, surface-enhanced Raman spectroscopy, principal component analysis
format	article
author	Lee S Namgoong JM Jue M Joung Y Ryu CM Shin DM Choo MS Kim JK
author_facet	Lee S Namgoong JM Jue M Joung Y Ryu CM Shin DM Choo MS Kim JK
author_sort	Lee S
title	Selective Detection of Nano-Sized Diagnostic Markers Using Au-ZnO Nanorod-Based Surface-Enhanced Raman Spectroscopy (SERS) in Ureteral Obstruction Models
title_short	Selective Detection of Nano-Sized Diagnostic Markers Using Au-ZnO Nanorod-Based Surface-Enhanced Raman Spectroscopy (SERS) in Ureteral Obstruction Models
title_full	Selective Detection of Nano-Sized Diagnostic Markers Using Au-ZnO Nanorod-Based Surface-Enhanced Raman Spectroscopy (SERS) in Ureteral Obstruction Models
title_fullStr	Selective Detection of Nano-Sized Diagnostic Markers Using Au-ZnO Nanorod-Based Surface-Enhanced Raman Spectroscopy (SERS) in Ureteral Obstruction Models
title_full_unstemmed	Selective Detection of Nano-Sized Diagnostic Markers Using Au-ZnO Nanorod-Based Surface-Enhanced Raman Spectroscopy (SERS) in Ureteral Obstruction Models
title_sort	selective detection of nano-sized diagnostic markers using au-zno nanorod-based surface-enhanced raman spectroscopy (sers) in ureteral obstruction models
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/9b1dfc23f16e4509bf8171c45e896d0a
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Selective Detection of Nano-Sized Diagnostic Markers Using Au-ZnO Nanorod-Based Surface-Enhanced Raman Spectroscopy (SERS) in Ureteral Obstruction Models

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