A Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR ortholog is not crucial for Brucella abortus virulence.

Brucella is a facultative extracellular-intracellular pathogen that belongs to the Alphaproteobacteria class. Precise sensing of environmental changes and a proper response mediated by a gene expression regulatory network are essential for this pathogen to survive. The plant-related Alphaproteobacte...

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Autores principales:	Amanda Castillo-Zeledón, Nazareth Ruiz-Villalobos, Pamela Altamirano-Silva, Carlos Chacón-Díaz, Elías Barquero-Calvo, Esteban Chaves-Olarte, Caterina Guzmán-Verri
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b596ed25bcd4900a45d9cc4dae2ff33
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spelling	oai:doaj.org-article:9b596ed25bcd4900a45d9cc4dae2ff332021-12-02T20:18:11ZA Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR ortholog is not crucial for Brucella abortus virulence.1932-620310.1371/journal.pone.0254568https://doaj.org/article/9b596ed25bcd4900a45d9cc4dae2ff332021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254568https://doaj.org/toc/1932-6203Brucella is a facultative extracellular-intracellular pathogen that belongs to the Alphaproteobacteria class. Precise sensing of environmental changes and a proper response mediated by a gene expression regulatory network are essential for this pathogen to survive. The plant-related Alphaproteobacteria Sinorhizobium meliloti and Agrobacterium tumefaciens also alternate from a free to a host-associated life, where a regulatory invasion switch is needed for this transition. This switch is composed of a two-component regulatory system (TCS) and a global inhibitor, ExoR. In B. abortus, the BvrR/BvrS TCS is essential for intracellular survival. However, the presence of a TCS inhibitor, such as ExoR, in Brucella is still unknown. In this work, we identified a genomic sequence similar to S. meliloti exoR in the B. abortus 2308W genome, constructed an exoR mutant strain, and performed its characterization through ex vivo and in vivo assays. Our findings indicate that ExoR is related to the BvrR phosphorylation state, and is related to the expression of known BvrR/BrvS gene targets, such as virB8, vjbR, and omp25 when grown in rich medium or starving conditions. Despite this, the exoR mutant strain showed no significant differences as compared to the wild-type strain, related to resistance to polymyxin B or human non-immune serum, intracellular replication, or infectivity in a mice model. ExoR in B. abortus is related to BvrR/BvrS as observed in other Rhizobiales; however, its function seems different from that observed for its orthologs described in A. tumefaciens and S. meliloti.Amanda Castillo-ZeledónNazareth Ruiz-VillalobosPamela Altamirano-SilvaCarlos Chacón-DíazElías Barquero-CalvoEsteban Chaves-OlarteCaterina Guzmán-VerriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0254568 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amanda Castillo-Zeledón Nazareth Ruiz-Villalobos Pamela Altamirano-Silva Carlos Chacón-Díaz Elías Barquero-Calvo Esteban Chaves-Olarte Caterina Guzmán-Verri A Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR ortholog is not crucial for Brucella abortus virulence.
description	Brucella is a facultative extracellular-intracellular pathogen that belongs to the Alphaproteobacteria class. Precise sensing of environmental changes and a proper response mediated by a gene expression regulatory network are essential for this pathogen to survive. The plant-related Alphaproteobacteria Sinorhizobium meliloti and Agrobacterium tumefaciens also alternate from a free to a host-associated life, where a regulatory invasion switch is needed for this transition. This switch is composed of a two-component regulatory system (TCS) and a global inhibitor, ExoR. In B. abortus, the BvrR/BvrS TCS is essential for intracellular survival. However, the presence of a TCS inhibitor, such as ExoR, in Brucella is still unknown. In this work, we identified a genomic sequence similar to S. meliloti exoR in the B. abortus 2308W genome, constructed an exoR mutant strain, and performed its characterization through ex vivo and in vivo assays. Our findings indicate that ExoR is related to the BvrR phosphorylation state, and is related to the expression of known BvrR/BrvS gene targets, such as virB8, vjbR, and omp25 when grown in rich medium or starving conditions. Despite this, the exoR mutant strain showed no significant differences as compared to the wild-type strain, related to resistance to polymyxin B or human non-immune serum, intracellular replication, or infectivity in a mice model. ExoR in B. abortus is related to BvrR/BvrS as observed in other Rhizobiales; however, its function seems different from that observed for its orthologs described in A. tumefaciens and S. meliloti.
format	article
author	Amanda Castillo-Zeledón Nazareth Ruiz-Villalobos Pamela Altamirano-Silva Carlos Chacón-Díaz Elías Barquero-Calvo Esteban Chaves-Olarte Caterina Guzmán-Verri
author_facet	Amanda Castillo-Zeledón Nazareth Ruiz-Villalobos Pamela Altamirano-Silva Carlos Chacón-Díaz Elías Barquero-Calvo Esteban Chaves-Olarte Caterina Guzmán-Verri
author_sort	Amanda Castillo-Zeledón
title	A Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR ortholog is not crucial for Brucella abortus virulence.
title_short	A Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR ortholog is not crucial for Brucella abortus virulence.
title_full	A Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR ortholog is not crucial for Brucella abortus virulence.
title_fullStr	A Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR ortholog is not crucial for Brucella abortus virulence.
title_full_unstemmed	A Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR ortholog is not crucial for Brucella abortus virulence.
title_sort	sinorhizobium meliloti and agrobacterium tumefaciens exor ortholog is not crucial for brucella abortus virulence.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/9b596ed25bcd4900a45d9cc4dae2ff33
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A Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR ortholog is not crucial for Brucella abortus virulence.

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