Antibacterial activity testing methods for hydrophobic patterned surfaces

Abstract One strategy to decrease the incidence of hospital-acquired infections is to avoid the survival of pathogens in the environment by the development of surfaces with antimicrobial activity. To study the antibacterial behaviour of active surfaces, different approaches have been developed of wh...

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Autores principales:	Ana Perez-Gavilan, Joana Vieira de Castro, Ainara Arana, Santos Merino, Aritz Retolaza, Sofia A. Alves, Achille Francone, Nikolaos Kehagias, Clivia M. Sotomayor-Torres, Donato Cocina, Renato Mortera, Salvatore Crapanzano, Carlos Javier Pelegrín, María Carmen Garrigos, Alfonso Jiménez, Begoña Galindo, Mari Carmen Araque, Donna Dykeman, Nuno M. Neves, Jose Maria Marimón
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b49e2ef7e5f4f3898b48d782921b5b2
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spelling	oai:doaj.org-article:9b49e2ef7e5f4f3898b48d782921b5b22021-12-02T13:24:25ZAntibacterial activity testing methods for hydrophobic patterned surfaces10.1038/s41598-021-85995-92045-2322https://doaj.org/article/9b49e2ef7e5f4f3898b48d782921b5b22021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85995-9https://doaj.org/toc/2045-2322Abstract One strategy to decrease the incidence of hospital-acquired infections is to avoid the survival of pathogens in the environment by the development of surfaces with antimicrobial activity. To study the antibacterial behaviour of active surfaces, different approaches have been developed of which ISO 22916 is the standard. To assess the performance of different testing methodologies to analyse the antibacterial activity of hydrophobic surface patterned plastics as part of a Horizon 2020 European research project. Four different testing methods were used to study the antibacterial activity of a patterned film, including the ISO 22916 standard, the immersion method, the touch-transfer inoculation method, and the swab inoculation method, this latter developed specifically for this project. The non-realistic test conditions of the ISO 22916 standard showed this method to be non-appropriate in the study of hydrophobic patterned surfaces. The immersion method also showed no differences between patterned films and smooth controls due to the lack of attachment of testing bacteria on both surfaces. The antibacterial activity of films could be demonstrated by the touch-transfer and the swab inoculation methods, that more precisely mimicked the way of high-touch surfaces contamination, and showed to be the best methodologies to test the antibacterial activity of patterned hydrophobic surfaces. A new ISO standard would be desirable as the reference method to study the antibacterial behaviour of patterned surfaces.Ana Perez-GavilanJoana Vieira de CastroAinara AranaSantos MerinoAritz RetolazaSofia A. AlvesAchille FranconeNikolaos KehagiasClivia M. Sotomayor-TorresDonato CocinaRenato MorteraSalvatore CrapanzanoCarlos Javier PelegrínMaría Carmen GarrigosAlfonso JiménezBegoña GalindoMari Carmen AraqueDonna DykemanNuno M. NevesJose Maria MarimónNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Perez-Gavilan Joana Vieira de Castro Ainara Arana Santos Merino Aritz Retolaza Sofia A. Alves Achille Francone Nikolaos Kehagias Clivia M. Sotomayor-Torres Donato Cocina Renato Mortera Salvatore Crapanzano Carlos Javier Pelegrín María Carmen Garrigos Alfonso Jiménez Begoña Galindo Mari Carmen Araque Donna Dykeman Nuno M. Neves Jose Maria Marimón Antibacterial activity testing methods for hydrophobic patterned surfaces
description	Abstract One strategy to decrease the incidence of hospital-acquired infections is to avoid the survival of pathogens in the environment by the development of surfaces with antimicrobial activity. To study the antibacterial behaviour of active surfaces, different approaches have been developed of which ISO 22916 is the standard. To assess the performance of different testing methodologies to analyse the antibacterial activity of hydrophobic surface patterned plastics as part of a Horizon 2020 European research project. Four different testing methods were used to study the antibacterial activity of a patterned film, including the ISO 22916 standard, the immersion method, the touch-transfer inoculation method, and the swab inoculation method, this latter developed specifically for this project. The non-realistic test conditions of the ISO 22916 standard showed this method to be non-appropriate in the study of hydrophobic patterned surfaces. The immersion method also showed no differences between patterned films and smooth controls due to the lack of attachment of testing bacteria on both surfaces. The antibacterial activity of films could be demonstrated by the touch-transfer and the swab inoculation methods, that more precisely mimicked the way of high-touch surfaces contamination, and showed to be the best methodologies to test the antibacterial activity of patterned hydrophobic surfaces. A new ISO standard would be desirable as the reference method to study the antibacterial behaviour of patterned surfaces.
format	article
author	Ana Perez-Gavilan Joana Vieira de Castro Ainara Arana Santos Merino Aritz Retolaza Sofia A. Alves Achille Francone Nikolaos Kehagias Clivia M. Sotomayor-Torres Donato Cocina Renato Mortera Salvatore Crapanzano Carlos Javier Pelegrín María Carmen Garrigos Alfonso Jiménez Begoña Galindo Mari Carmen Araque Donna Dykeman Nuno M. Neves Jose Maria Marimón
author_facet	Ana Perez-Gavilan Joana Vieira de Castro Ainara Arana Santos Merino Aritz Retolaza Sofia A. Alves Achille Francone Nikolaos Kehagias Clivia M. Sotomayor-Torres Donato Cocina Renato Mortera Salvatore Crapanzano Carlos Javier Pelegrín María Carmen Garrigos Alfonso Jiménez Begoña Galindo Mari Carmen Araque Donna Dykeman Nuno M. Neves Jose Maria Marimón
author_sort	Ana Perez-Gavilan
title	Antibacterial activity testing methods for hydrophobic patterned surfaces
title_short	Antibacterial activity testing methods for hydrophobic patterned surfaces
title_full	Antibacterial activity testing methods for hydrophobic patterned surfaces
title_fullStr	Antibacterial activity testing methods for hydrophobic patterned surfaces
title_full_unstemmed	Antibacterial activity testing methods for hydrophobic patterned surfaces
title_sort	antibacterial activity testing methods for hydrophobic patterned surfaces
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9b49e2ef7e5f4f3898b48d782921b5b2
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Antibacterial activity testing methods for hydrophobic patterned surfaces

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