A synthetic urinary probe–coated nanoparticles sensitive to fibroblast activation protein α for solid tumor diagnosis
Xinwei Feng,1,* Qifan Wang,1,* Yuehua Liao,1 Xie Zhou,2 Yidan Wang,3 Wanli Liu,4 Ge Zhang1 1Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 2Department of Medical Chemistry, School of Pharmaceutical Sciences, Sun Yat-sen Univer...
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Dove Medical Press
2017
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oai:doaj.org-article:9b477e2a4791466e841be2aa4bdd5c5f2021-12-02T03:11:32ZA synthetic urinary probe–coated nanoparticles sensitive to fibroblast activation protein α for solid tumor diagnosis1178-2013https://doaj.org/article/9b477e2a4791466e841be2aa4bdd5c5f2017-07-01T00:00:00Zhttps://www.dovepress.com/a-synthetic-urinary-probe-coated-nanoparticles-sensitive-to-fibroblast-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xinwei Feng,1,* Qifan Wang,1,* Yuehua Liao,1 Xie Zhou,2 Yidan Wang,3 Wanli Liu,4 Ge Zhang1 1Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 2Department of Medical Chemistry, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 3Department of Biotechnology, School of Life Science, Sun Yat-sen University, Guangzhou, 4Department of Clinical Laboratory Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China *These authors contributed equally to this work Abstract: We developed fibroblast activation protein α (FAPα)-sensitive magnetic iron oxide nanoparticles (MNPs) by conjugating a substrate-reporter tandem peptide as a synthetic biomarker to the surface of MNPs (marker-MNPs). In vitro, the marker-MNPs showed stability when treated with serum or urine and exhibited high susceptibility and specificity for FAPα enzyme and 3T3/FAPα cell line. Furthermore, the marker-MNPs were administered to esophageal squamous cell carcinoma xenograft tumor mice; they reached the tumor tissues in the mice, where they were cleaved effectively by the local overexpressed FAPα to release the reporter peptide and filter it into the urine. The tumor targeting and biodistribution of marker-MNPs were verified by in vivo imaging. The cleaved reporter peptides in urine detected by enzyme-linked immunosorbent assay have high diagnostic accuracy for esophageal squamous cell carcinoma (area under the receiver-operating characteristic curve =1.0). Our study implies a promising strategy of utilizing the low-cost and noninvasive synthetic urinary probe–coated nanoparticles for the diagnosis of FAPα-positive solid tumors, except for in renal cancer. Keywords: synthetic urinary probe, magnetic iron oxide nanoparticles, fibroblast activation protein α, tumor diagnosisFeng XWWang QFLiao YHZhou XWang YDLiu WLZhang GDove Medical PressarticleSynthetic urinary biomarkerMagnetic iron oxide nanoparticlesFibroblast activation protein-αTumor diagnosis.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 5359-5372 (2017) |
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Synthetic urinary biomarker Magnetic iron oxide nanoparticles Fibroblast activation protein-α Tumor diagnosis. Medicine (General) R5-920 |
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Synthetic urinary biomarker Magnetic iron oxide nanoparticles Fibroblast activation protein-α Tumor diagnosis. Medicine (General) R5-920 Feng XW Wang QF Liao YH Zhou X Wang YD Liu WL Zhang G A synthetic urinary probe–coated nanoparticles sensitive to fibroblast activation protein α for solid tumor diagnosis |
| description |
Xinwei Feng,1,* Qifan Wang,1,* Yuehua Liao,1 Xie Zhou,2 Yidan Wang,3 Wanli Liu,4 Ge Zhang1 1Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 2Department of Medical Chemistry, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 3Department of Biotechnology, School of Life Science, Sun Yat-sen University, Guangzhou, 4Department of Clinical Laboratory Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China *These authors contributed equally to this work Abstract: We developed fibroblast activation protein α (FAPα)-sensitive magnetic iron oxide nanoparticles (MNPs) by conjugating a substrate-reporter tandem peptide as a synthetic biomarker to the surface of MNPs (marker-MNPs). In vitro, the marker-MNPs showed stability when treated with serum or urine and exhibited high susceptibility and specificity for FAPα enzyme and 3T3/FAPα cell line. Furthermore, the marker-MNPs were administered to esophageal squamous cell carcinoma xenograft tumor mice; they reached the tumor tissues in the mice, where they were cleaved effectively by the local overexpressed FAPα to release the reporter peptide and filter it into the urine. The tumor targeting and biodistribution of marker-MNPs were verified by in vivo imaging. The cleaved reporter peptides in urine detected by enzyme-linked immunosorbent assay have high diagnostic accuracy for esophageal squamous cell carcinoma (area under the receiver-operating characteristic curve =1.0). Our study implies a promising strategy of utilizing the low-cost and noninvasive synthetic urinary probe–coated nanoparticles for the diagnosis of FAPα-positive solid tumors, except for in renal cancer. Keywords: synthetic urinary probe, magnetic iron oxide nanoparticles, fibroblast activation protein α, tumor diagnosis |
| format |
article |
| author |
Feng XW Wang QF Liao YH Zhou X Wang YD Liu WL Zhang G |
| author_facet |
Feng XW Wang QF Liao YH Zhou X Wang YD Liu WL Zhang G |
| author_sort |
Feng XW |
| title |
A synthetic urinary probe–coated nanoparticles sensitive to fibroblast activation protein α for solid tumor diagnosis |
| title_short |
A synthetic urinary probe–coated nanoparticles sensitive to fibroblast activation protein α for solid tumor diagnosis |
| title_full |
A synthetic urinary probe–coated nanoparticles sensitive to fibroblast activation protein α for solid tumor diagnosis |
| title_fullStr |
A synthetic urinary probe–coated nanoparticles sensitive to fibroblast activation protein α for solid tumor diagnosis |
| title_full_unstemmed |
A synthetic urinary probe–coated nanoparticles sensitive to fibroblast activation protein α for solid tumor diagnosis |
| title_sort |
synthetic urinary probe–coated nanoparticles sensitive to fibroblast activation protein α for solid tumor diagnosis |
| publisher |
Dove Medical Press |
| publishDate |
2017 |
| url |
https://doaj.org/article/9b477e2a4791466e841be2aa4bdd5c5f |
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