A class I odorant receptor enhancer shares a functional motif with class II enhancers

Abstract In the mouse, 129 functional class I odorant receptor (OR) genes reside in a ~ 3 megabase huge gene cluster on chromosome 7. The J element, a long-range cis-regulatory element governs the singular expression of class I OR genes by exerting its effect over the whole cluster. To elucidate the...

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Autores principales:	Tetsuo Iwata, Satoshi Tomeoka, Junji Hirota
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b3dd9640ae447dd9ebacee6f028c225
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spelling	oai:doaj.org-article:9b3dd9640ae447dd9ebacee6f028c2252021-12-02T14:12:08ZA class I odorant receptor enhancer shares a functional motif with class II enhancers10.1038/s41598-020-79980-x2045-2322https://doaj.org/article/9b3dd9640ae447dd9ebacee6f028c2252021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79980-xhttps://doaj.org/toc/2045-2322Abstract In the mouse, 129 functional class I odorant receptor (OR) genes reside in a ~ 3 megabase huge gene cluster on chromosome 7. The J element, a long-range cis-regulatory element governs the singular expression of class I OR genes by exerting its effect over the whole cluster. To elucidate the molecular mechanisms underlying class I-specific enhancer activity of the J element, we analyzed the J element sequence to determine the functional region and essential motif. The 430-bp core J element, that is highly conserved in mammalian species from the platypus to humans, contains a class I-specific conserved motif of AAACTTTTC, multiple homeodomain sites, and a neighboring O/E-like site, as in class II OR-enhancers. A series of transgenic reporter assays demonstrated that the class I-specific motif is not essential, but the 330-bp core J-H/O containing the homeodomain and O/E-like sites is necessary and sufficient for class I-specific enhancer activity. Further motif analysis revealed that one of homeodomain sequence is the Greek Islands composite motif of the adjacent homeodomain and O/E-like sequences, and mutations in the composite motif abolished or severely reduced class I-enhancer activity. Our results demonstrate that class I and class II enhancers share a functional motif for their enhancer activity.Tetsuo IwataSatoshi TomeokaJunji HirotaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tetsuo Iwata Satoshi Tomeoka Junji Hirota A class I odorant receptor enhancer shares a functional motif with class II enhancers
description	Abstract In the mouse, 129 functional class I odorant receptor (OR) genes reside in a ~ 3 megabase huge gene cluster on chromosome 7. The J element, a long-range cis-regulatory element governs the singular expression of class I OR genes by exerting its effect over the whole cluster. To elucidate the molecular mechanisms underlying class I-specific enhancer activity of the J element, we analyzed the J element sequence to determine the functional region and essential motif. The 430-bp core J element, that is highly conserved in mammalian species from the platypus to humans, contains a class I-specific conserved motif of AAACTTTTC, multiple homeodomain sites, and a neighboring O/E-like site, as in class II OR-enhancers. A series of transgenic reporter assays demonstrated that the class I-specific motif is not essential, but the 330-bp core J-H/O containing the homeodomain and O/E-like sites is necessary and sufficient for class I-specific enhancer activity. Further motif analysis revealed that one of homeodomain sequence is the Greek Islands composite motif of the adjacent homeodomain and O/E-like sequences, and mutations in the composite motif abolished or severely reduced class I-enhancer activity. Our results demonstrate that class I and class II enhancers share a functional motif for their enhancer activity.
format	article
author	Tetsuo Iwata Satoshi Tomeoka Junji Hirota
author_facet	Tetsuo Iwata Satoshi Tomeoka Junji Hirota
author_sort	Tetsuo Iwata
title	A class I odorant receptor enhancer shares a functional motif with class II enhancers
title_short	A class I odorant receptor enhancer shares a functional motif with class II enhancers
title_full	A class I odorant receptor enhancer shares a functional motif with class II enhancers
title_fullStr	A class I odorant receptor enhancer shares a functional motif with class II enhancers
title_full_unstemmed	A class I odorant receptor enhancer shares a functional motif with class II enhancers
title_sort	class i odorant receptor enhancer shares a functional motif with class ii enhancers
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9b3dd9640ae447dd9ebacee6f028c225
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A class I odorant receptor enhancer shares a functional motif with class II enhancers

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