Inulin-grown Faecalibacterium prausnitzii cross-feeds fructose to the human intestinal epithelium

Many chronic diseases are associated with decreased abundance of the gut commensal Faecalibacterium prausnitzii. This strict anaerobe can grow on dietary fibers, e.g., prebiotics, and produce high levels of butyrate, often associated to epithelial metabolism and health. However, little is known abou...

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Autores principales:	Raphael R. Fagundes, Arno R. Bourgonje, Ali Saeed, Arnau Vich Vila, Niels Plomp, Tjasso Blokzijl, Mehdi Sadaghian Sadabad, Julius Z. H. von Martels, Sander S. van Leeuwen, Rinse K. Weersma, Gerard Dijkstra, Hermie J. M. Harmsen, Klaas Nico Faber
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	gut bacteria dysbiosis fructose intestinal epithelium faecalibacterium inulin Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/9b241095487e4d15a87bb02ea127b11a
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spelling	oai:doaj.org-article:9b241095487e4d15a87bb02ea127b11a2021-11-26T11:19:48ZInulin-grown Faecalibacterium prausnitzii cross-feeds fructose to the human intestinal epithelium1949-09761949-098410.1080/19490976.2021.1993582https://doaj.org/article/9b241095487e4d15a87bb02ea127b11a2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/19490976.2021.1993582https://doaj.org/toc/1949-0976https://doaj.org/toc/1949-0984Many chronic diseases are associated with decreased abundance of the gut commensal Faecalibacterium prausnitzii. This strict anaerobe can grow on dietary fibers, e.g., prebiotics, and produce high levels of butyrate, often associated to epithelial metabolism and health. However, little is known about other F. prausnitzii metabolites that may affect the colonic epithelium. Here, we analyzed prebiotic cross-feeding between F. prausnitzii and intestinal epithelial (Caco-2) cells in a “Human-oxygen Bacteria-anaerobic” coculture system. Inulin-grown F. prausnitzii enhanced Caco-2 viability and suppressed inflammation- and oxidative stress-marker expression. Inulin-grown F. prausnitzii produced excess butyrate and fructose, but only fructose efficiently promoted Caco-2 growth. Finally, fecal microbial taxonomy analysis (16S sequencing) from healthy volunteers (n = 255) showed the strongest positive correlation for F. prausnitzii abundance and stool fructose levels. We show that fructose, produced and accumulated in a fiber-rich colonic environment, supports colonic epithelium growth, while butyrate does not.Raphael R. FagundesArno R. BourgonjeAli SaeedArnau Vich VilaNiels PlompTjasso BlokzijlMehdi Sadaghian SadabadJulius Z. H. von MartelsSander S. van LeeuwenRinse K. WeersmaGerard DijkstraHermie J. M. HarmsenKlaas Nico FaberTaylor & Francis Grouparticlegut bacteriadysbiosisfructoseintestinal epitheliumfaecalibacteriuminulinDiseases of the digestive system. GastroenterologyRC799-869ENGut Microbes, Vol 13, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gut bacteria dysbiosis fructose intestinal epithelium faecalibacterium inulin Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	gut bacteria dysbiosis fructose intestinal epithelium faecalibacterium inulin Diseases of the digestive system. Gastroenterology RC799-869 Raphael R. Fagundes Arno R. Bourgonje Ali Saeed Arnau Vich Vila Niels Plomp Tjasso Blokzijl Mehdi Sadaghian Sadabad Julius Z. H. von Martels Sander S. van Leeuwen Rinse K. Weersma Gerard Dijkstra Hermie J. M. Harmsen Klaas Nico Faber Inulin-grown Faecalibacterium prausnitzii cross-feeds fructose to the human intestinal epithelium
description	Many chronic diseases are associated with decreased abundance of the gut commensal Faecalibacterium prausnitzii. This strict anaerobe can grow on dietary fibers, e.g., prebiotics, and produce high levels of butyrate, often associated to epithelial metabolism and health. However, little is known about other F. prausnitzii metabolites that may affect the colonic epithelium. Here, we analyzed prebiotic cross-feeding between F. prausnitzii and intestinal epithelial (Caco-2) cells in a “Human-oxygen Bacteria-anaerobic” coculture system. Inulin-grown F. prausnitzii enhanced Caco-2 viability and suppressed inflammation- and oxidative stress-marker expression. Inulin-grown F. prausnitzii produced excess butyrate and fructose, but only fructose efficiently promoted Caco-2 growth. Finally, fecal microbial taxonomy analysis (16S sequencing) from healthy volunteers (n = 255) showed the strongest positive correlation for F. prausnitzii abundance and stool fructose levels. We show that fructose, produced and accumulated in a fiber-rich colonic environment, supports colonic epithelium growth, while butyrate does not.
format	article
author	Raphael R. Fagundes Arno R. Bourgonje Ali Saeed Arnau Vich Vila Niels Plomp Tjasso Blokzijl Mehdi Sadaghian Sadabad Julius Z. H. von Martels Sander S. van Leeuwen Rinse K. Weersma Gerard Dijkstra Hermie J. M. Harmsen Klaas Nico Faber
author_facet	Raphael R. Fagundes Arno R. Bourgonje Ali Saeed Arnau Vich Vila Niels Plomp Tjasso Blokzijl Mehdi Sadaghian Sadabad Julius Z. H. von Martels Sander S. van Leeuwen Rinse K. Weersma Gerard Dijkstra Hermie J. M. Harmsen Klaas Nico Faber
author_sort	Raphael R. Fagundes
title	Inulin-grown Faecalibacterium prausnitzii cross-feeds fructose to the human intestinal epithelium
title_short	Inulin-grown Faecalibacterium prausnitzii cross-feeds fructose to the human intestinal epithelium
title_full	Inulin-grown Faecalibacterium prausnitzii cross-feeds fructose to the human intestinal epithelium
title_fullStr	Inulin-grown Faecalibacterium prausnitzii cross-feeds fructose to the human intestinal epithelium
title_full_unstemmed	Inulin-grown Faecalibacterium prausnitzii cross-feeds fructose to the human intestinal epithelium
title_sort	inulin-grown faecalibacterium prausnitzii cross-feeds fructose to the human intestinal epithelium
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/9b241095487e4d15a87bb02ea127b11a
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Inulin-grown Faecalibacterium prausnitzii cross-feeds fructose to the human intestinal epithelium

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