Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system

Abstract Amyloid plaques are small (~ 50 μm), highly-dense aggregates of amyloid beta (Aβ) protein in brain tissue, supposed to play a key role in pathogenesis of Alzheimer’s disease (AD). Plaques´ in vivo detection, spatial distribution and quantitative characterization could be an essential marker...

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Autores principales:	Michaela Kavkova, Tomas Zikmund, Annu Kala, Jakub Salplachta, Stephanie L. Proskauer Pena, Josef Kaiser, Karel Jezek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b2736a730b74424bb8554ef8825cea9
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spelling	oai:doaj.org-article:9b2736a730b74424bb8554ef8825cea92021-12-02T13:17:55ZContrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system10.1038/s41598-021-84579-x2045-2322https://doaj.org/article/9b2736a730b74424bb8554ef8825cea92021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84579-xhttps://doaj.org/toc/2045-2322Abstract Amyloid plaques are small (~ 50 μm), highly-dense aggregates of amyloid beta (Aβ) protein in brain tissue, supposed to play a key role in pathogenesis of Alzheimer’s disease (AD). Plaques´ in vivo detection, spatial distribution and quantitative characterization could be an essential marker in diagnostics and evaluation of AD progress. However, current imaging methods in clinics possess substantial limits in sensitivity towards Aβ plaques to play a considerable role in AD screening. Contrast enhanced X-ray micro computed tomography (micro CT) is an emerging highly sensitive imaging technique capable of high resolution visualization of rodent brain. In this study we show the absorption based contrast enhanced X-ray micro CT imaging is viable method for detection and 3D analysis of Aβ plaques in transgenic rodent models of Alzheimer’s disease. Using iodine contrasted brain tissue isolated from the Tg-F344-AD rat model we show the micro CT imaging is capable of precise imaging of Aβ plaques, making possible to further analyze various aspects of their 3D spatial distribution and other properties.Michaela KavkovaTomas ZikmundAnnu KalaJakub SalplachtaStephanie L. Proskauer PenaJosef KaiserKarel JezekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michaela Kavkova Tomas Zikmund Annu Kala Jakub Salplachta Stephanie L. Proskauer Pena Josef Kaiser Karel Jezek Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system
description	Abstract Amyloid plaques are small (~ 50 μm), highly-dense aggregates of amyloid beta (Aβ) protein in brain tissue, supposed to play a key role in pathogenesis of Alzheimer’s disease (AD). Plaques´ in vivo detection, spatial distribution and quantitative characterization could be an essential marker in diagnostics and evaluation of AD progress. However, current imaging methods in clinics possess substantial limits in sensitivity towards Aβ plaques to play a considerable role in AD screening. Contrast enhanced X-ray micro computed tomography (micro CT) is an emerging highly sensitive imaging technique capable of high resolution visualization of rodent brain. In this study we show the absorption based contrast enhanced X-ray micro CT imaging is viable method for detection and 3D analysis of Aβ plaques in transgenic rodent models of Alzheimer’s disease. Using iodine contrasted brain tissue isolated from the Tg-F344-AD rat model we show the micro CT imaging is capable of precise imaging of Aβ plaques, making possible to further analyze various aspects of their 3D spatial distribution and other properties.
format	article
author	Michaela Kavkova Tomas Zikmund Annu Kala Jakub Salplachta Stephanie L. Proskauer Pena Josef Kaiser Karel Jezek
author_facet	Michaela Kavkova Tomas Zikmund Annu Kala Jakub Salplachta Stephanie L. Proskauer Pena Josef Kaiser Karel Jezek
author_sort	Michaela Kavkova
title	Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system
title_short	Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system
title_full	Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system
title_fullStr	Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system
title_full_unstemmed	Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system
title_sort	contrast enhanced x-ray computed tomography imaging of amyloid plaques in alzheimer disease rat model on lab based micro ct system
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9b2736a730b74424bb8554ef8825cea9
work_keys_str_mv	AT michaelakavkova contrastenhancedxraycomputedtomographyimagingofamyloidplaquesinalzheimerdiseaseratmodelonlabbasedmicroctsystem AT tomaszikmund contrastenhancedxraycomputedtomographyimagingofamyloidplaquesinalzheimerdiseaseratmodelonlabbasedmicroctsystem AT annukala contrastenhancedxraycomputedtomographyimagingofamyloidplaquesinalzheimerdiseaseratmodelonlabbasedmicroctsystem AT jakubsalplachta contrastenhancedxraycomputedtomographyimagingofamyloidplaquesinalzheimerdiseaseratmodelonlabbasedmicroctsystem AT stephanielproskauerpena contrastenhancedxraycomputedtomographyimagingofamyloidplaquesinalzheimerdiseaseratmodelonlabbasedmicroctsystem AT josefkaiser contrastenhancedxraycomputedtomographyimagingofamyloidplaquesinalzheimerdiseaseratmodelonlabbasedmicroctsystem AT kareljezek contrastenhancedxraycomputedtomographyimagingofamyloidplaquesinalzheimerdiseaseratmodelonlabbasedmicroctsystem
_version_	1718393347108765696

Contrast enhanced X-ray computed tomography imaging of amyloid plaques in Alzheimer disease rat model on lab based micro CT system

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