Ginger Extract Increases GLUT-4 Expression Preferentially Through AMPK Than PI3K Signalling Pathways in C2C12 Muscle Cells

Marjan Tajik Kord,1 Fatemeh Pourrajab,1,2 Seyedhossein Hekmatimoghaddam3,4 1Department of Biochemistry and Molecular Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Nutrition and Food Security Research Center, Shahid Sadoughi University of Medical Sciences,...

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Autores principales:	Tajik Kord M, Pourrajab F, Hekmatimoghaddam S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	glucose metabolism myoblasts signal transduction. Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/9b2d03d652f44857a19de207e2c25190
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spelling	oai:doaj.org-article:9b2d03d652f44857a19de207e2c251902021-12-02T10:19:19ZGinger Extract Increases GLUT-4 Expression Preferentially Through AMPK Than PI3K Signalling Pathways in C2C12 Muscle Cells1178-7007https://doaj.org/article/9b2d03d652f44857a19de207e2c251902020-09-01T00:00:00Zhttps://www.dovepress.com/ginger-extract-increases-glut-4-expression-preferentially-through-ampk-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Marjan Tajik Kord,1 Fatemeh Pourrajab,1,2 Seyedhossein Hekmatimoghaddam3,4 1Department of Biochemistry and Molecular Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Nutrition and Food Security Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Yazd Cardiovascular Research Center, Afshar Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 4Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranCorrespondence: Seyedhossein Hekmatimoghaddam Tel + 0098-9133518314Fax + 0098-3517256458Email shhekmati2002@yahoo.comPurpose: There are two signal transduction pathways related to glucose metabolism in C2C12 mouse myoblast cells; one through AMP-activated protein kinase (AMPK), and the other through phosphoinositide 3-kinase (PI3K). Ginger is reported to have hypoglycemic effects. The aim of this study was to determine the exact mechanism of action of ginger in those pathways.Methods: C2C12 cells were seeded to four separate experimental groups; Control: treated with 50 μg/mL DMSO in the absence of any inhibitor; Treatment 1: treated with 50 μg/mL ethyl acetate ginger extract without any inhibitor; Treatment 2: treated with 50 μg/mL extract in the presence of 20 μM AMPK inhibitor; Treatment 3: treated with 50 μg/mL extract in the presence of 25 μM PI3K inhibitor. The amount of GLUT-4 protein (an important glucose transporter) was determined in cytosolic and membrane fractions using sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blotting.Results: GLUT-4 concentration was significantly higher in the membrane fraction of cells treated with ethyl acetate ginger extract in the absence of any inhibitor in comparison with cells treated with this extract in the presence of each of the inhibitors (P-value < 0.05). GLUT-4 quantity in the membrane fractions in all groups was more than cytosolic fractions. The amount of GLUT-4 in membrane fraction of treated cells in the presence of PI3K inhibitor was higher than in the cells treated with this extract in the presence of AMPK inhibitor (P-value < 0.05).Conclusion: Ethyl acetate ginger extract affects the amount of GLUT-4 protein in membrane and cytosolic fractions of C2C12 myoblast cells mostly through AMPK pathway but less via PI3K.Keywords: glucose, metabolism, myoblasts, signal transductionTajik Kord MPourrajab FHekmatimoghaddam SDove Medical Pressarticleglucosemetabolismmyoblastssignal transduction.Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 3231-3238 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	glucose metabolism myoblasts signal transduction. Specialties of internal medicine RC581-951
spellingShingle	glucose metabolism myoblasts signal transduction. Specialties of internal medicine RC581-951 Tajik Kord M Pourrajab F Hekmatimoghaddam S Ginger Extract Increases GLUT-4 Expression Preferentially Through AMPK Than PI3K Signalling Pathways in C2C12 Muscle Cells
description	Marjan Tajik Kord,1 Fatemeh Pourrajab,1,2 Seyedhossein Hekmatimoghaddam3,4 1Department of Biochemistry and Molecular Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 2Nutrition and Food Security Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Yazd Cardiovascular Research Center, Afshar Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 4Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranCorrespondence: Seyedhossein Hekmatimoghaddam Tel + 0098-9133518314Fax + 0098-3517256458Email shhekmati2002@yahoo.comPurpose: There are two signal transduction pathways related to glucose metabolism in C2C12 mouse myoblast cells; one through AMP-activated protein kinase (AMPK), and the other through phosphoinositide 3-kinase (PI3K). Ginger is reported to have hypoglycemic effects. The aim of this study was to determine the exact mechanism of action of ginger in those pathways.Methods: C2C12 cells were seeded to four separate experimental groups; Control: treated with 50 μg/mL DMSO in the absence of any inhibitor; Treatment 1: treated with 50 μg/mL ethyl acetate ginger extract without any inhibitor; Treatment 2: treated with 50 μg/mL extract in the presence of 20 μM AMPK inhibitor; Treatment 3: treated with 50 μg/mL extract in the presence of 25 μM PI3K inhibitor. The amount of GLUT-4 protein (an important glucose transporter) was determined in cytosolic and membrane fractions using sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blotting.Results: GLUT-4 concentration was significantly higher in the membrane fraction of cells treated with ethyl acetate ginger extract in the absence of any inhibitor in comparison with cells treated with this extract in the presence of each of the inhibitors (P-value < 0.05). GLUT-4 quantity in the membrane fractions in all groups was more than cytosolic fractions. The amount of GLUT-4 in membrane fraction of treated cells in the presence of PI3K inhibitor was higher than in the cells treated with this extract in the presence of AMPK inhibitor (P-value < 0.05).Conclusion: Ethyl acetate ginger extract affects the amount of GLUT-4 protein in membrane and cytosolic fractions of C2C12 myoblast cells mostly through AMPK pathway but less via PI3K.Keywords: glucose, metabolism, myoblasts, signal transduction
format	article
author	Tajik Kord M Pourrajab F Hekmatimoghaddam S
author_facet	Tajik Kord M Pourrajab F Hekmatimoghaddam S
author_sort	Tajik Kord M
title	Ginger Extract Increases GLUT-4 Expression Preferentially Through AMPK Than PI3K Signalling Pathways in C2C12 Muscle Cells
title_short	Ginger Extract Increases GLUT-4 Expression Preferentially Through AMPK Than PI3K Signalling Pathways in C2C12 Muscle Cells
title_full	Ginger Extract Increases GLUT-4 Expression Preferentially Through AMPK Than PI3K Signalling Pathways in C2C12 Muscle Cells
title_fullStr	Ginger Extract Increases GLUT-4 Expression Preferentially Through AMPK Than PI3K Signalling Pathways in C2C12 Muscle Cells
title_full_unstemmed	Ginger Extract Increases GLUT-4 Expression Preferentially Through AMPK Than PI3K Signalling Pathways in C2C12 Muscle Cells
title_sort	ginger extract increases glut-4 expression preferentially through ampk than pi3k signalling pathways in c2c12 muscle cells
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/9b2d03d652f44857a19de207e2c25190
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Ginger Extract Increases GLUT-4 Expression Preferentially Through AMPK Than PI3K Signalling Pathways in C2C12 Muscle Cells

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