Antibody cross-reactivity accounts for widespread appearance of m1A in 5’UTRs

N 1-methyladenosine (m1A) was recently reported as a new mRNA modification but its prevalence has been controversial. Here the authors showed that m1A, if present in mRNA, is at very low stoichiometry, with the notable exception of MT-ND5. Further, they show that the previously reported enrichment o...

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Autores principales:	Anya V. Grozhik, Anthony O. Olarerin-George, Miriam Sindelar, Xing Li, Steven S. Gross, Samie R. Jaffrey
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9b5c924dc48f490aafd659539f03fc90
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spelling	oai:doaj.org-article:9b5c924dc48f490aafd659539f03fc902021-12-02T17:32:54ZAntibody cross-reactivity accounts for widespread appearance of m1A in 5’UTRs10.1038/s41467-019-13146-w2041-1723https://doaj.org/article/9b5c924dc48f490aafd659539f03fc902019-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13146-whttps://doaj.org/toc/2041-1723N 1-methyladenosine (m1A) was recently reported as a new mRNA modification but its prevalence has been controversial. Here the authors showed that m1A, if present in mRNA, is at very low stoichiometry, with the notable exception of MT-ND5. Further, they show that the previously reported enrichment of m1A near the start of transcripts are false-positive identifications due to cross-reactivity of the commonly used m1A antibody with mRNA caps.Anya V. GrozhikAnthony O. Olarerin-GeorgeMiriam SindelarXing LiSteven S. GrossSamie R. JaffreyNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Anya V. Grozhik Anthony O. Olarerin-George Miriam Sindelar Xing Li Steven S. Gross Samie R. Jaffrey Antibody cross-reactivity accounts for widespread appearance of m1A in 5’UTRs
description	N 1-methyladenosine (m1A) was recently reported as a new mRNA modification but its prevalence has been controversial. Here the authors showed that m1A, if present in mRNA, is at very low stoichiometry, with the notable exception of MT-ND5. Further, they show that the previously reported enrichment of m1A near the start of transcripts are false-positive identifications due to cross-reactivity of the commonly used m1A antibody with mRNA caps.
format	article
author	Anya V. Grozhik Anthony O. Olarerin-George Miriam Sindelar Xing Li Steven S. Gross Samie R. Jaffrey
author_facet	Anya V. Grozhik Anthony O. Olarerin-George Miriam Sindelar Xing Li Steven S. Gross Samie R. Jaffrey
author_sort	Anya V. Grozhik
title	Antibody cross-reactivity accounts for widespread appearance of m1A in 5’UTRs
title_short	Antibody cross-reactivity accounts for widespread appearance of m1A in 5’UTRs
title_full	Antibody cross-reactivity accounts for widespread appearance of m1A in 5’UTRs
title_fullStr	Antibody cross-reactivity accounts for widespread appearance of m1A in 5’UTRs
title_full_unstemmed	Antibody cross-reactivity accounts for widespread appearance of m1A in 5’UTRs
title_sort	antibody cross-reactivity accounts for widespread appearance of m1a in 5’utrs
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/9b5c924dc48f490aafd659539f03fc90
work_keys_str_mv	AT anyavgrozhik antibodycrossreactivityaccountsforwidespreadappearanceofm1ain5utrs AT anthonyoolareringeorge antibodycrossreactivityaccountsforwidespreadappearanceofm1ain5utrs AT miriamsindelar antibodycrossreactivityaccountsforwidespreadappearanceofm1ain5utrs AT xingli antibodycrossreactivityaccountsforwidespreadappearanceofm1ain5utrs AT stevensgross antibodycrossreactivityaccountsforwidespreadappearanceofm1ain5utrs AT samierjaffrey antibodycrossreactivityaccountsforwidespreadappearanceofm1ain5utrs
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Antibody cross-reactivity accounts for widespread appearance of m1A in 5’UTRs

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