Field evaluation of two rapid diagnostic tests for Neisseria meningitidis serogroup A during the 2006 outbreak in Niger.

The Pastorex((R)) (BioRad) rapid agglutination test is one of the main rapid diagnostic tests (RDTs) for meningococcal disease currently in use in the "meningitis belt". Earlier evaluations, performed after heating and centrifugation of cerebrospinal fluid (CSF) samples, under good laborat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Angela M C Rose, Sibylle Gerstl, Ali E-H Mahamane, Fati Sidikou, Saacou Djibo, Laurence Bonte, Dominique A Caugant, Philippe J Guerin, Suzanne Chanteau
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b3d839bac224b5fa097b3a230a5b23f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9b3d839bac224b5fa097b3a230a5b23f
record_format	dspace
spelling	oai:doaj.org-article:9b3d839bac224b5fa097b3a230a5b23f2021-11-25T06:19:56ZField evaluation of two rapid diagnostic tests for Neisseria meningitidis serogroup A during the 2006 outbreak in Niger.1932-620310.1371/journal.pone.0007326https://doaj.org/article/9b3d839bac224b5fa097b3a230a5b23f2009-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19802392/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The Pastorex((R)) (BioRad) rapid agglutination test is one of the main rapid diagnostic tests (RDTs) for meningococcal disease currently in use in the "meningitis belt". Earlier evaluations, performed after heating and centrifugation of cerebrospinal fluid (CSF) samples, under good laboratory conditions, showed high sensitivity and specificity. However, during an epidemic, the test may be used without prior sample preparation. Recently a new, easy-to-use dipstick RDT for meningococcal disease detection on CSF was developed by the Centre de Recherche Médicale et Sanitaire in Niger and the Pasteur Institute in France. We estimate diagnostic accuracy in the field during the 2006 outbreak of Neisseria meningitidis serogroup A in Maradi, Niger, for the dipstick RDT and Pastorex((R)) on unprepared CSF, (a) by comparing each test's sensitivity and specificity with previously reported values; and (b) by comparing results for each test on paired samples, using McNemar's test. We also (c) estimate diagnostic accuracy of the dipstick RDT on diluted whole blood. We tested unprepared CSF and diluted whole blood from 126 patients with suspected meningococcal disease presenting at four health posts. (a) Pastorex((R)) sensitivity (69%; 95%CI 57-79) was significantly lower than found previously for prepared CSF samples [87% (81-91); or 88% (85-91)], as was specificity [81% (95%CI 68-91) vs 93% (90-95); or 93% (87-96)]. Sensitivity of the dipstick RDT [89% (95%CI 80-95)] was similar to previously reported values for ideal laboratory conditions [89% (84-93) and 94% (90-96)]. Specificity, at 62% (95%CI 48-75), was significantly lower than found previously [94% (92-96) and 97% (94-99)]. (b) McNemar's test for the dipstick RDT vs Pastorex((R)) was statistically significant (p<0.001). (c) The dipstick RDT did not perform satisfactorily on diluted whole blood (sensitivity 73%; specificity 57%).Sensitivity and specificity of Pastorex((R)) without prior CSF preparation were poorer than previously reported results from prepared samples; therefore we caution against using this test during an epidemic if sample preparation is not possible. For the dipstick RDT, sensitivity was similar to, while specificity was not as high as previously reported during a more stable context. Further studies are needed to evaluate its field performance, especially for different populations and other serogroups.Angela M C RoseSibylle GerstlAli E-H MahamaneFati SidikouSaacou DjiboLaurence BonteDominique A CaugantPhilippe J GuerinSuzanne ChanteauPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 10, p e7326 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Angela M C Rose Sibylle Gerstl Ali E-H Mahamane Fati Sidikou Saacou Djibo Laurence Bonte Dominique A Caugant Philippe J Guerin Suzanne Chanteau Field evaluation of two rapid diagnostic tests for Neisseria meningitidis serogroup A during the 2006 outbreak in Niger.
description	The Pastorex((R)) (BioRad) rapid agglutination test is one of the main rapid diagnostic tests (RDTs) for meningococcal disease currently in use in the "meningitis belt". Earlier evaluations, performed after heating and centrifugation of cerebrospinal fluid (CSF) samples, under good laboratory conditions, showed high sensitivity and specificity. However, during an epidemic, the test may be used without prior sample preparation. Recently a new, easy-to-use dipstick RDT for meningococcal disease detection on CSF was developed by the Centre de Recherche Médicale et Sanitaire in Niger and the Pasteur Institute in France. We estimate diagnostic accuracy in the field during the 2006 outbreak of Neisseria meningitidis serogroup A in Maradi, Niger, for the dipstick RDT and Pastorex((R)) on unprepared CSF, (a) by comparing each test's sensitivity and specificity with previously reported values; and (b) by comparing results for each test on paired samples, using McNemar's test. We also (c) estimate diagnostic accuracy of the dipstick RDT on diluted whole blood. We tested unprepared CSF and diluted whole blood from 126 patients with suspected meningococcal disease presenting at four health posts. (a) Pastorex((R)) sensitivity (69%; 95%CI 57-79) was significantly lower than found previously for prepared CSF samples [87% (81-91); or 88% (85-91)], as was specificity [81% (95%CI 68-91) vs 93% (90-95); or 93% (87-96)]. Sensitivity of the dipstick RDT [89% (95%CI 80-95)] was similar to previously reported values for ideal laboratory conditions [89% (84-93) and 94% (90-96)]. Specificity, at 62% (95%CI 48-75), was significantly lower than found previously [94% (92-96) and 97% (94-99)]. (b) McNemar's test for the dipstick RDT vs Pastorex((R)) was statistically significant (p<0.001). (c) The dipstick RDT did not perform satisfactorily on diluted whole blood (sensitivity 73%; specificity 57%).Sensitivity and specificity of Pastorex((R)) without prior CSF preparation were poorer than previously reported results from prepared samples; therefore we caution against using this test during an epidemic if sample preparation is not possible. For the dipstick RDT, sensitivity was similar to, while specificity was not as high as previously reported during a more stable context. Further studies are needed to evaluate its field performance, especially for different populations and other serogroups.
format	article
author	Angela M C Rose Sibylle Gerstl Ali E-H Mahamane Fati Sidikou Saacou Djibo Laurence Bonte Dominique A Caugant Philippe J Guerin Suzanne Chanteau
author_facet	Angela M C Rose Sibylle Gerstl Ali E-H Mahamane Fati Sidikou Saacou Djibo Laurence Bonte Dominique A Caugant Philippe J Guerin Suzanne Chanteau
author_sort	Angela M C Rose
title	Field evaluation of two rapid diagnostic tests for Neisseria meningitidis serogroup A during the 2006 outbreak in Niger.
title_short	Field evaluation of two rapid diagnostic tests for Neisseria meningitidis serogroup A during the 2006 outbreak in Niger.
title_full	Field evaluation of two rapid diagnostic tests for Neisseria meningitidis serogroup A during the 2006 outbreak in Niger.
title_fullStr	Field evaluation of two rapid diagnostic tests for Neisseria meningitidis serogroup A during the 2006 outbreak in Niger.
title_full_unstemmed	Field evaluation of two rapid diagnostic tests for Neisseria meningitidis serogroup A during the 2006 outbreak in Niger.
title_sort	field evaluation of two rapid diagnostic tests for neisseria meningitidis serogroup a during the 2006 outbreak in niger.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/9b3d839bac224b5fa097b3a230a5b23f
work_keys_str_mv	AT angelamcrose fieldevaluationoftworapiddiagnostictestsforneisseriameningitidisserogroupaduringthe2006outbreakinniger AT sibyllegerstl fieldevaluationoftworapiddiagnostictestsforneisseriameningitidisserogroupaduringthe2006outbreakinniger AT aliehmahamane fieldevaluationoftworapiddiagnostictestsforneisseriameningitidisserogroupaduringthe2006outbreakinniger AT fatisidikou fieldevaluationoftworapiddiagnostictestsforneisseriameningitidisserogroupaduringthe2006outbreakinniger AT saacoudjibo fieldevaluationoftworapiddiagnostictestsforneisseriameningitidisserogroupaduringthe2006outbreakinniger AT laurencebonte fieldevaluationoftworapiddiagnostictestsforneisseriameningitidisserogroupaduringthe2006outbreakinniger AT dominiqueacaugant fieldevaluationoftworapiddiagnostictestsforneisseriameningitidisserogroupaduringthe2006outbreakinniger AT philippejguerin fieldevaluationoftworapiddiagnostictestsforneisseriameningitidisserogroupaduringthe2006outbreakinniger AT suzannechanteau fieldevaluationoftworapiddiagnostictestsforneisseriameningitidisserogroupaduringthe2006outbreakinniger
_version_	1718413816078794752

Field evaluation of two rapid diagnostic tests for Neisseria meningitidis serogroup A during the 2006 outbreak in Niger.

Ejemplares similares