Local immunization impacts the response of dairy cows to Escherichia coli mastitis

Abstract Current vaccines to Escherichia coli mastitis have shown some albeit limited efficacy. Their mode of action has not been documented, and immune responses protecting the mammary gland against E. coli are not completely understood. To improve our knowledge of mammary gland immune protection,...

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Autores principales:	Vincent Herry, Christophe Gitton, Guillaume Tabouret, Maryline Répérant, Laurine Forge, Christian Tasca, Florence B. Gilbert, Edouard Guitton, Céline Barc, Christophe Staub, David G. E. Smith, Pierre Germon, Gilles Foucras, Pascal Rainard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b5bce202dec4b3c8277e2409e888ca7
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spelling	oai:doaj.org-article:9b5bce202dec4b3c8277e2409e888ca72021-12-02T12:32:43ZLocal immunization impacts the response of dairy cows to Escherichia coli mastitis10.1038/s41598-017-03724-72045-2322https://doaj.org/article/9b5bce202dec4b3c8277e2409e888ca72017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03724-7https://doaj.org/toc/2045-2322Abstract Current vaccines to Escherichia coli mastitis have shown some albeit limited efficacy. Their mode of action has not been documented, and immune responses protecting the mammary gland against E. coli are not completely understood. To improve our knowledge of mammary gland immune protection, cows immunized either intramuscularly or intramammarily with the E. coli P4 were submitted to a homologous mastitis challenge. A third group of mock-immunized cows serve as challenge controls. Local immunization modified favorably the course of infection, by improving bacterial clearance while limiting inflammation. Systemic clinical signs and reduction in milk secretion were also contained. This occurred with a modification of the cytokine profile, such as an increase in IFN-γ and a reduction in TNF-α concentrations in milk. Concentrations of IL-17A and IL-22 increased in milk at the onset of the inflammatory response and remained high up to the elimination of bacteria, but concentrations did not differ between groups. Accelerated bacteriological cure was not linked to an increase in the initial efficiency of phagocytosis in milk. Results support the idea that antibodies did not play a major role in the improvement, and that cell-mediated immunity is the key to understanding E. coli vaccine-induced protection of the mammary gland.Vincent HerryChristophe GittonGuillaume TabouretMaryline RépérantLaurine ForgeChristian TascaFlorence B. GilbertEdouard GuittonCéline BarcChristophe StaubDavid G. E. SmithPierre GermonGilles FoucrasPascal RainardNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-18 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vincent Herry Christophe Gitton Guillaume Tabouret Maryline Répérant Laurine Forge Christian Tasca Florence B. Gilbert Edouard Guitton Céline Barc Christophe Staub David G. E. Smith Pierre Germon Gilles Foucras Pascal Rainard Local immunization impacts the response of dairy cows to Escherichia coli mastitis
description	Abstract Current vaccines to Escherichia coli mastitis have shown some albeit limited efficacy. Their mode of action has not been documented, and immune responses protecting the mammary gland against E. coli are not completely understood. To improve our knowledge of mammary gland immune protection, cows immunized either intramuscularly or intramammarily with the E. coli P4 were submitted to a homologous mastitis challenge. A third group of mock-immunized cows serve as challenge controls. Local immunization modified favorably the course of infection, by improving bacterial clearance while limiting inflammation. Systemic clinical signs and reduction in milk secretion were also contained. This occurred with a modification of the cytokine profile, such as an increase in IFN-γ and a reduction in TNF-α concentrations in milk. Concentrations of IL-17A and IL-22 increased in milk at the onset of the inflammatory response and remained high up to the elimination of bacteria, but concentrations did not differ between groups. Accelerated bacteriological cure was not linked to an increase in the initial efficiency of phagocytosis in milk. Results support the idea that antibodies did not play a major role in the improvement, and that cell-mediated immunity is the key to understanding E. coli vaccine-induced protection of the mammary gland.
format	article
author	Vincent Herry Christophe Gitton Guillaume Tabouret Maryline Répérant Laurine Forge Christian Tasca Florence B. Gilbert Edouard Guitton Céline Barc Christophe Staub David G. E. Smith Pierre Germon Gilles Foucras Pascal Rainard
author_facet	Vincent Herry Christophe Gitton Guillaume Tabouret Maryline Répérant Laurine Forge Christian Tasca Florence B. Gilbert Edouard Guitton Céline Barc Christophe Staub David G. E. Smith Pierre Germon Gilles Foucras Pascal Rainard
author_sort	Vincent Herry
title	Local immunization impacts the response of dairy cows to Escherichia coli mastitis
title_short	Local immunization impacts the response of dairy cows to Escherichia coli mastitis
title_full	Local immunization impacts the response of dairy cows to Escherichia coli mastitis
title_fullStr	Local immunization impacts the response of dairy cows to Escherichia coli mastitis
title_full_unstemmed	Local immunization impacts the response of dairy cows to Escherichia coli mastitis
title_sort	local immunization impacts the response of dairy cows to escherichia coli mastitis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9b5bce202dec4b3c8277e2409e888ca7
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Local immunization impacts the response of dairy cows to Escherichia coli mastitis

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