Challenges Encountered When Evaluating an Antibody-Detecting Point-of-Care Test for Taeniosis in an Endemic Community in Zambia: A Prospective Diagnostic Accuracy Study

<i>Taenia solium</i> taeniosis diagnosis is challenging because current tests perform sub-optimally and/or are expensive, require sophisticated equipment, infrastructure and trained manpower, and therefore are not community deployable. A recently-developed, multi-strip, <i>T. soliu...

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Autores principales:	Chishimba Mubanga, Chiara Trevisan, Inge Van Damme, Veronika Schmidt, Isaac K. Phiri, Gideon Zulu, John Noh, Sukwan Handali, Richard Mambo, Mwelwa Chembensofu, Maxwell Masuku, Dries Reynders, Famke Jansen, Emmanuel Bottieau, Pascal Magnussen, Andrea S. Winkler, Pierre Dorny, Kabemba E. Mwape, Sarah Gabriel
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Taenia solium</i> point of care test diagnosis taeniosis copro Ag ELISA copro PCR Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9b6a69a47204407e9420f7f27de4aa74
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spelling	oai:doaj.org-article:9b6a69a47204407e9420f7f27de4aa742021-11-25T17:21:03ZChallenges Encountered When Evaluating an Antibody-Detecting Point-of-Care Test for Taeniosis in an Endemic Community in Zambia: A Prospective Diagnostic Accuracy Study10.3390/diagnostics111120392075-4418https://doaj.org/article/9b6a69a47204407e9420f7f27de4aa742021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/2039https://doaj.org/toc/2075-4418<i>Taenia solium</i> taeniosis diagnosis is challenging because current tests perform sub-optimally and/or are expensive, require sophisticated equipment, infrastructure and trained manpower, and therefore are not community deployable. A recently-developed, multi-strip, <i>T. solium</i> point-of-care test (TS POC) for simultaneous detection of tapeworm (TS POC T) and cysticercus (TS POC CC) human antibodies was evaluated for diagnostic accuracy on consecutively recruited community participants in Sinda district, Zambia. All participants were tested using the TS POC test. All test-positives and 20% of the test-negative participants were invited to give a blood and stool sample for reference testing. Three different reference tests were used for taeniosis diagnosis: recombinant rES33 enzyme-linked immunoelectrotransfer blot (rES33 EITB), copro PCR and copro Ag ELISA. Bayesian analysis with probabilistic constraints was used to estimate sensitivity and specificity. In total, 1254 participants were tested with the TS POC test, of whom 13 tested positive using the TS POC T. Based on 161 participants with complete data, the estimated sensitivity and specificity for the TS POC T test were 38% (95% CI: 5–93%) and 99% (95% CI: 98–100%), respectively. The challenge of highly variable inter-assay performance is highlighted. We recommend either increasing the sensitivity or redesigning the test.Chishimba MubangaChiara TrevisanInge Van DammeVeronika SchmidtIsaac K. PhiriGideon ZuluJohn NohSukwan HandaliRichard MamboMwelwa ChembensofuMaxwell MasukuDries ReyndersFamke JansenEmmanuel BottieauPascal MagnussenAndrea S. WinklerPierre DornyKabemba E. MwapeSarah GabrielMDPI AGarticle<i>Taenia solium</i>point of care testdiagnosistaeniosiscopro Ag ELISAcopro PCRMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 2039, p 2039 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Taenia solium</i> point of care test diagnosis taeniosis copro Ag ELISA copro PCR Medicine (General) R5-920
spellingShingle	<i>Taenia solium</i> point of care test diagnosis taeniosis copro Ag ELISA copro PCR Medicine (General) R5-920 Chishimba Mubanga Chiara Trevisan Inge Van Damme Veronika Schmidt Isaac K. Phiri Gideon Zulu John Noh Sukwan Handali Richard Mambo Mwelwa Chembensofu Maxwell Masuku Dries Reynders Famke Jansen Emmanuel Bottieau Pascal Magnussen Andrea S. Winkler Pierre Dorny Kabemba E. Mwape Sarah Gabriel Challenges Encountered When Evaluating an Antibody-Detecting Point-of-Care Test for Taeniosis in an Endemic Community in Zambia: A Prospective Diagnostic Accuracy Study
description	<i>Taenia solium</i> taeniosis diagnosis is challenging because current tests perform sub-optimally and/or are expensive, require sophisticated equipment, infrastructure and trained manpower, and therefore are not community deployable. A recently-developed, multi-strip, <i>T. solium</i> point-of-care test (TS POC) for simultaneous detection of tapeworm (TS POC T) and cysticercus (TS POC CC) human antibodies was evaluated for diagnostic accuracy on consecutively recruited community participants in Sinda district, Zambia. All participants were tested using the TS POC test. All test-positives and 20% of the test-negative participants were invited to give a blood and stool sample for reference testing. Three different reference tests were used for taeniosis diagnosis: recombinant rES33 enzyme-linked immunoelectrotransfer blot (rES33 EITB), copro PCR and copro Ag ELISA. Bayesian analysis with probabilistic constraints was used to estimate sensitivity and specificity. In total, 1254 participants were tested with the TS POC test, of whom 13 tested positive using the TS POC T. Based on 161 participants with complete data, the estimated sensitivity and specificity for the TS POC T test were 38% (95% CI: 5–93%) and 99% (95% CI: 98–100%), respectively. The challenge of highly variable inter-assay performance is highlighted. We recommend either increasing the sensitivity or redesigning the test.
format	article
author	Chishimba Mubanga Chiara Trevisan Inge Van Damme Veronika Schmidt Isaac K. Phiri Gideon Zulu John Noh Sukwan Handali Richard Mambo Mwelwa Chembensofu Maxwell Masuku Dries Reynders Famke Jansen Emmanuel Bottieau Pascal Magnussen Andrea S. Winkler Pierre Dorny Kabemba E. Mwape Sarah Gabriel
author_facet	Chishimba Mubanga Chiara Trevisan Inge Van Damme Veronika Schmidt Isaac K. Phiri Gideon Zulu John Noh Sukwan Handali Richard Mambo Mwelwa Chembensofu Maxwell Masuku Dries Reynders Famke Jansen Emmanuel Bottieau Pascal Magnussen Andrea S. Winkler Pierre Dorny Kabemba E. Mwape Sarah Gabriel
author_sort	Chishimba Mubanga
title	Challenges Encountered When Evaluating an Antibody-Detecting Point-of-Care Test for Taeniosis in an Endemic Community in Zambia: A Prospective Diagnostic Accuracy Study
title_short	Challenges Encountered When Evaluating an Antibody-Detecting Point-of-Care Test for Taeniosis in an Endemic Community in Zambia: A Prospective Diagnostic Accuracy Study
title_full	Challenges Encountered When Evaluating an Antibody-Detecting Point-of-Care Test for Taeniosis in an Endemic Community in Zambia: A Prospective Diagnostic Accuracy Study
title_fullStr	Challenges Encountered When Evaluating an Antibody-Detecting Point-of-Care Test for Taeniosis in an Endemic Community in Zambia: A Prospective Diagnostic Accuracy Study
title_full_unstemmed	Challenges Encountered When Evaluating an Antibody-Detecting Point-of-Care Test for Taeniosis in an Endemic Community in Zambia: A Prospective Diagnostic Accuracy Study
title_sort	challenges encountered when evaluating an antibody-detecting point-of-care test for taeniosis in an endemic community in zambia: a prospective diagnostic accuracy study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9b6a69a47204407e9420f7f27de4aa74
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Challenges Encountered When Evaluating an Antibody-Detecting Point-of-Care Test for Taeniosis in an Endemic Community in Zambia: A Prospective Diagnostic Accuracy Study

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