Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis

We previously showed that mesothelial cells in human peritoneum express the water channel aquaporin 1 (AQP1) at the plasma membrane, suggesting that, although in a non-physiological context, it may facilitate osmotic water exchange during peritoneal dialysis (PD). According to the three-pore model t...

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Autores principales:	Francesca Piccapane, Andrea Gerbino, Monica Carmosino, Serena Milano, Arduino Arduini, Lucantonio Debellis, Maria Svelto, Rosa Caroppo, Giuseppe Procino
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	aquaporin 1 mesothelial cells peritoneal dialysis water transport three-pore model Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/9b76bfeb561e40f0a155314cb200e3e4
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spelling	oai:doaj.org-article:9b76bfeb561e40f0a155314cb200e3e42021-11-25T17:57:34ZAquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis10.3390/ijms2222125351422-00671661-6596https://doaj.org/article/9b76bfeb561e40f0a155314cb200e3e42021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12535https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067We previously showed that mesothelial cells in human peritoneum express the water channel aquaporin 1 (AQP1) at the plasma membrane, suggesting that, although in a non-physiological context, it may facilitate osmotic water exchange during peritoneal dialysis (PD). According to the three-pore model that predicts the transport of water during PD, the endothelium of peritoneal capillaries is the major limiting barrier to water transport across peritoneum, assuming the functional role of the mesothelium, as a semipermeable barrier, to be negligible. We hypothesized that an intact mesothelial layer is poorly permeable to water unless AQP1 is expressed at the plasma membrane. To demonstrate that, we characterized an immortalized cell line of human mesothelium (HMC) and measured the osmotically-driven transmesothelial water flux in the absence or in the presence of AQP1. The presence of tight junctions between HMC was investigated by immunofluorescence. Bioelectrical parameters of HMC monolayers were studied by Ussing Chambers and transepithelial water transport was investigated by an electrophysiological approach based on measurements of TEA<sup>+</sup> dilution in the apical bathing solution, through TEA<sup>+</sup>-sensitive microelectrodes. HMCs express Zo-1 and occludin at the tight junctions and a transepithelial vectorial Na<sup>+</sup> transport. Real-time transmesothelial water flux, in response to an increase of osmolarity in the apical solution, indicated that, in the presence of AQP1, the rate of TEA<sup>+</sup> dilution was up to four-fold higher than in its absence. Of note, we confirmed our data in isolated mouse mesentery patches, where we measured an AQP1-dependent transmesothelial osmotic water transport. These results suggest that the mesothelium may represent an additional selective barrier regulating water transport in PD through functional expression of the water channel AQP1.Francesca PiccapaneAndrea GerbinoMonica CarmosinoSerena MilanoArduino ArduiniLucantonio DebellisMaria SveltoRosa CaroppoGiuseppe ProcinoMDPI AGarticleaquaporin 1mesothelial cellsperitoneal dialysiswater transportthree-pore modelBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12535, p 12535 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aquaporin 1 mesothelial cells peritoneal dialysis water transport three-pore model Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	aquaporin 1 mesothelial cells peritoneal dialysis water transport three-pore model Biology (General) QH301-705.5 Chemistry QD1-999 Francesca Piccapane Andrea Gerbino Monica Carmosino Serena Milano Arduino Arduini Lucantonio Debellis Maria Svelto Rosa Caroppo Giuseppe Procino Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis
description	We previously showed that mesothelial cells in human peritoneum express the water channel aquaporin 1 (AQP1) at the plasma membrane, suggesting that, although in a non-physiological context, it may facilitate osmotic water exchange during peritoneal dialysis (PD). According to the three-pore model that predicts the transport of water during PD, the endothelium of peritoneal capillaries is the major limiting barrier to water transport across peritoneum, assuming the functional role of the mesothelium, as a semipermeable barrier, to be negligible. We hypothesized that an intact mesothelial layer is poorly permeable to water unless AQP1 is expressed at the plasma membrane. To demonstrate that, we characterized an immortalized cell line of human mesothelium (HMC) and measured the osmotically-driven transmesothelial water flux in the absence or in the presence of AQP1. The presence of tight junctions between HMC was investigated by immunofluorescence. Bioelectrical parameters of HMC monolayers were studied by Ussing Chambers and transepithelial water transport was investigated by an electrophysiological approach based on measurements of TEA<sup>+</sup> dilution in the apical bathing solution, through TEA<sup>+</sup>-sensitive microelectrodes. HMCs express Zo-1 and occludin at the tight junctions and a transepithelial vectorial Na<sup>+</sup> transport. Real-time transmesothelial water flux, in response to an increase of osmolarity in the apical solution, indicated that, in the presence of AQP1, the rate of TEA<sup>+</sup> dilution was up to four-fold higher than in its absence. Of note, we confirmed our data in isolated mouse mesentery patches, where we measured an AQP1-dependent transmesothelial osmotic water transport. These results suggest that the mesothelium may represent an additional selective barrier regulating water transport in PD through functional expression of the water channel AQP1.
format	article
author	Francesca Piccapane Andrea Gerbino Monica Carmosino Serena Milano Arduino Arduini Lucantonio Debellis Maria Svelto Rosa Caroppo Giuseppe Procino
author_facet	Francesca Piccapane Andrea Gerbino Monica Carmosino Serena Milano Arduino Arduini Lucantonio Debellis Maria Svelto Rosa Caroppo Giuseppe Procino
author_sort	Francesca Piccapane
title	Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis
title_short	Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis
title_full	Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis
title_fullStr	Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis
title_full_unstemmed	Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis
title_sort	aquaporin-1 facilitates transmesothelial water permeability: in vitro and ex vivo evidence and possible implications in peritoneal dialysis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9b76bfeb561e40f0a155314cb200e3e4
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Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis

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