Porphyromonas gingivalis and Treponema denticola exhibit metabolic symbioses.

Porphyromonas gingivalis and Treponema denticola are strongly associated with chronic periodontitis. These bacteria have been co-localized in subgingival plaque and demonstrated to exhibit symbiosis in growth in vitro and synergistic virulence upon co-infection in animal models of disease. Here we s...

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Autores principales:	Kheng H Tan, Christine A Seers, Stuart G Dashper, Helen L Mitchell, James S Pyke, Vincent Meuric, Nada Slakeski, Steven M Cleal, Jenny L Chambers, Malcolm J McConville, Eric C Reynolds
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9b45a54bde574be896440d0d9b95ffc2
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spelling	oai:doaj.org-article:9b45a54bde574be896440d0d9b95ffc22021-11-18T06:06:52ZPorphyromonas gingivalis and Treponema denticola exhibit metabolic symbioses.1553-73661553-737410.1371/journal.ppat.1003955https://doaj.org/article/9b45a54bde574be896440d0d9b95ffc22014-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24603978/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Porphyromonas gingivalis and Treponema denticola are strongly associated with chronic periodontitis. These bacteria have been co-localized in subgingival plaque and demonstrated to exhibit symbiosis in growth in vitro and synergistic virulence upon co-infection in animal models of disease. Here we show that during continuous co-culture a P. gingivalis:T. denticola cell ratio of 6∶1 was maintained with a respective increase of 54% and 30% in cell numbers when compared with mono-culture. Co-culture caused significant changes in global gene expression in both species with altered expression of 184 T. denticola and 134 P. gingivalis genes. P. gingivalis genes encoding a predicted thiamine biosynthesis pathway were up-regulated whilst genes involved in fatty acid biosynthesis were down-regulated. T. denticola genes encoding virulence factors including dentilisin and glycine catabolic pathways were significantly up-regulated during co-culture. Metabolic labeling using 13C-glycine showed that T. denticola rapidly metabolized this amino acid resulting in the production of acetate and lactate. P. gingivalis may be an important source of free glycine for T. denticola as mono-cultures of P. gingivalis and T. denticola were found to produce and consume free glycine, respectively; free glycine production by P. gingivalis was stimulated by T. denticola conditioned medium and glycine supplementation of T. denticola medium increased final cell density 1.7-fold. Collectively these data show P. gingivalis and T. denticola respond metabolically to the presence of each other with T. denticola displaying responses that help explain enhanced virulence of co-infections.Kheng H TanChristine A SeersStuart G DashperHelen L MitchellJames S PykeVincent MeuricNada SlakeskiSteven M ClealJenny L ChambersMalcolm J McConvilleEric C ReynoldsPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 10, Iss 3, p e1003955 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Kheng H Tan Christine A Seers Stuart G Dashper Helen L Mitchell James S Pyke Vincent Meuric Nada Slakeski Steven M Cleal Jenny L Chambers Malcolm J McConville Eric C Reynolds Porphyromonas gingivalis and Treponema denticola exhibit metabolic symbioses.
description	Porphyromonas gingivalis and Treponema denticola are strongly associated with chronic periodontitis. These bacteria have been co-localized in subgingival plaque and demonstrated to exhibit symbiosis in growth in vitro and synergistic virulence upon co-infection in animal models of disease. Here we show that during continuous co-culture a P. gingivalis:T. denticola cell ratio of 6∶1 was maintained with a respective increase of 54% and 30% in cell numbers when compared with mono-culture. Co-culture caused significant changes in global gene expression in both species with altered expression of 184 T. denticola and 134 P. gingivalis genes. P. gingivalis genes encoding a predicted thiamine biosynthesis pathway were up-regulated whilst genes involved in fatty acid biosynthesis were down-regulated. T. denticola genes encoding virulence factors including dentilisin and glycine catabolic pathways were significantly up-regulated during co-culture. Metabolic labeling using 13C-glycine showed that T. denticola rapidly metabolized this amino acid resulting in the production of acetate and lactate. P. gingivalis may be an important source of free glycine for T. denticola as mono-cultures of P. gingivalis and T. denticola were found to produce and consume free glycine, respectively; free glycine production by P. gingivalis was stimulated by T. denticola conditioned medium and glycine supplementation of T. denticola medium increased final cell density 1.7-fold. Collectively these data show P. gingivalis and T. denticola respond metabolically to the presence of each other with T. denticola displaying responses that help explain enhanced virulence of co-infections.
format	article
author	Kheng H Tan Christine A Seers Stuart G Dashper Helen L Mitchell James S Pyke Vincent Meuric Nada Slakeski Steven M Cleal Jenny L Chambers Malcolm J McConville Eric C Reynolds
author_facet	Kheng H Tan Christine A Seers Stuart G Dashper Helen L Mitchell James S Pyke Vincent Meuric Nada Slakeski Steven M Cleal Jenny L Chambers Malcolm J McConville Eric C Reynolds
author_sort	Kheng H Tan
title	Porphyromonas gingivalis and Treponema denticola exhibit metabolic symbioses.
title_short	Porphyromonas gingivalis and Treponema denticola exhibit metabolic symbioses.
title_full	Porphyromonas gingivalis and Treponema denticola exhibit metabolic symbioses.
title_fullStr	Porphyromonas gingivalis and Treponema denticola exhibit metabolic symbioses.
title_full_unstemmed	Porphyromonas gingivalis and Treponema denticola exhibit metabolic symbioses.
title_sort	porphyromonas gingivalis and treponema denticola exhibit metabolic symbioses.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/9b45a54bde574be896440d0d9b95ffc2
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Porphyromonas gingivalis and Treponema denticola exhibit metabolic symbioses.

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