Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica

Abstract Diffusion functional magnetic resonance imaging (DfMRI) has been proposed as a method for functional neuroimaging studies, as an alternative to blood oxygenation level dependent (BOLD)-fMRI. DfMRI is thought to more directly reflect neural activation, but its exact mechanism remains unclear...

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Autores principales:	Yoshifumi Abe, Khieu Van Nguyen, Tomokazu Tsurugizawa, Luisa Ciobanu, Denis Le Bihan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b2f08f242504512a5a23ebd43d8f68d
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spelling	oai:doaj.org-article:9b2f08f242504512a5a23ebd43d8f68d2021-12-02T16:07:01ZModulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica10.1038/s41598-017-05586-52045-2322https://doaj.org/article/9b2f08f242504512a5a23ebd43d8f68d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05586-5https://doaj.org/toc/2045-2322Abstract Diffusion functional magnetic resonance imaging (DfMRI) has been proposed as a method for functional neuroimaging studies, as an alternative to blood oxygenation level dependent (BOLD)-fMRI. DfMRI is thought to more directly reflect neural activation, but its exact mechanism remains unclear. It has been hypothesized that the water apparent diffusion coefficient (ADC) decrease observed upon neural activation results from swelling of neurons or neuron parts. To elucidate the origin of the DfMRI response at cellular level we performed diffusion MR microscopy at 17.2 T in Aplysia californica buccal ganglia and compared the water ADCs at cellular and ganglia levels before and after neuronal activation induced by perfusion with a solution containing dopamine. Neural cell swelling, evidenced from optical microscopy imaging, resulted in an intracellular ADC increase and an ADC decrease at ganglia level. Furthermore, the intracellular ADC increase was found to have a significant positive correlation with the increase in cell size. Our results strongly support the hypothesis that the ADC decrease observed with DfMRI upon neuronal activation at tissue level reflects activation-induced neural cell swelling.Yoshifumi AbeKhieu Van NguyenTomokazu TsurugizawaLuisa CiobanuDenis Le BihanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoshifumi Abe Khieu Van Nguyen Tomokazu Tsurugizawa Luisa Ciobanu Denis Le Bihan Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica
description	Abstract Diffusion functional magnetic resonance imaging (DfMRI) has been proposed as a method for functional neuroimaging studies, as an alternative to blood oxygenation level dependent (BOLD)-fMRI. DfMRI is thought to more directly reflect neural activation, but its exact mechanism remains unclear. It has been hypothesized that the water apparent diffusion coefficient (ADC) decrease observed upon neural activation results from swelling of neurons or neuron parts. To elucidate the origin of the DfMRI response at cellular level we performed diffusion MR microscopy at 17.2 T in Aplysia californica buccal ganglia and compared the water ADCs at cellular and ganglia levels before and after neuronal activation induced by perfusion with a solution containing dopamine. Neural cell swelling, evidenced from optical microscopy imaging, resulted in an intracellular ADC increase and an ADC decrease at ganglia level. Furthermore, the intracellular ADC increase was found to have a significant positive correlation with the increase in cell size. Our results strongly support the hypothesis that the ADC decrease observed with DfMRI upon neuronal activation at tissue level reflects activation-induced neural cell swelling.
format	article
author	Yoshifumi Abe Khieu Van Nguyen Tomokazu Tsurugizawa Luisa Ciobanu Denis Le Bihan
author_facet	Yoshifumi Abe Khieu Van Nguyen Tomokazu Tsurugizawa Luisa Ciobanu Denis Le Bihan
author_sort	Yoshifumi Abe
title	Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica
title_short	Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica
title_full	Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica
title_fullStr	Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica
title_full_unstemmed	Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica
title_sort	modulation of water diffusion by activation-induced neural cell swelling in aplysia californica
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9b2f08f242504512a5a23ebd43d8f68d
work_keys_str_mv	AT yoshifumiabe modulationofwaterdiffusionbyactivationinducedneuralcellswellinginaplysiacalifornica AT khieuvannguyen modulationofwaterdiffusionbyactivationinducedneuralcellswellinginaplysiacalifornica AT tomokazutsurugizawa modulationofwaterdiffusionbyactivationinducedneuralcellswellinginaplysiacalifornica AT luisaciobanu modulationofwaterdiffusionbyactivationinducedneuralcellswellinginaplysiacalifornica AT denislebihan modulationofwaterdiffusionbyactivationinducedneuralcellswellinginaplysiacalifornica
_version_	1718384792322441216

Modulation of water diffusion by activation-induced neural cell swelling in Aplysia Californica

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