Simple filter microchip for rapid separation of plasma and viruses from whole blood

ShuQi Wang,1 Dusan Sarenac,1 Michael H Chen,1 Shih-Han Huang,1 Francoise F Giguel,2 Daniel R Kuritzkes,3 Utkan Demirci1,41Bio-acoustic MEMS in Medicine Laboratory, Department of Medicine, Division of Biomedical Engineering, Brigham and Women&#39;s Hospital, Harvard Medical School, Boston, MA...

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Autores principales:	Wang SQ, Sarenac D, Chen MH, Huang SH, Giguel FF, Kuritzkes DR, Demirci U
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9b7d06a8571d4d34a16fc2f71d844099
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spelling	oai:doaj.org-article:9b7d06a8571d4d34a16fc2f71d8440992021-12-02T04:23:49ZSimple filter microchip for rapid separation of plasma and viruses from whole blood1176-91141178-2013https://doaj.org/article/9b7d06a8571d4d34a16fc2f71d8440992012-09-01T00:00:00Zhttp://www.dovepress.com/simple-filter-microchip-for-rapid-separation-of-plasma-and-viruses-fro-a11019https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013ShuQi Wang,1 Dusan Sarenac,1 Michael H Chen,1 Shih-Han Huang,1 Francoise F Giguel,2 Daniel R Kuritzkes,3 Utkan Demirci1,41Bio-acoustic MEMS in Medicine Laboratory, Department of Medicine, Division of Biomedical Engineering, Brigham and Women&#39;s Hospital, Harvard Medical School, Boston, MA, USA; 2Infectious Diseases Unit, Massachusetts General Hospital, Boston, MA, USA; 3Section of Retroviral Therapeutics, Brigham and Women&#39;s Hospital, Boston, MA, USA; 4Harvard-MIT Health Sciences and Technology, Cambridge, MA, USAAbstract: Sample preparation is a significant challenge for detection and sensing technologies, since the presence of blood cells can interfere with the accuracy and reliability of virus detection at the nanoscale for point-of-care testing. To the best of our knowledge, there is not an existing on-chip virus isolation technology that does not use complex fluidic pumps. Here, we presented a lab-on-a-chip filter device to isolate plasma and viruses from unprocessed whole blood based on size exclusion without using a micropump. We demonstrated that viruses (eg, HIV) can be separated on a filter-based chip (2-&micro;m pore size) from HIV-spiked whole blood at high recovery efficiencies of 89.9% &plusmn; 5.0%, 80.5% &plusmn; 4.3%, and 78.2% &plusmn; 3.8%, for viral loads of 1000, 10,000 and 100,000 copies/mL, respectively. Meanwhile, 81.7% &plusmn; 6.7% of red blood cells and 89.5% &plusmn; 2.4% of white blood cells were retained on 2 &micro;m pore&ndash;sized filter microchips. We also tested these filter microchips with seven HIV-infected patient samples and observed recovery efficiencies ranging from 73.1% &plusmn; 8.3% to 82.5% &plusmn; 4.1%. These results are first steps towards developing disposable point-of-care diagnostics and monitoring devices for resource-constrained settings, as well as hospital and primary care settings.Keywords: microchip, filtration, virus isolation, plasma separation, point-of-careWang SQSarenac DChen MHHuang SHGiguel FFKuritzkes DRDemirci UDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5019-5028 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang SQ Sarenac D Chen MH Huang SH Giguel FF Kuritzkes DR Demirci U Simple filter microchip for rapid separation of plasma and viruses from whole blood
description	ShuQi Wang,1 Dusan Sarenac,1 Michael H Chen,1 Shih-Han Huang,1 Francoise F Giguel,2 Daniel R Kuritzkes,3 Utkan Demirci1,41Bio-acoustic MEMS in Medicine Laboratory, Department of Medicine, Division of Biomedical Engineering, Brigham and Women&#39;s Hospital, Harvard Medical School, Boston, MA, USA; 2Infectious Diseases Unit, Massachusetts General Hospital, Boston, MA, USA; 3Section of Retroviral Therapeutics, Brigham and Women&#39;s Hospital, Boston, MA, USA; 4Harvard-MIT Health Sciences and Technology, Cambridge, MA, USAAbstract: Sample preparation is a significant challenge for detection and sensing technologies, since the presence of blood cells can interfere with the accuracy and reliability of virus detection at the nanoscale for point-of-care testing. To the best of our knowledge, there is not an existing on-chip virus isolation technology that does not use complex fluidic pumps. Here, we presented a lab-on-a-chip filter device to isolate plasma and viruses from unprocessed whole blood based on size exclusion without using a micropump. We demonstrated that viruses (eg, HIV) can be separated on a filter-based chip (2-&micro;m pore size) from HIV-spiked whole blood at high recovery efficiencies of 89.9% &plusmn; 5.0%, 80.5% &plusmn; 4.3%, and 78.2% &plusmn; 3.8%, for viral loads of 1000, 10,000 and 100,000 copies/mL, respectively. Meanwhile, 81.7% &plusmn; 6.7% of red blood cells and 89.5% &plusmn; 2.4% of white blood cells were retained on 2 &micro;m pore&ndash;sized filter microchips. We also tested these filter microchips with seven HIV-infected patient samples and observed recovery efficiencies ranging from 73.1% &plusmn; 8.3% to 82.5% &plusmn; 4.1%. These results are first steps towards developing disposable point-of-care diagnostics and monitoring devices for resource-constrained settings, as well as hospital and primary care settings.Keywords: microchip, filtration, virus isolation, plasma separation, point-of-care
format	article
author	Wang SQ Sarenac D Chen MH Huang SH Giguel FF Kuritzkes DR Demirci U
author_facet	Wang SQ Sarenac D Chen MH Huang SH Giguel FF Kuritzkes DR Demirci U
author_sort	Wang SQ
title	Simple filter microchip for rapid separation of plasma and viruses from whole blood
title_short	Simple filter microchip for rapid separation of plasma and viruses from whole blood
title_full	Simple filter microchip for rapid separation of plasma and viruses from whole blood
title_fullStr	Simple filter microchip for rapid separation of plasma and viruses from whole blood
title_full_unstemmed	Simple filter microchip for rapid separation of plasma and viruses from whole blood
title_sort	simple filter microchip for rapid separation of plasma and viruses from whole blood
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/9b7d06a8571d4d34a16fc2f71d844099
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_version_	1718401263162359808

Simple filter microchip for rapid separation of plasma and viruses from whole blood

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