Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation

Abstract Gold standard quantitative nucleic acid tests for diagnosis of viral diseases are currently limited to implementation in laboratories outside of the clinic. An instrument for conducting nucleic acid testing at the point-of-care (POC) that is easily operable by the clinician would reduce the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dong Jin Shin, Alexander Y. Trick, Yu-Hsiang Hsieh, David L. Thomas, Tza-Huei Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b6731c948e74900bd28c83d4ac17a05
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9b6731c948e74900bd28c83d4ac17a05
record_format	dspace
spelling	oai:doaj.org-article:9b6731c948e74900bd28c83d4ac17a052021-12-02T11:40:24ZSample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation10.1038/s41598-018-28124-32045-2322https://doaj.org/article/9b6731c948e74900bd28c83d4ac17a052018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-28124-3https://doaj.org/toc/2045-2322Abstract Gold standard quantitative nucleic acid tests for diagnosis of viral diseases are currently limited to implementation in laboratories outside of the clinic. An instrument for conducting nucleic acid testing at the point-of-care (POC) that is easily operable by the clinician would reduce the required number of visits to the clinic and improve patient retention for proper treatment. Here we present a droplet magnetofluidic (DM) platform, which leverages functionalized magnetic particles to miniaturize and automate laboratory assays for use in the clinic at the POC. Our novel thermoformed disposable cartridge coupled to a portable multiaxial magnetofluidic instrument enables real-time PCR assays for quantitative and sensitive detection of nucleic acids from crude biosamples. Instead of laborious benchtop sample purification techniques followed by elution and spiking into PCR buffer, the user simply injects the biosample of interest into a cartridge with magnetic particles and loads the cartridge into the instrument. We demonstrate the utility of our platform with hepatitis C virus (HCV) RNA viral load quantitation from blood serum in approximately 1 hour. Clinical serum samples (n = 18) were directly processed on cartridges with no false positives and a limit of detection of 45 IU per 10 µl sample injection.Dong Jin ShinAlexander Y. TrickYu-Hsiang HsiehDavid L. ThomasTza-Huei WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dong Jin Shin Alexander Y. Trick Yu-Hsiang Hsieh David L. Thomas Tza-Huei Wang Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation
description	Abstract Gold standard quantitative nucleic acid tests for diagnosis of viral diseases are currently limited to implementation in laboratories outside of the clinic. An instrument for conducting nucleic acid testing at the point-of-care (POC) that is easily operable by the clinician would reduce the required number of visits to the clinic and improve patient retention for proper treatment. Here we present a droplet magnetofluidic (DM) platform, which leverages functionalized magnetic particles to miniaturize and automate laboratory assays for use in the clinic at the POC. Our novel thermoformed disposable cartridge coupled to a portable multiaxial magnetofluidic instrument enables real-time PCR assays for quantitative and sensitive detection of nucleic acids from crude biosamples. Instead of laborious benchtop sample purification techniques followed by elution and spiking into PCR buffer, the user simply injects the biosample of interest into a cartridge with magnetic particles and loads the cartridge into the instrument. We demonstrate the utility of our platform with hepatitis C virus (HCV) RNA viral load quantitation from blood serum in approximately 1 hour. Clinical serum samples (n = 18) were directly processed on cartridges with no false positives and a limit of detection of 45 IU per 10 µl sample injection.
format	article
author	Dong Jin Shin Alexander Y. Trick Yu-Hsiang Hsieh David L. Thomas Tza-Huei Wang
author_facet	Dong Jin Shin Alexander Y. Trick Yu-Hsiang Hsieh David L. Thomas Tza-Huei Wang
author_sort	Dong Jin Shin
title	Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation
title_short	Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation
title_full	Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation
title_fullStr	Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation
title_full_unstemmed	Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation
title_sort	sample-to-answer droplet magnetofluidic platform for point-of-care hepatitis c viral load quantitation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9b6731c948e74900bd28c83d4ac17a05
work_keys_str_mv	AT dongjinshin sampletoanswerdropletmagnetofluidicplatformforpointofcarehepatitiscviralloadquantitation AT alexanderytrick sampletoanswerdropletmagnetofluidicplatformforpointofcarehepatitiscviralloadquantitation AT yuhsianghsieh sampletoanswerdropletmagnetofluidicplatformforpointofcarehepatitiscviralloadquantitation AT davidlthomas sampletoanswerdropletmagnetofluidicplatformforpointofcarehepatitiscviralloadquantitation AT tzahueiwang sampletoanswerdropletmagnetofluidicplatformforpointofcarehepatitiscviralloadquantitation
_version_	1718395661538295808

Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation

Ejemplares similares