Biological and Chemical Adaptation to Endogenous Hydrogen Peroxide Production in <named-content content-type="genus-species">Streptococcus pneumoniae</named-content> D39

ABSTRACT The catalase-negative, facultative anaerobe Streptococcus pneumoniae D39 is naturally resistant to hydrogen peroxide (H2O2) produced endogenously by pyruvate oxidase (SpxB). Here, we investigate the adaptive response to endogenously produced H2O2. We show that lactate oxidase, which convert...

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Autores principales:	John P. Lisher, Ho-Ching Tiffany Tsui, Smirla Ramos-Montañez, Kristy L. Hentchel, Julia E. Martin, Jonathan C. Trinidad, Malcolm E. Winkler, David P. Giedroc
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Streptococcus pneumoniae hydrogen peroxide stress pyruvate oxidase sulfenylation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9b263744cdb6477bb91a837cfbe40d8f
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spelling	oai:doaj.org-article:9b263744cdb6477bb91a837cfbe40d8f2021-11-15T15:22:03ZBiological and Chemical Adaptation to Endogenous Hydrogen Peroxide Production in <named-content content-type="genus-species">Streptococcus pneumoniae</named-content> D3910.1128/mSphere.00291-162379-5042https://doaj.org/article/9b263744cdb6477bb91a837cfbe40d8f2017-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00291-16https://doaj.org/toc/2379-5042ABSTRACT The catalase-negative, facultative anaerobe Streptococcus pneumoniae D39 is naturally resistant to hydrogen peroxide (H2O2) produced endogenously by pyruvate oxidase (SpxB). Here, we investigate the adaptive response to endogenously produced H2O2. We show that lactate oxidase, which converts lactate to pyruvate, positively impacts pyruvate flux through SpxB and that ΔlctO mutants produce significantly lower H2O2. In addition, both the SpxB pathway and a candidate pyruvate dehydrogenase complex (PDHC) pathway contribute to acetyl coenzyme A (acetyl-CoA) production during aerobic growth, and the pyruvate format lyase (PFL) pathway is the major acetyl-CoA pathway during anaerobic growth. Microarray analysis of the D39 strain cultured under aerobic versus strict anaerobic conditions shows upregulation of spxB, a gene encoding a rhodanese-like protein (locus tag spd0091), tpxD, sodA, piuB, piuD, and an Fe-S protein biogenesis operon under H2O2-producing conditions. Proteome profiling of H2O2-induced sulfenylation reveals that sulfenylation levels correlate with cellular H2O2 production, with endogenous sulfenylation of ≈50 proteins. Deletion of tpxD increases cellular sulfenylation 5-fold and has an inhibitory effect on ATP generation. Two major targets of protein sulfenylation are glyceraldehyde-3-phosphate dehydrogenase (GapA) and SpxB itself, but targets also include pyruvate kinase, LctO, AdhE, and acetate kinase (AckA). Sulfenylation of GapA is inhibitory, while the effect on SpxB activity is negligible. Strikingly, four enzymes of capsular polysaccharide biosynthesis are sulfenylated, as are enzymes associated with nucleotide biosynthesis via ribulose-5-phosphate. We propose that LctO/SpxB-generated H2O2 functions as a signaling molecule to downregulate capsule production and drive altered flux through sugar utilization pathways. IMPORTANCE Adaptation to endogenous oxidative stress is an integral aspect of Streptococcus pneumoniae colonization and virulence. In this work, we identify key transcriptomic and proteomic features of the pneumococcal endogenous oxidative stress response. The thiol peroxidase TpxD plays a critical role in adaptation to endogenous H2O2 and serves to limit protein sulfenylation of glycolytic, capsule, and nucleotide biosynthesis enzymes in S. pneumoniae.John P. LisherHo-Ching Tiffany TsuiSmirla Ramos-MontañezKristy L. HentchelJulia E. MartinJonathan C. TrinidadMalcolm E. WinklerDavid P. GiedrocAmerican Society for MicrobiologyarticleStreptococcus pneumoniaehydrogen peroxide stresspyruvate oxidasesulfenylationMicrobiologyQR1-502ENmSphere, Vol 2, Iss 1 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Streptococcus pneumoniae hydrogen peroxide stress pyruvate oxidase sulfenylation Microbiology QR1-502
spellingShingle	Streptococcus pneumoniae hydrogen peroxide stress pyruvate oxidase sulfenylation Microbiology QR1-502 John P. Lisher Ho-Ching Tiffany Tsui Smirla Ramos-Montañez Kristy L. Hentchel Julia E. Martin Jonathan C. Trinidad Malcolm E. Winkler David P. Giedroc Biological and Chemical Adaptation to Endogenous Hydrogen Peroxide Production in <named-content content-type="genus-species">Streptococcus pneumoniae</named-content> D39
description	ABSTRACT The catalase-negative, facultative anaerobe Streptococcus pneumoniae D39 is naturally resistant to hydrogen peroxide (H2O2) produced endogenously by pyruvate oxidase (SpxB). Here, we investigate the adaptive response to endogenously produced H2O2. We show that lactate oxidase, which converts lactate to pyruvate, positively impacts pyruvate flux through SpxB and that ΔlctO mutants produce significantly lower H2O2. In addition, both the SpxB pathway and a candidate pyruvate dehydrogenase complex (PDHC) pathway contribute to acetyl coenzyme A (acetyl-CoA) production during aerobic growth, and the pyruvate format lyase (PFL) pathway is the major acetyl-CoA pathway during anaerobic growth. Microarray analysis of the D39 strain cultured under aerobic versus strict anaerobic conditions shows upregulation of spxB, a gene encoding a rhodanese-like protein (locus tag spd0091), tpxD, sodA, piuB, piuD, and an Fe-S protein biogenesis operon under H2O2-producing conditions. Proteome profiling of H2O2-induced sulfenylation reveals that sulfenylation levels correlate with cellular H2O2 production, with endogenous sulfenylation of ≈50 proteins. Deletion of tpxD increases cellular sulfenylation 5-fold and has an inhibitory effect on ATP generation. Two major targets of protein sulfenylation are glyceraldehyde-3-phosphate dehydrogenase (GapA) and SpxB itself, but targets also include pyruvate kinase, LctO, AdhE, and acetate kinase (AckA). Sulfenylation of GapA is inhibitory, while the effect on SpxB activity is negligible. Strikingly, four enzymes of capsular polysaccharide biosynthesis are sulfenylated, as are enzymes associated with nucleotide biosynthesis via ribulose-5-phosphate. We propose that LctO/SpxB-generated H2O2 functions as a signaling molecule to downregulate capsule production and drive altered flux through sugar utilization pathways. IMPORTANCE Adaptation to endogenous oxidative stress is an integral aspect of Streptococcus pneumoniae colonization and virulence. In this work, we identify key transcriptomic and proteomic features of the pneumococcal endogenous oxidative stress response. The thiol peroxidase TpxD plays a critical role in adaptation to endogenous H2O2 and serves to limit protein sulfenylation of glycolytic, capsule, and nucleotide biosynthesis enzymes in S. pneumoniae.
format	article
author	John P. Lisher Ho-Ching Tiffany Tsui Smirla Ramos-Montañez Kristy L. Hentchel Julia E. Martin Jonathan C. Trinidad Malcolm E. Winkler David P. Giedroc
author_facet	John P. Lisher Ho-Ching Tiffany Tsui Smirla Ramos-Montañez Kristy L. Hentchel Julia E. Martin Jonathan C. Trinidad Malcolm E. Winkler David P. Giedroc
author_sort	John P. Lisher
title	Biological and Chemical Adaptation to Endogenous Hydrogen Peroxide Production in <named-content content-type="genus-species">Streptococcus pneumoniae</named-content> D39
title_short	Biological and Chemical Adaptation to Endogenous Hydrogen Peroxide Production in <named-content content-type="genus-species">Streptococcus pneumoniae</named-content> D39
title_full	Biological and Chemical Adaptation to Endogenous Hydrogen Peroxide Production in <named-content content-type="genus-species">Streptococcus pneumoniae</named-content> D39
title_fullStr	Biological and Chemical Adaptation to Endogenous Hydrogen Peroxide Production in <named-content content-type="genus-species">Streptococcus pneumoniae</named-content> D39
title_full_unstemmed	Biological and Chemical Adaptation to Endogenous Hydrogen Peroxide Production in <named-content content-type="genus-species">Streptococcus pneumoniae</named-content> D39
title_sort	biological and chemical adaptation to endogenous hydrogen peroxide production in <named-content content-type="genus-species">streptococcus pneumoniae</named-content> d39
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/9b263744cdb6477bb91a837cfbe40d8f
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Biological and Chemical Adaptation to Endogenous Hydrogen Peroxide Production in <named-content content-type="genus-species">Streptococcus pneumoniae</named-content> D39

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