Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis

Abstract Macroalgae host various symbionts on their surface, which play a critical role in their growth and development processes. However, there is still incomplete understanding of this epiphytic bacteria-host algae interactions. This study comprehensively analysed variation of the epiphytic bacte...

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Autores principales:	Pengbing Pei, Muhammad Aslam, Hong Du, Honghao Liang, Hui Wang, Xiaojuan Liu, Weizhou Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b24fc93519e4c27ae21f1c56f5c75ae
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spelling	oai:doaj.org-article:9b24fc93519e4c27ae21f1c56f5c75ae2021-12-02T16:45:11ZEnvironmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis10.1038/s41598-021-87977-32045-2322https://doaj.org/article/9b24fc93519e4c27ae21f1c56f5c75ae2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87977-3https://doaj.org/toc/2045-2322Abstract Macroalgae host various symbionts on their surface, which play a critical role in their growth and development processes. However, there is still incomplete understanding of this epiphytic bacteria-host algae interactions. This study comprehensively analysed variation of the epiphytic bacterial communities (EBC) composition of red macroalga Gracilariopsis lemaneiformis at different geographic locations and environmental factors (i.e., nitrogen and phosphorus), which shape the EBC composition of G. lemaneiformis. The composition and structure of EBC were characterized using high throughput sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. The results revealed that epiphytic bacteria varied significantly among three different geographic locations in China, i.e., Nan’ao Island (NA), Lianjiang County (LJ), and Nanri Island (NR). Redundancy analysis (RDA) showed that the relative abundance of Bacteroidetes, Firmicutes, Verrucomicrobia, and Epsilonbacteraeota at NR were strongly positively correlated with total nitrogen (TN), total phosphorus (TP), nitrate nitrogen (NO3-N), and dissolved inorganic nitrogen (DIN), but negatively correlated with nitrite nitrogen (NO2-N). The relative abundance of Cyanobacteria at NA and LJ were strongly positively correlated with NO2-N, but negatively correlated with TN, TP, NO3-N, and DIN. Besides, the Mantel test results indicated that the EBC composition was significantly correlated with these environmental factors, which was also confirmed by Spearman correlation analysis. Thus, environmental factors such as NO3-N and DIN play a key role in the community composition of epiphytic bacteria on G. lemaneiformis. This study provides important baseline knowledge on the community composition of epiphytic bacteria on G. lemaneiformis and shows correlation between different epiphytic bacteria and their surrounding environmental factors.Pengbing PeiMuhammad AslamHong DuHonghao LiangHui WangXiaojuan LiuWeizhou ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pengbing Pei Muhammad Aslam Hong Du Honghao Liang Hui Wang Xiaojuan Liu Weizhou Chen Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis
description	Abstract Macroalgae host various symbionts on their surface, which play a critical role in their growth and development processes. However, there is still incomplete understanding of this epiphytic bacteria-host algae interactions. This study comprehensively analysed variation of the epiphytic bacterial communities (EBC) composition of red macroalga Gracilariopsis lemaneiformis at different geographic locations and environmental factors (i.e., nitrogen and phosphorus), which shape the EBC composition of G. lemaneiformis. The composition and structure of EBC were characterized using high throughput sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. The results revealed that epiphytic bacteria varied significantly among three different geographic locations in China, i.e., Nan’ao Island (NA), Lianjiang County (LJ), and Nanri Island (NR). Redundancy analysis (RDA) showed that the relative abundance of Bacteroidetes, Firmicutes, Verrucomicrobia, and Epsilonbacteraeota at NR were strongly positively correlated with total nitrogen (TN), total phosphorus (TP), nitrate nitrogen (NO3-N), and dissolved inorganic nitrogen (DIN), but negatively correlated with nitrite nitrogen (NO2-N). The relative abundance of Cyanobacteria at NA and LJ were strongly positively correlated with NO2-N, but negatively correlated with TN, TP, NO3-N, and DIN. Besides, the Mantel test results indicated that the EBC composition was significantly correlated with these environmental factors, which was also confirmed by Spearman correlation analysis. Thus, environmental factors such as NO3-N and DIN play a key role in the community composition of epiphytic bacteria on G. lemaneiformis. This study provides important baseline knowledge on the community composition of epiphytic bacteria on G. lemaneiformis and shows correlation between different epiphytic bacteria and their surrounding environmental factors.
format	article
author	Pengbing Pei Muhammad Aslam Hong Du Honghao Liang Hui Wang Xiaojuan Liu Weizhou Chen
author_facet	Pengbing Pei Muhammad Aslam Hong Du Honghao Liang Hui Wang Xiaojuan Liu Weizhou Chen
author_sort	Pengbing Pei
title	Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis
title_short	Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis
title_full	Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis
title_fullStr	Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis
title_full_unstemmed	Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis
title_sort	environmental factors shape the epiphytic bacterial communities of gracilariopsis lemaneiformis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9b24fc93519e4c27ae21f1c56f5c75ae
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Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis

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