Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays
Objective. To estimate performance characteristics and diagnostic value of immunofluorescent islet cell antibody (ICA) assay, immunoradiometric glutamic acid decarboxylase antibody (GADA) assay, and ELISA tyrosine phosphatase IA-2 antibody (IA-2A) and insulin antibody (IA) assays.Research Design and...
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Endocrinology Research Centre
2016
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oai:doaj.org-article:9b477f127dc84b3cbb0b91b1699fd6582021-11-14T09:00:20ZDiagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays2072-03512072-037810.14341/DM8032https://doaj.org/article/9b477f127dc84b3cbb0b91b1699fd6582016-11-01T00:00:00Zhttps://www.dia-endojournals.ru/jour/article/view/8032https://doaj.org/toc/2072-0351https://doaj.org/toc/2072-0378Objective. To estimate performance characteristics and diagnostic value of immunofluorescent islet cell antibody (ICA) assay, immunoradiometric glutamic acid decarboxylase antibody (GADA) assay, and ELISA tyrosine phosphatase IA-2 antibody (IA-2A) and insulin antibody (IA) assays.Research Design and Methods. Antibodies were tested in 438 children and adolescents with newly diagnosed diabetes mellitus (DM) type 1, and in 891 subjects without DM type 1. ICA were determined by the classic indirect immunofluorescent method recommended by the Juvenile Diabetes Foundation International, GADA were determined with the Immunotech IRMA Anti-GAD kit, and IA-2A and IA were determined with Medizym Anti-IA2 and Orgentec Anti-Insulin ELISA kits, respectively. Sensitivity (Se), specificity (Sp), positive predictive value (PPV) and negative predictive value (NPV) of the tests were estimated with contingency tables. Diagnostic accuracy was estimated from areas under receiver operating curves (AUC).Results. ICA test was of the greatest diagnostic value (Se=88%, Sp=96%, PPV=96%, NPV=94%, AUC=0,94), followed by IA-2A (Se=66%, Sp=98%, PPV=98%, NPV=59%, AUC=0,82) and GADA (Se=73%, Sp=84%, PPV=75%, NPV=83%, AUC=0,79). IA test exhibited a very low Se (4,3%) and lacked diagnostic accuracy (AUC=0,5).Conclusions. We recommend to use ICA, IA-2A and GADA tests surveyed in our study for diagnosis of DM type 1 and differential diagnosis of DM. We don’t recommend IA testing with an Orgentec Anti-Insulin ELISA kit for usage in clinical practice.Alexei V. TimofeevKsenia A. GorstValentin Y. UvarovEkaterina A. ProninaAlisa V. VitebskayaAnastasiya V. PopovichAnatoliy N. TiulpakovNatalia A. ZubkovaOlesya A. GioevaYulia V. TikhonovichAlexander V. IlinOleg Y. LatyshevGoar F. OkminjanKsenia L. KabolovaJulianna A. KanokovaLeokadia Y. ZhulevaIgor E. KoltunovElena E. PetriaikinaIrina G. RybkinaIrina V. GaryaevaSalome L. GuguchiaAngelina B. ShimarovaArtiom V. BullikhIrina G. KolominaEvgenia A. EvsyukovaSergei S. BukinNatalia N. EgarminaNatalia Y. ArbatskayaElina Y. YanovskayaVladimir I. PopenkoAlexander A. ArkhipkinEndocrinology Research Centrearticlediabetes mellitusdiabetes mellitus type 1differential diagnosisautoantibodiesislet cell antibodyglutamic acid decarboxylase antibodytyrosine phosphatase antibodyinsulin antibodyimmunofluorescence assayradioimmune assayimmunoenzyme assaNutritional diseases. Deficiency diseasesRC620-627ENRUСахарный диабет, Vol 19, Iss 4, Pp 331-340 (2016) |
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diabetes mellitus diabetes mellitus type 1 differential diagnosis autoantibodies islet cell antibody glutamic acid decarboxylase antibody tyrosine phosphatase antibody insulin antibody immunofluorescence assay radioimmune assay immunoenzyme assa Nutritional diseases. Deficiency diseases RC620-627 |
| spellingShingle |
diabetes mellitus diabetes mellitus type 1 differential diagnosis autoantibodies islet cell antibody glutamic acid decarboxylase antibody tyrosine phosphatase antibody insulin antibody immunofluorescence assay radioimmune assay immunoenzyme assa Nutritional diseases. Deficiency diseases RC620-627 Alexei V. Timofeev Ksenia A. Gorst Valentin Y. Uvarov Ekaterina A. Pronina Alisa V. Vitebskaya Anastasiya V. Popovich Anatoliy N. Tiulpakov Natalia A. Zubkova Olesya A. Gioeva Yulia V. Tikhonovich Alexander V. Ilin Oleg Y. Latyshev Goar F. Okminjan Ksenia L. Kabolova Julianna A. Kanokova Leokadia Y. Zhuleva Igor E. Koltunov Elena E. Petriaikina Irina G. Rybkina Irina V. Garyaeva Salome L. Guguchia Angelina B. Shimarova Artiom V. Bullikh Irina G. Kolomina Evgenia A. Evsyukova Sergei S. Bukin Natalia N. Egarmina Natalia Y. Arbatskaya Elina Y. Yanovskaya Vladimir I. Popenko Alexander A. Arkhipkin Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays |
| description |
Objective. To estimate performance characteristics and diagnostic value of immunofluorescent islet cell antibody (ICA) assay, immunoradiometric glutamic acid decarboxylase antibody (GADA) assay, and ELISA tyrosine phosphatase IA-2 antibody (IA-2A) and insulin antibody (IA) assays.Research Design and Methods. Antibodies were tested in 438 children and adolescents with newly diagnosed diabetes mellitus (DM) type 1, and in 891 subjects without DM type 1. ICA were determined by the classic indirect immunofluorescent method recommended by the Juvenile Diabetes Foundation International, GADA were determined with the Immunotech IRMA Anti-GAD kit, and IA-2A and IA were determined with Medizym Anti-IA2 and Orgentec Anti-Insulin ELISA kits, respectively. Sensitivity (Se), specificity (Sp), positive predictive value (PPV) and negative predictive value (NPV) of the tests were estimated with contingency tables. Diagnostic accuracy was estimated from areas under receiver operating curves (AUC).Results. ICA test was of the greatest diagnostic value (Se=88%, Sp=96%, PPV=96%, NPV=94%, AUC=0,94), followed by IA-2A (Se=66%, Sp=98%, PPV=98%, NPV=59%, AUC=0,82) and GADA (Se=73%, Sp=84%, PPV=75%, NPV=83%, AUC=0,79). IA test exhibited a very low Se (4,3%) and lacked diagnostic accuracy (AUC=0,5).Conclusions. We recommend to use ICA, IA-2A and GADA tests surveyed in our study for diagnosis of DM type 1 and differential diagnosis of DM. We don’t recommend IA testing with an Orgentec Anti-Insulin ELISA kit for usage in clinical practice. |
| format |
article |
| author |
Alexei V. Timofeev Ksenia A. Gorst Valentin Y. Uvarov Ekaterina A. Pronina Alisa V. Vitebskaya Anastasiya V. Popovich Anatoliy N. Tiulpakov Natalia A. Zubkova Olesya A. Gioeva Yulia V. Tikhonovich Alexander V. Ilin Oleg Y. Latyshev Goar F. Okminjan Ksenia L. Kabolova Julianna A. Kanokova Leokadia Y. Zhuleva Igor E. Koltunov Elena E. Petriaikina Irina G. Rybkina Irina V. Garyaeva Salome L. Guguchia Angelina B. Shimarova Artiom V. Bullikh Irina G. Kolomina Evgenia A. Evsyukova Sergei S. Bukin Natalia N. Egarmina Natalia Y. Arbatskaya Elina Y. Yanovskaya Vladimir I. Popenko Alexander A. Arkhipkin |
| author_facet |
Alexei V. Timofeev Ksenia A. Gorst Valentin Y. Uvarov Ekaterina A. Pronina Alisa V. Vitebskaya Anastasiya V. Popovich Anatoliy N. Tiulpakov Natalia A. Zubkova Olesya A. Gioeva Yulia V. Tikhonovich Alexander V. Ilin Oleg Y. Latyshev Goar F. Okminjan Ksenia L. Kabolova Julianna A. Kanokova Leokadia Y. Zhuleva Igor E. Koltunov Elena E. Petriaikina Irina G. Rybkina Irina V. Garyaeva Salome L. Guguchia Angelina B. Shimarova Artiom V. Bullikh Irina G. Kolomina Evgenia A. Evsyukova Sergei S. Bukin Natalia N. Egarmina Natalia Y. Arbatskaya Elina Y. Yanovskaya Vladimir I. Popenko Alexander A. Arkhipkin |
| author_sort |
Alexei V. Timofeev |
| title |
Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays |
| title_short |
Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays |
| title_full |
Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays |
| title_fullStr |
Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays |
| title_full_unstemmed |
Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays |
| title_sort |
diagnostic value of islet autoantibody assays practised in russia. 1. classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and elisa tyrosine phosphatase antibody and insulin antibody assays |
| publisher |
Endocrinology Research Centre |
| publishDate |
2016 |
| url |
https://doaj.org/article/9b477f127dc84b3cbb0b91b1699fd658 |
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