Effects of Cadmium Sulfate on the Brown Garden Snail <i>Cornu aspersum</i>: Implications for DNA Methylation

An extensive literature exists regarding the cellular, physiological, and genetic effects of cadmium (Cd)—A highly toxic, but commonly used trace metal in modern industry. However, limited data are available on its epigenetic effects, especially for terrestrial sentinel invertebrates. We determined...

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Autores principales:	Marius Georgescu, George Andrei Drăghici, Eliza-Florentina Oancea, Cristina Adriana Dehelean, Codruţa Şoica, Nicolae-Valentin Vlăduţ, Dragoș Vasile Nica
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cadmium exposure DNA methylation 5-methylcytosine metallothioneins cadmium sulfate CdMT gene Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/9b79c0dbe2b0430a9039355a2ad9314a
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spelling	oai:doaj.org-article:9b79c0dbe2b0430a9039355a2ad9314a2021-11-25T19:08:17ZEffects of Cadmium Sulfate on the Brown Garden Snail <i>Cornu aspersum</i>: Implications for DNA Methylation10.3390/toxics91103062305-6304https://doaj.org/article/9b79c0dbe2b0430a9039355a2ad9314a2021-11-01T00:00:00Zhttps://www.mdpi.com/2305-6304/9/11/306https://doaj.org/toc/2305-6304An extensive literature exists regarding the cellular, physiological, and genetic effects of cadmium (Cd)—A highly toxic, but commonly used trace metal in modern industry. However, limited data are available on its epigenetic effects, especially for terrestrial sentinel invertebrates. We determined Cd retention, total DNA methylation, and the methylation status of 5′ end of the <i>Cd-MT</i> gene in the hepatopancreas of the brown garden snail, <i>Cornu aspersum,</i> fed Cd sulfate for four weeks. Bodyweight changes and survival were also measured. Hepatopancreas cadmium increased in a dose-dependent manner from the third-lowest dose onward, with very large amounts being found for the highest treatment group. However, no mortalities occurred, irrespective of dietary Cd dose. We identified significant genome-wide hypermethylation in specimens given the highest dose, which overlapped with a significant bodyweight decrease. The <i>Cd-MT</i> gene showed an unmethylated 5′ end of the <i>Cd-MT</i> gene and this status was not affected by cadmium exposure. Hepatopancreas DNA methylation is as sensitive as bodyweight to non-lethal concentrations of dietary Cd given as cadmium sulfate but less responsive than tissue accumulation. Such an exposure event, by contrast, does not affect the methylation status of the <i>Cd-MT</i> gene 5′ end.Marius GeorgescuGeorge Andrei DrăghiciEliza-Florentina OanceaCristina Adriana DeheleanCodruţa ŞoicaNicolae-Valentin VlăduţDragoș Vasile NicaMDPI AGarticlecadmium exposureDNA methylation5-methylcytosinemetallothioneinscadmium sulfateCdMT geneChemical technologyTP1-1185ENToxics, Vol 9, Iss 306, p 306 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cadmium exposure DNA methylation 5-methylcytosine metallothioneins cadmium sulfate CdMT gene Chemical technology TP1-1185
spellingShingle	cadmium exposure DNA methylation 5-methylcytosine metallothioneins cadmium sulfate CdMT gene Chemical technology TP1-1185 Marius Georgescu George Andrei Drăghici Eliza-Florentina Oancea Cristina Adriana Dehelean Codruţa Şoica Nicolae-Valentin Vlăduţ Dragoș Vasile Nica Effects of Cadmium Sulfate on the Brown Garden Snail <i>Cornu aspersum</i>: Implications for DNA Methylation
description	An extensive literature exists regarding the cellular, physiological, and genetic effects of cadmium (Cd)—A highly toxic, but commonly used trace metal in modern industry. However, limited data are available on its epigenetic effects, especially for terrestrial sentinel invertebrates. We determined Cd retention, total DNA methylation, and the methylation status of 5′ end of the <i>Cd-MT</i> gene in the hepatopancreas of the brown garden snail, <i>Cornu aspersum,</i> fed Cd sulfate for four weeks. Bodyweight changes and survival were also measured. Hepatopancreas cadmium increased in a dose-dependent manner from the third-lowest dose onward, with very large amounts being found for the highest treatment group. However, no mortalities occurred, irrespective of dietary Cd dose. We identified significant genome-wide hypermethylation in specimens given the highest dose, which overlapped with a significant bodyweight decrease. The <i>Cd-MT</i> gene showed an unmethylated 5′ end of the <i>Cd-MT</i> gene and this status was not affected by cadmium exposure. Hepatopancreas DNA methylation is as sensitive as bodyweight to non-lethal concentrations of dietary Cd given as cadmium sulfate but less responsive than tissue accumulation. Such an exposure event, by contrast, does not affect the methylation status of the <i>Cd-MT</i> gene 5′ end.
format	article
author	Marius Georgescu George Andrei Drăghici Eliza-Florentina Oancea Cristina Adriana Dehelean Codruţa Şoica Nicolae-Valentin Vlăduţ Dragoș Vasile Nica
author_facet	Marius Georgescu George Andrei Drăghici Eliza-Florentina Oancea Cristina Adriana Dehelean Codruţa Şoica Nicolae-Valentin Vlăduţ Dragoș Vasile Nica
author_sort	Marius Georgescu
title	Effects of Cadmium Sulfate on the Brown Garden Snail <i>Cornu aspersum</i>: Implications for DNA Methylation
title_short	Effects of Cadmium Sulfate on the Brown Garden Snail <i>Cornu aspersum</i>: Implications for DNA Methylation
title_full	Effects of Cadmium Sulfate on the Brown Garden Snail <i>Cornu aspersum</i>: Implications for DNA Methylation
title_fullStr	Effects of Cadmium Sulfate on the Brown Garden Snail <i>Cornu aspersum</i>: Implications for DNA Methylation
title_full_unstemmed	Effects of Cadmium Sulfate on the Brown Garden Snail <i>Cornu aspersum</i>: Implications for DNA Methylation
title_sort	effects of cadmium sulfate on the brown garden snail <i>cornu aspersum</i>: implications for dna methylation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9b79c0dbe2b0430a9039355a2ad9314a
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Effects of Cadmium Sulfate on the Brown Garden Snail <i>Cornu aspersum</i>: Implications for DNA Methylation

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